Rosetta comparative modeling for library design: Engineering alternative inducer specificity in a transcription factor

Proteins: Structure, Function and Bioinformatics

Volumn 83, Issue 7, 2015, Pages 1327-1340

  Jha, Ramesh K ^a   Chakraborti, Subhendu ^a   Kern, Theresa L ^a   Fox, David T ^a   Strauss, Charlie E M ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

Altered specificity; BLOSUM62; Comparative modeling; Ligand docking; PobR; Protein engineering; Rosetta; Transcription factor

Indexed keywords

3,4 DIHYDROXY BENZOATE; 4 HYDROXYBENZOIC ACID; DIMER; GREEN FLUORESCENT PROTEIN; HYDROXYBENZOIC ACID DERIVATIVE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; 3,4-DIHYDROXYBENZOATE; 4 HYDROXYBENZOIC ACID ESTER; 4-HYDROXYBENZOIC ACID; BACTERIAL PROTEIN; HYBRID PROTEIN; LIGAND; PEPTIDE LIBRARY; POBR PROTEIN, ACINETOBACTER CALCOACETICUS; PROTEIN BINDING; SALICYLIC ACID; TRANSACTIVATOR PROTEIN;

ACINETOBACTER; ARTICLE; CRYSTAL STRUCTURE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GAIN OF FUNCTION MUTATION; MASS SPECTROMETRY; MOLECULAR DOCKING; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PEPTIDE LIBRARY; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; SINGLE CELL ANALYSIS; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; DRUG EFFECTS; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PROCEDURES; PROMOTER REGION; REPORTER GENE;

ACINETOBACTER;

ACINETOBACTER; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; ESCHERICHIA COLI; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HYDROXYBENZOATES; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PARABENS; PEPTIDE LIBRARY; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SALICYLIC ACID; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

EID: 84930999631     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.24828     Document Type: Article

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