Plasticity of quaternary structure: Twenty-two ways to form a LacI dimer

Protein Science

Volumn 10, Issue 2, 2001, Pages 262-276

  Swint Kruse, Liskin ^a   Elam, Corey R ^a   Lin, Jennifer W ^a   Wycuff, Diane R ^a,b   Matthews, Kathleen Shive ^a  

^a RICE UNIVERSITY   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Lactose repressor protein; Periplasmic binding protein; Purine repressor protein; Quaternary structure; Structural plasticity; Trehalose repressor protein

Indexed keywords

BETA GALACTOSIDASE; BINDING PROTEIN; DIMER; DNA; LACTOSE; OLIGOMER; PERIPLASMIC BINDING PROTEIN; PROTEIN LACI; REPRESSOR PROTEIN; TREHALOSE; UNCLASSIFIED DRUG;

ALLOSTERISM; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DIMERIZATION; ESCHERICHIA COLI; NONHUMAN; OLIGOMERIZATION; PHENOTYPE; PLASTICITY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN QUATERNARY STRUCTURE;

ALLOSTERIC SITE; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; DIMERIZATION; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PLASMIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; WATER;

ESCHERICHIA COLI;

EID: 0035108019     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.35801     Document Type: Article

References (54)

1. Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat
2. Genetic analysis of the leucine heptad repeats of Lac repressor: Evidence for a 4-helical bundle
3. Basic local alignment search tool
4. Characterization of cytR mutations that influence oligomerization of mutant repressor subunits
5. Substitutions at histidine 74 and aspartate 278 alter ligand binding and allostery in lactose repressor protein
6. A closer view of the conformation of the Lac repressor bound to operator
7. Multiple open forms of ribose-binding protein trace the path of its conformational change
8. Characterization of mutations in oligomerization domain of lac repressor protien
9. Evidence for leucine zipper motif in lactose repressor protein
10. Lysine 84 is at the subunit interface of lac repressor protein
11. Identification and characterization of aspartate residues that play key roles in the allosteric regulation of a transcription factor: Aspartate 274 is essential for inducer binding in lac repressor
12. Structure of D-allose binding protein from Escherichia coli bound to D-allose at 1.8 Å resolution
13. Deletion of lactose repressor carboxylterminal domain affects tetramer formation
14. 282→ Asp
15. Structure of the complex of lac repressor headpiece and an 11 base-pair half-operator determined by nuclear magnetic resonance spectroscopy and restrained molecular dynamics
16. Characterization and modification of a monomeric mutant of the lactose repressor protein
17. Formation of mixed disulfide adducts at cysteine-281 of the lactose repressor protein affects operator and inducer binding parameters
18. Dimerisation mutants of lac repressor. I. A monomeric mutant, L251A, that binds lac operator DNA as a dimer
19. Limited proteolytic digestion of lac repressor by trypsin: Chemical nature of the resulting trypsin-resistant core
20. Crystal structure of lac repressor core tetramer and its implications for DNA looping
21. Isolation of the amino-terminal fragment of lactose repressor necessary for DNA binding
22. Strengthening the dimerisation interface of lac repressor increases its thermostability by 40 deg C
23. Crystal structure of the effector-binding domain of the trehalose-repressor of Escherichia coli, a member of the LacI family, in its complexes with inducer trehalose-6-phosphate and noninducer trehalose
24. Mapping the protein universe
25. Amino-terminal fragments of Escherichia coli lac repressor bind to DNA
26. In vivo interaction of Escherichia coli lac repressor N-terminal fragments with the lac operator
27. Genetic studies of the lac repressor. XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors
28. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
29. Escherichia coli purine repressor: Key residues for the allosteric transition between active and inactive conformations and for interdomain signaling
30. Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence
31. Lactose repressor protein: Functional properties and structure
32. Relieving repression
33. Conformational changes of ribosebinding protein and two related repressors are tailored to fit the functional need
34. 1.7Å X-ray structure of the periplasmic ribose receptor from Escherichia coli
35. Sequence homology between Lac and Gal repressors and three sugar-binding periplasmic proteins
36. L-Arabinose-binding protein-sugar complex at 2.4Å resolution
37. Combinatorial mutations of lac repressor: Stability of monomer-monomer interface is increased by apolar substitution at position 84
38. Model of lactose repressor core based on alignment with sugar-binding proteins is concordant with genetic and chemical data
39. An amino-terminal fragment of lac repressor binds specifically to lac operator
40. 2-terminal region and loss of the deoxyribonucleic acid-binding activity
41. Novel stereospecificity of the L-arabinose-binding protein
42. Predicted structure of the sugar-binding site of the lac repressor
43. Mutations affecting the quaternary structure of the lac repressor
44. Crystal structure of LacI member, PurR, bound to DNA: Minor groove binding by a helices
45. Mechanism of corepressor-mediated specific DNA binding by the purine repressor
46. Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: Change of tertiary structure upon binding to the lac operator
47. Dimerisation mutants of lac repressor. II. A single amino acid substitution, D278L, changes the specificity of dimerisation
48. Genetic studies of the lac repressor XV: 4000 Single amino acid substitutions and analysis of the resulting phenotypes on the basis of the protein structure
49. Contemporary approaches to protein structure classification
50. Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli
51. Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein
52. Comparison of the periplasmic receptors for L-arabinose, D-glucose/D-galactose, and D-ribose: Structural and functional similarity
53. A family of bacterial regulators homologous to Gal and Lac repressors