Efficient expression, purification and characterization of mouse salivary α-amylase secreted from methylotrophic yeast, Pichia pastoris

Yeast

Volumn 18, Issue 7, 2001, Pages 643-655

  Kato, Shinya ^a   Ishibashi, Matsujiro ^a   Tatsuda, Daisuke ^a   Tokunaga, Hiroko ^a   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

Glycosylation; Mouse amylase; pGKL killer protein; Pichia pastoris; Production; Secretion; Signal sequence

Indexed keywords

AMYLASE; PROTEIN; SIGNAL PEPTIDE;

ARTICLE; CONTROLLED STUDY; DNA VECTOR; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; FUNGUS GROWTH; GENE CASSETTE; GENE INSERTION; GENETIC TRANSFORMATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; MOLECULE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; SPECIES; TEMPERATURE; YEAST;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; GLYCOSIDE HYDROLASES; GLYCOSYLATION; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, PROTEIN;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 0034981464     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.714     Document Type: Article

References (21)

1. Reconstitution of SEC gene product-dependent intercompartmental protein transport
2. Heterologous protein expression in the methylotrophic yeast Pichia pastoris
3. Asparagine as a nitrogen source for improving the secretion of mouse alpha-amylase in Saccharomyces cerevisiae protease A-deficient strains
4. Genetic variation in mouse salivary amylase rate of synthesis
5. Production of recombinant proteins by methylotrophic yeasts
6. Yeast alpha factor is processed from a larger precursor polypeptide: The essential role of a membrane-bound dipeptidyl aminopeptidase
7. Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae
8. Isolation and characterization of kar2-404 mutation in Saccharomyces cerevisiae
9. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
10. Protein measurement with the folin phenol reagent
11. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris
12. Biochemical and immunochemical studies of alpha-amylase from the salivary glands of the mouse
13. Foreign gene expression in yeast: A review
14. A novel yeast secretion vector utilizing secretion signal of killer toxin encoded on the yeast linear DNA plasmid pGKL1
15. A novel secretion signal isolated from 28 K killer precursor protein encoded on the linear DNA plasmid pGKL1
16. Characterization of mouse α-amylase secreted from Saccharomyces cerevisiae by the pGKL 128 kDa killer secretion signal
17. Structure of yeast pGKL 128 kDa killer toxin secretion signal sequence. Processing of the 128 kDa killer toxin secretion signal-alpha-amylase fusion protein
18. Secretion of mouse α-amylase from fission yeast Schizosaccharomyces pombe: Presence of chymostatine-sensitive protease activity in the culture medium
19. Secretion of mouse α-amylase from Kluyveromyces lactis
20. Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris