Efficient production of anti-fluorescein and anti-lysozyme as single-chain anti-body fragments (scFv) by Brevibacillus expression system

Protein Expression and Purification

Volumn 91, Issue 2, 2013, Pages 184-191

  Onishi, Hiromasa ^a   Mizukami, Makoto ^a   Hanagata, Hiroshi ^a   Tokunaga, Masao ^b   Arakawa, Tsutomu ^c   Miyauchi, Akira ^a  

^a Higeta Shoyu Co Ltd   (Japan)

^b KAGOSHIMA UNIVERSITY   (Japan)

^c ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

Brevibacillus choshinensis; Expression system; Recombinant protein; Secretory expression; Signal peptide; Single chain antibody (scFv)

Indexed keywords

FLUORESCEIN; LYSOZYME; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ARTICLE; BIOSYNTHESIS; BREVIBACILLUS; BREVIBACILLUS CHOSHINENSIS; CHEMISTRY; GEL CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; GENETICS; METABOLISM; SECRETORY EXPRESSION; SINGLE-CHAIN ANTIBODY (SCFV);

BREVIBACILLUS CHOSHINENSIS; EXPRESSION SYSTEM; RECOMBINANT PROTEIN; SECRETORY EXPRESSION; SIGNAL PEPTIDE; SINGLE-CHAIN ANTIBODY (SCFV);

BREVIBACILLUS; CHROMATOGRAPHY, GEL; FLUORESCEIN; MURAMIDASE; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84883514101     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.08.005     Document Type: Article

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