Novel metagenome-derived carboxylesterase that hydrolyzes β-lactam antibiotics

Applied and Environmental Microbiology

Volumn 77, Issue 21, 2011, Pages 7830-7836

  Jeon, Jeong Ho ^a,b   Kim, Soo Jin ^d   Lee, Hyun Sook ^a   Cha, Sun Shin ^a   Lee, Jung Hun ^c   Yoon, Sang Hong ^d   Koo, Bon Sung ^d   Lee, Chang Muk ^d   Choi, Sang Ho ^b   Lee, Sang Hee ^c   Kang, Sung Gyun ^a   Lee, Jung Hyun ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^b Seoul National University   (South Korea)

^c Myong Ji University   (South Korea)

^d National Institute of Animal Science   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR PLATES; AMIDE BOND; CARBOXYLESTERASES; CATALYTIC TRIAD; CEPHALOTHIN; CHROMOGENIC SUBSTRATE; ESTER BONDS; ESTERASE ACTIVITIES; FUNCTIONAL SCREENING; HYDROLYTIC ACTIVITIES; KINETIC STUDY; LACTAMASES; LIPOLYTIC ACTIVITY; METAGENOMES; METAGENOMIC LIBRARIES; NITROCEFIN; OPEN READING FRAME; RECOMBINANT PROTEIN; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; SUBSTRATE AFFINITY; TRIBUTYRIN;

AMIDES; AMINO ACIDS; CLONING; ESCHERICHIA COLI; ESTERIFICATION; ESTERS; GENES; HYDROLYSIS; SUBSTRATES;

ANTIBIOTICS;

ANTIINFECTIVE AGENT; BETA LACTAM; CARBOXYLESTERASE; MUTANT PROTEIN; RECOMBINANT PROTEIN;

ANTIBIOTICS; CATALYSIS; ENZYME ACTIVITY; ESTER; GENOME; GENOMICS; HYDROLYSIS; MUTAGENICITY; PEPTIDE; PHYLOGENETICS; RECOMBINATION;

ARTICLE; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; GENETICS; HYDROLYSIS; KINETICS; METABOLISM; METAGENOME; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ANTI-BACTERIAL AGENTS; BETA-LACTAMS; CARBOXYLESTERASE; CATALYTIC DOMAIN; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; HYDROLYSIS; KINETICS; METAGENOME; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY; SUBSTRATE SPECIFICITY;

EID: 83155189236     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05363-11     Document Type: Article

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