Glycoforms of immunoglobulin g based biopharmaceuticals are differentially cleaved by trypsin due to the glycoform influence on higher-order structure

Journal of Proteome Research

Volumn 14, Issue 9, 2015, Pages 4019-4028

  Falck, David ^a   Jansen, Bas C ^a   Plomp, Rosina ^a   Reusch, Dietmar ^b   Haberger, Markus ^b   Wuhrer, Manfred ^a,c  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b ROCHE DIAGNOSTICS GMBH   (Germany)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

biopharmaceuticals; glycoproteomics; glycosylation; higher order structure; immunoglobulin G; method development; monoclonal antibodies; proteolytic biases; trypsin substrate specificity; tryptic cleavage

Indexed keywords

GLYCAN; IMMUNOGLOBULIN G; MANNOSE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; TRYPSIN; GLYCOPEPTIDE;

ALKYLATION; ANTIBODY STRUCTURE; ARTICLE; DENATURATION; ELECTROSPRAY MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN GLYCOSYLATION; TANDEM MASS SPECTROMETRY; THERMOSTABILITY; ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; GLYCOSYLATION; HAMSTER; METABOLISM; PIG; PROCEDURES; PROTEIN DENATURATION; PROTEOMICS;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; GLYCOPEPTIDES; GLYCOSYLATION; IMMUNOGLOBULIN G; PROTEIN DENATURATION; PROTEOMICS; SWINE; TRYPSIN;

EID: 84941057683     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/acs.jproteome.5b00573     Document Type: Article

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