Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA

Biotechnology and Bioengineering

Volumn 108, Issue 11, 2011, Pages 2535-2543

  Tullman, Jennifer ^a   Guntas, Gurkan ^a,b   Dumont, Matthew ^a   Ostermeier, Marc ^a  

^a Whiting School of Engineering   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Directed evolution; Protein engineering; Protein switch; S1 nuclease

Indexed keywords

DIRECTED EVOLUTION; GENE FUSION; GENETIC SELECTION; INVERTED REPEAT; LACTAMASES; LARGE DOMAIN; NUCLEASE ACTIVITY; NUCLEASE DIGESTION; PLASMID DNA; PROTEIN ENGINEERING; RIBOSE-BINDING PROTEINS; S1 NUCLEASE; SUPERCOILED;

BIOCHEMISTRY; BIOMOLECULES; CATALYST ACTIVITY; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENETIC ENGINEERING; LIBRARIES; PROTEINS;

DNA;

BETA LACTAMASE; NUCLEASE S1; PLASMID DNA;

ALLOSTERISM; ARTICLE; DNA LIBRARY; DOUBLE STRANDED DNA BREAK; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE FUSION; GENE INSERTION; GENE ISOLATION; GENETIC CODE; GENETIC SELECTION; MOLECULAR EVOLUTION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ENGINEERING;

ARTIFICIAL GENE FUSION; BETA-LACTAMASES; DNA; ESCHERICHIA COLI; FUNGAL PROTEINS; GENE LIBRARY; MUTAGENESIS, INSERTIONAL; PLASMIDS; PROTEINS; RECOMBINATION, GENETIC; SINGLE-STRAND SPECIFIC DNA AND RNA ENDONUCLEASES;

ESCHERICHIA COLI;

EID: 80053052797     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23224     Document Type: Article

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