A polysaccharide from pumpkin induces apoptosis of HepG2 cells by activation of mitochondrial pathway

Tumor Biology

Volumn 37, Issue 4, 2016, Pages 5239-5245

  Shen, Weixi ^a   Guan, Yuanyuan ^b   Wang, Jingfang ^a   Hu, Yu ^a   Tan, Qian ^a   Song, Xiaowei ^a   Jin, Yinghua ^a   Liu, Ying ^a   Zhang, Yanqiao ^c  

^a THE SECOND AFFILIATED HOSPITAL OF HARBIN MEDICAL UNIVERSITY   (China)

^b THE FIRST AFFILIATED HOSPITAL OF HARBIN MEDICAL UNIVERSITY   (China)

^c HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Hepatoma; Mitochondria; Polysaccharide; Pumpkin; Purification

Indexed keywords

CASPASE 3; CASPASE 9; CYTOCHROME C; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; POLYSACCHARIDE; PROTEIN BAX; PROTEIN BCL 2;

ANTIPROLIFERATIVE ACTIVITY; APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL DEATH; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOSOL; DRUG ISOLATION; DRUG PURIFICATION; ENZYME ACTIVITY; HEPG2 CELL LINE; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; SQUASH; WESTERN BLOTTING; CARCINOMA, HEPATOCELLULAR; CHEMISTRY; DRUG EFFECTS; GENETICS; HEP-G2 CELL LINE; LIVER NEOPLASMS; MITOCHONDRION; PATHOLOGY; SIGNAL TRANSDUCTION;

APOPTOSIS; CARCINOMA, HEPATOCELLULAR; CASPASE 3; CUCURBITA; HEP G2 CELLS; HUMANS; LIVER NEOPLASMS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; POLYSACCHARIDES; SIGNAL TRANSDUCTION;

EID: 84946761250     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1007/s13277-015-4338-6     Document Type: Article

References (34)

