Protein switch engineering by domain insertion

Methods in Enzymology

Volumn 523, Issue , 2013, Pages 369-388

  Kanwar, Manu ^a   Wright, R Clay ^a   Date, Amol ^a   Tullman, Jennifer ^a   Ostermeier, Marc ^a  

^a Johns Hopkins University   (United States)

Author keywords

Allostery; Circular permutation; Directed evolution; DNase I; Inverse PCR; Protein engineering; Protein switch; S1 nuclease

Indexed keywords

DEOXYRIBONUCLEASE I; GENOMIC DNA; NUCLEASE S1;

ARTICLE; CHROMOSOME STRUCTURE; COMBINATORIAL LIBRARY; DIMERIZATION; DNA REPAIR; DNA REPLICATION ORIGIN; DNA SUPERCOILING; DNA TEMPLATE; DOUBLE STRANDED DNA BREAK; GENE FUSION; INVERSE POLYMERASE CHAIN REACTION; INVERTED REPEAT; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; ROOM TEMPERATURE;

EID: 84874088415     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-394292-0.00017-5     Document Type: Chapter

References (21)

1. W.R. Edwards, K. Busse, R.K. Allemann, and D.D. Jones Linking the functions of unrelated proteins using a novel directed evolution domain insertion method Nucleic Acids Research 36 2008 e78
2. G. Guntas, T.J. Mansell, J.R. Kim, and M. Ostermeier Directed evolution of protein switches and their application to the creation of ligand-binding proteins Proceedings of the National Academy of Sciences of the United States of America 102 2005 11224 11229 (Pubitemid 41153802)
3. G. Guntas, S.F. Mitchell, and M. Ostermeier A molecular switch created by in vitro recombination of nonhomologous genes Chemistry & Biology 11 2004 1483 1487 (Pubitemid 39527274)
4. G. Guntas, and M. Ostermeier Creation of an allosteric enzyme by domain insertion Journal of Molecular Biology 336 2004 263 273 (Pubitemid 38102347)
5. R.A. Heins, J.H. Choi, T. Sohka, and M. Ostermeier In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells PLoS One 6 11 2011 e27302
6. W. Ke, A.H. Laurent, M.D. Armstrong, Y. Chen, W.E. Smith, and J. Liang Structure of an engineered β-lactamase maltose binding protein fusion protein: Insights into heterotropic allosteric regulation PLoS One 7 2012 e39168
7. J.R. Kim, and M. Ostermeier Modulation of effector affinity by hinge region mutations also modulates switching activity in an engineered allosteric TEM1 β-lactamase Archives of Biochemistry and Biophysics 446 2006 44 51 (Pubitemid 43162390)
8. J. Liang, J.R. Kim, J.T. Boock, T.J. Mansell, and M. Ostermeier Ligand binding and allostery can emerge simultaneously Protein Science 16 2007 929 937 (Pubitemid 46683197)
9. D.M. Lilley The inverted repeat as a recognizable structural feature in supercoiled DNA molecules Proceedings of the National Academy of Sciences of the United States of America 77 1980 6468 6472
10. W.C. Lo, T. Dai, Y.Y. Liu, L.F. Wang, J.K. Hwang, and P.C. Lyu Deciphering the preference and predicting the viability of circular permutations in proteins PLoS One 7 2012 e31791
11. A.H. Lund, M. Duch, and F.S. Pedersen Increased cloning efficiency by temperature-cycle ligation Nucleic Acids Research 24 1996 800 801 (Pubitemid 26085860)
12. G.E. Meister, M. Kanwar, and M. Ostermeier Circular permutation of proteins S. Lutz, U. Bornscheuer, Protein engineering handbook 2009 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim
13. H. Ochman, A.S. Gerber, and D.L. Hartl Genetic applications of an inverse polymerase chain reaction Genetics 120 1988 621 623 (Pubitemid 18260496)
14. M. Ostermeier Engineering allosteric protein switches by domain insertion Protein Engineering, Design & Selection 18 2005 359 364 (Pubitemid 41187251)
15. N. Panayotatos, and R.D. Wells Cruciform structures in supercoiled DNA Nature 289 1981 466 470
16. J. Sambrook, and D. Russell Molecular cloning: A laboratory manual 3rd ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
17. Schrödinger, L.L.C., 2010. The PyMOL Molecular Graphics System, Version 1.3r1.
18. T. Sohka, R.A. Heins, R.M. Phelan, J.M. Greisler, C.A. Townsend, and M. Ostermeier An externally-tunable bacterial band-pass filter Proceedings of the National Academy of Sciences of the United States of America 106 2009 10135 10140
19. J. Tullman, G. Guntas, M. Dumont, and M. Ostermeier Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA Biotechnology and Bioengineering 108 2011 2535 2543
20. C.M. Wright, A. Majumdar, J.R. Tolman, and M. Ostermeier NMR characterization of an engineered domain fusion between maltose binding protein and TEM1 β-lactamase provides insight into its structure and allosteric mechanism Proteins 78 2010 1423 1430
21. C.M. Wright, R.C. Wright, J.R. Eshleman, and M. Ostermeier A protein therapeutic modality founded on molecular regulation Proceedings of the National Academy of Sciences of the United States of America 108 2011 16206 16211