High production of llama variable heavy-chain antibody fragment (VHH) fused to various reader proteins by Aspergillus oryzae

Applied Microbiology and Biotechnology

Volumn 97, Issue 2, 2013, Pages 761-766

  Hisada, Hiromoto ^a   Tsutsumi, Hiroko ^a   Ishida, Hiroki ^a   Hata, Yoji ^a  

^a Gekkeikan Sake Company Ltd   (Japan)

Author keywords

Aspergillus oryzae; His tagged protein; Llama variable heavy chain antibody fragment (VHH); Overproduction; Reader protein

Indexed keywords

ASPERGILLUS ORYZAE; CULTURE MEDIUM; FUSION PROTEINS; GLUCOAMYLASE; HEAVY-CHAIN ANTIBODY FRAGMENTS; HIGH MOLECULAR WEIGHT; HIS-TAG; HIS-TAGGED PROTEINS; HUMAN CHORIONIC GONADOTROPINS; N-TERMINALS; OVERPRODUCTION; POTASSIUM PHOSPHATE; PRODUCTION OF; SDS-PAGE ANALYSIS; TETRAMETHYLBENZIDINE;

AFFINITY CHROMATOGRAPHY; ASPERGILLUS; GLUCOSE SENSORS; MOLECULAR WEIGHT; PURIFICATION;

ANTIBODIES;

ANTIBODY; BOVINE SERUM ALBUMIN; CHORIONIC GONADOTROPIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE OXIDASE; LLAMA VARIABLE HEAVY CHAIN ANTIBODY FRAGMENT ANTIBODY; MALTOSE; PEROXIDASE; POTASSIUM DIHYDROGEN PHOSPHATE; UNCLASSIFIED DRUG;

ANTIBODY; CHROMATOGRAPHY; FUNGUS; MAMMAL; MOLECULAR ANALYSIS; PROTEIN; RUMINANT; SUBSTRATE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANTIBODY PRODUCTION; ARTICLE; ASPERGILLUS ORYZAE; BINDING AFFINITY; BIOTRANSFORMATION; CARBOXY TERMINAL SEQUENCE; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBSTRATE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; WILD TYPE;

ANIMALS; ASPERGILLUS ORYZAE; CAMELIDS, NEW WORLD; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; RECOMBINANT FUSION PROTEINS;

ASPERGILLUS ORYZAE; LAMA (MAMMAL);

EID: 84873992167     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4211-0     Document Type: Article

References (27)

1. Abramoff MD, Magalhaes PJ, Ram SJ (2004) Image processing with ImageJ. Biophoton Int 11:36-42
2. Archer DB, Peberdy JF (1997) The molecular biology of secreted enzyme production by fungi. Crit Rev Biotechnol 17:273-306
3. Bando H, Hisada H, Ishida H, Hata Y, Katakura Y, Kondo A (2011) Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture. Appl Microbiol Biotechnol 92:561-569
4. Carvalho JJ, Weller MG, Panne U, Schneider RJ (2010) A highly sensitive caffeine immunoassay based on a monoclonal antibody. Anal Bioanal Chem 396:2617-2628
5. Christensen ME, Engbaek F, Therkildsen MH, Bretlau P, Nexø E (1995) A sensitive enzyme-linked immunosorbent assay used for quantitation of epidermal growth factor receptor protein in head and neck carcinomas: evaluation, interpretations and limitations. Br J Cancer 72:1487-1493
6. Flajnik MF, Deschacht N, Muyldermans S (2011) A case of convergence: why did a simple alternative to canonical antibodies arise in sharks and camels? PLoS Biol 9:e1001120
7. Gomi K, Iimura Y, Hara S (1987) Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene. Agric Biol Chem 51:2549-2555
8. Hagihara Y, Matsuda T, Yumoto N (2005) Cellular quality control screening to identify amino acid pairs for substituting the disulfide bonds in immunoglobulin fold domains. J Biol Chem 280:24752-24758
9. Hagihara Y, Mine S, Uegaki K (2007) Stabilization of an immunoglobulin fold domain by an engineered disulfide bond at the buried hydrophobic region. J Biol Chem 282:36489-36495
10. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB, Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
11. Hirayama K, Watanabe H, Tokuda G, Kitamoto K, Arioka M (2010) Purification and characterization of termite endogenous β-1,4- endoglucanases produced in Aspergillus oryzae. Biosci Biotechnol Biochem 74:1680-1686
12. Hussack G, Hirama T, Ding W, MacKenzie R, Tanha J (2011) Engineered single-domain antibodies with high protease resistance and thermal stability. PLoS One 6:e28218. doi: 10.1371/journal.pone.0028218
13. Ishida H, Hata Y, Kawato A, Abe Y, Kashiwagi Y (2006) Novel auxotrophic mutants and selection marker genes of Aspergillus oryzae. Jpn Patent Kokai 2006-345817
14. HHs) by Aspergillus awamori. Appl Microbiol Biotechnol 66:384-392
15. HH) R9 coupled to Arthromyces ramosus peroxidase (ARP). J Biotechnol 120:347-359
16. Krüger C, Hultberg A, Marcotte H, Hermans P, Bezemer S, Frenken LG, Hammarstrom L (2006) Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries. Appl Microbiol Biotechnol 72:732-737
17. Meyer V, Wu B, Ram AF (2011) Aspergillus as a multi-purpose cell factory: current status and perspectives. Biotechnol Lett 33:469-476
18. Muyldermans S (2001) Single domain camel antibodies: current status. J Biotechnol 74:277-302
19. Nishimoto N, Yoshizaki K, Maeda K, Kuritani T, Deguchi H, Sato B, Imai N, Suemura M, Kakehi T, Takagi N, Kishimoto T (2003) Toxicity, pharmacokinetics, and dose-finding study of repetitive treatment with the humanized anti-interleukin 6 receptor antibody MRA in rheumatoid arthritis. Phase I/II clinical study. J Rheumatol 30:1426-1435
20. Nora K, Jason E, Mark L (2010) Review of ustekinumab, an interleukin-12 and interleukin-23 inhibitor used for the treatment of plaque psoriasis. Ther Clin Risk Manag 6:123-141
21. Ohno A, Maruyama JI, Nemoto T, Arioka M, Kitamoto K (2011) A carrier fusion significantly induces unfolded protein response in heterologous protein production by Aspergillus oryzae. Appl Microbiol Biotechnol 92:1197-1206
22. Ponmariappan S, Jain S, Sijoria R, Tomar A, Kumar O (2011) Expression, purification and development of neutralizing antibodies from synthetic BoNT/B LC and its application in detection of botulinum toxin serotype B. Protein Pept Lett. PMID 21933129
23. Roth AH, Dersch P (2010) A novel expression system for intracellular production and purification of recombinant affinity-tagged proteins in Aspergillus niger. Appl Microbiol Biotechnol 86:659-670
24. Sato H, Toyoshima Y, Shintani T, Gomi K (2011) Identification of potential cell wall component that allows Taka-amylase A adsorption in submerged cultures of Aspergillus oryzae. Appl Microbiol Biotechnol 92:961-969
25. Spector S (2004) Omalizumab: efficacy in allergic disease. Panminerva Med 46:141-148
26. Uchigashima M, Saigusa M, Yamashita H, Miyake S, Fujita K, Nakajima M, Nishijima M (2009) Development of a novel immunoaffinity column for aflatoxin analysis using an organic solvent-tolerant monoclonal antibody. J Agric Food Chem 57:8728-8734
27. Yoon J, Maruyama J, Kitamoto K (2011) Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins. Appl Microbiol Biotechnol 89:747-759