1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61:69–90.
2. Chen CJ, Yu MW, Liaw YF. Epidemiological characteristics and risk factors of hepatocellular carcinoma. J Gastroenterol Hepatol. 1997;12:S294–308.
3. Zhao X, Shu G, Chen L, Mi X, Mei Z, Deng X. A flavonoid component from Docynia delavayi (Franch.) Schneid represses transplanted H22 hepatoma growth and exhibits low toxic effect on tumor-bearing mice. Food Chem Toxicol. 2012;50:3166–73.
4. Yu J, Liu H, Lei J, Tan W, Hu X, Zou G. Antitumor activity of chloroform fraction of Scutellaria barbata and its active constituents. Phytother Res. 2007;21:817–22.
5. Zou Y, Xiong H, Xiong H, Lu T, Zhu F, Luo Z, et al. A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice. Tumour Biol. 2015;36:2929–36.
6. Wang SY, Huang WC, Liu CC, Wang MF, Ho CS, Huang WP, et al. Pumpkin (Cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice. Molecules. 2012;17:11864–76.
7. Nara K, Yamaguchi A, Maeda N, Koga H. Antioxidative activity of water soluble polysaccharide in pumpkin fruits (Cucurbita maxima Duchesne). Biosci Biotechnol Biochem. 2009;73:1416–8.
8. Xiong X, Cao J. Study of extraction and isolation of effective pumpkin polysaccharide component and its reducing glycemia function. Chin J Mod Appl Pharm. 2001;18:662–4.
9. Zhang Y, Yao H. Study on effect of hypoglycemia of different type pumpkin. J Chin Food Sci. 2002;23:118–20.
10. Quanhong L, Caili F, Yukui R, Guanghui H, Tongyi C. Effects of protein-bound polysaccharide isolated from pumpkin on insulin in diabetic rats. Plant Foods Hum Nutr. 2005;60:13–6.
11. Fu CL, Shi H, Li QH. A review on pharmacological activities and utilization technologies of pumpkin. Plant Foods Hum Nutr. 2006;61:73–80.
12. Staub AM. Removal of protein—Sevag method. Methods Carbohydr Chem. 1965;5:5–6.
13. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350–6.
14. Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973;54:484–9.
15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Anal Biochem. 1976;72:248–54.
16. Erbing B, Jansson PE, Widmalm G, Nimmich W. Structure of the capsular polysaccharide from the Klebsiella K8 reference strain 1015. Carbohydr Res. 1995;273:197–205.
17. Guentas L, Pheulpin P, Michaud P, Heyraud A, Gey C, Courtois B, et al. Structure of a polysaccharide from a Rhizobium species containing 2-deoxy-beta-D-arabino-hexuronic acid. Carbohydr Res. 2001;332:167–73.
18. Zhang F, Song X, Li L, Wang J, Lin L, Li C, et al. Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity. Tumour Biol. 2015;36:2907–12.
19. Zhang F, Song X, Li L, Wang J, Lin L, Li C, et al. Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway. Tumour Biol. 2015;36:2913–9.
20. Miao S, Mao X, Pei R, Miao S, Xiang C, Lv Y, et al. Lepista sordida polysaccharide induces apoptosis of Hep-2 cancer cells via mitochondrial pathway. Int J Biol Macromol. 2013;61:97–101.
21. Zhang Y, Sun S, Chen J, Ren P, Hu Y, Cao Z, et al. Oxymatrine induces mitochondria dependent apoptosis in human osteosarcoma MNNG/HOS cells through inhibition of PI3K/Akt pathway. Tumour Biol. 2014;35:1619–25.
22. Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, et al. Molecular characterization of mitochondrial apoptosis-inducing factors. Nature. 1999;397:441–6.
23. Green DR. Apoptotic pathways: the roads to ruin. Cell. 1998;94:695–8.
24. Hengartner MO. The biochemistry of apoptosis. Nature. 2006;407:770–6.
25. Orrenius S. Mitochondrial regulation of apoptotic cell death. Toxicol Lett. 2004;149:19–23.
26. Scorrano L, Korsmeyer SJ. Mechanisms of cytochrome c release by proapoptotic Bcl-2 family members. Biochem Biophys Res Commun. 2003;304:437–44.
27. Kluck R, Bossy-Wetzel E, Green DR, Newmeyer DD. The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science. 1997;275:1132–6.
28. Shen DY, Kang JH, Song W, Zhang WQ, Li WG, Zhao T, et al. Apoptosis of human cholangiocarcinoma cell lines induced by β-escin through mitochondrial caspase-dependent pathway. Phytother Res. 2011;25:1519–26.
29. Sun Z, Huang K, Fu X, Zhou Z, Cui Y, Li H. A chemically sulfated polysaccharide derived from Ganoderma lucidum induces mitochondrial-mediated apoptosis in human osteosarcoma MG63 cells. Tumour Biol. 2014;35:9919–26.
30. Yakovlev AG, Wang G, Stoica BA, Boulares HA, Spoonde AY, Yoshihara K, et al. A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by poly (ADP-ribose) polymerase. J Biol Chem. 2000;275:21302–8.
31. Qin H, Du XY, Zhang Y, Wang R, Platycodin D. A triterpenoid saponin from Platycodon grandiflorum, induces G2/M arrest and apoptosis in human hepatoma HepG2 cells by modulating the PI3K/Akt pathway. Tumour Biol. 2014;35:1267–74.
32. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997;91:479–89.
33. Wang X, Chen Y, Wang J, Liu Z, Zhao S. Antitumor activity of a sulfated polysaccharide from Enteromorpha intestinalis targeted against hepatoma through mitochondrial pathway. Tumour Biol. 2014;35:1641–7.
34. Decker P, Muller S. Modulating poly (ADP-ribose) polymerase activity: potential for the prevention and therapy of pathogenic situations involving DNA damage and oxidative stress. Curr Pharm Biotechnol. 2002;3:275–83.