Effect of Fc-Glycan Structure on the Conformational Stability of IgG Revealed by Hydrogen/Deuterium Exchange and Limited Proteolysis

Biochemistry

Volumn 55, Issue 6, 2016, Pages 860-868

  Fang, Jing ^a   Richardson, Jason ^a   Du, Zhimei ^b   Zhang, Zhongqi ^a,c,d  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

^c WATERS CORPORATION   (United States)

^d NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MASS SPECTROMETRY; MOLECULES; POLYSACCHARIDES; STABILITY; THERMODYNAMIC STABILITY;

CHINESE HAMSTER OVARY CELLS; CONFORMATIONAL FLEXIBILITY; CONFORMATIONAL STABILITIES; ENZYMATIC DEGRADATION; HYDROGEN/DEUTERIUM EXCHANGE; LIMITED PROTEOLYSIS; MOLECULAR MECHANISM; PROTEOLYTIC DEGRADATION;

CONFORMATIONS;

FC RECEPTOR; GLYCAN; GLYCOPEPTIDE; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; MANNOSE; TRYPSIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; POLYSACCHARIDE;

ANIMAL CELL; ARTICLE; CHEMICAL STRUCTURE; CHO CELL LINE; CONTROLLED STUDY; DEUTERIUM HYDROGEN EXCHANGE; ENZYMATIC DEGRADATION; MASS SPECTROMETRY; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMOSTABILITY; ANIMAL; CHEMISTRY; CONFORMATION; CRICETULUS; HAMSTER; HUMAN; PROCEDURES; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CRYSTALLOGRAPHY, X-RAY; DEUTERIUM EXCHANGE MEASUREMENT; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR CONFORMATION; POLYSACCHARIDES; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; PROTEOLYSIS;

EID: 84958811707     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.5b01323     Document Type: Article

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