Highly efficient production of VHH antibody fragments in Brevibacillus choshinensis expression system

Protein Expression and Purification

Volumn 105, Issue , 2015, Pages 23-32

  Mizukami, Makoto ^a   Tokunaga, Hiroko ^b   Onishi, Hiromasa ^a   Ueno, Yohei ^a   Hanagata, Hiroshi ^a   Miyazaki, Nobuo ^b   Kiyose, Norihiko ^b   Ito, Yuji ^b   Ishibashi, Matsujiro ^b   Hagihara, Yoshihisa ^c   Arakawa, Tsutomu ^d   Miyauchi, Akira ^a   Tokunaga, Masao ^b  

^a Higeta Shoyu Co Ltd   (Japan)

^b KAGOSHIMA UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

Antibody fragments; Brevibacillus choshinensis; Heavy chain antibody; Reversible denaturation; Secretory production; VHH

Indexed keywords

BREVIBACILLUS CHOSHINENSIS; CAMELIDAE; LAMA PACOS;

ANTIBODY; NANOBODY; RECOMBINANT PROTEIN;

BREVIBACILLUS; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN RENATURATION;

ANTIBODIES; BREVIBACILLUS; PROTEIN RENATURATION; RECOMBINANT PROTEINS; SINGLE-DOMAIN ANTIBODIES;

EID: 84949116897     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.09.017     Document Type: Article

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