Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering

Protein Science

Volumn 14, Issue 9, 2005, Pages 2304-2314

  Deuschle, Karen ^a   Okumoto, Sakiko ^a   Fehr, Marcus ^a   Looger, Loren L ^a   Kozhukh, Leonid ^a   Frommer, Wolf B ^a  

^a GEOPHYSICAL LABORATORY   (United States)

Author keywords

Biosensor; Conformational changes; FRET; Glucose; Glutamate; Molecular mechanics dynamics; Nanosensor; Neurotransmitter; New methods; Protein engineering; Structure function studies

Indexed keywords

BINDING PROTEIN; FLUORESCENT DYE; GLUCOSE; GLUTAMIC ACID; PROTEIN;

ALLOSTERISM; ARTICLE; BIOSENSOR; CHROMATOPHORE; EMPIRICISM; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIGAND BINDING; METABOLITE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARAMETER; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN FAMILY; RAT; SEPARATION TECHNIQUE; SIGNAL TRANSDUCTION;

BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; FLUORESCENCE RESONANCE ENERGY TRANSFER; GLUCOSE; GLUTAMIC ACID; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; METABOLISM; PERIPLASMIC BINDING PROTEINS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 24344451972     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051508105     Document Type: Article

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