Platycodin D, a triterpenoid saponin from Platycodon grandiflorum, inducesG2/M arrest and apoptosis in human hepatoma HepG2 cells by modulating the PI3K/Akt pathway

Tumor Biology

Volumn 35, Issue 2, 2014, Pages 1267-1274

  Qin, Hua ^a   Du, Xiaoyan ^a   Zhang, Yan ^a   Wang, Ru ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antitumor; Hepatoma; HepG2 cells; PI3K Akt signaling pathway; Platycodin D; Platycodon grandiflorum

Indexed keywords

CASPASE 3; CASPASE 9; PHOSPHATIDYLINOSITOL 3 KINASE; PLATYCODIN; PLATYCODON GRANDIFLORUM EXTRACT; PROTEIN BCL 2; PROTEIN KINASE B; SAPONIN; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; ANTITUMOR GENE; APOPTOSIS; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; DOWN REGULATION; FLOW CYTOMETRY; G2 PHASE CELL CYCLE CHECKPOINT; HUMAN; HUMAN CELL; MTT ASSAY; NONHUMAN; PLATYCODON; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; UPREGULATION; WESTERN BLOTTING;

APOPTOSIS; CARCINOMA, HEPATOCELLULAR; CELL CYCLE CHECKPOINTS; ELAFIN; G2 PHASE CELL CYCLE CHECKPOINTS; HEP G2 CELLS; HUMANS; LIVER NEOPLASMS; PLATYCODON; PROTO-ONCOGENE PROTEINS C-AKT; SAPONINS; SIGNAL TRANSDUCTION; TRITERPENES;

EID: 84899045505     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1007/s13277-013-1169-1     Document Type: Article

References (35)

1. Masuzaki R, Omata M. Treatment of hepatocellular carcinoma. Indian J Gastroenterol. 2008;27:113-22.
2. Rehman MU, Buttar QM, Khawaja MI, Khawaja MR. An impending cancer crisis in developing countries: are we ready for the challenge? Asian Pac J Cancer Prev. 2009;10:719-20.
3. Dai LC, Wang X, Yao X, Lu YL, Ping JL, He JF. Enhanced therapeutic effects of combined chemotherapeutic drugs and midkine antisense oligonucleotides for hepatocellular carcinoma. World J Gastroenterol. 2007;13:1989-94. (Pubitemid 46746387)
4. Drake CG, Antonarakis ES. Update: immunological strategies for prostate cancer. Curr Urol Rep. 2010;11:202-7.
5. Yu JQ, Liu HB, Lei JC, Tan WJ, Hu XM, Zou GL. Antitumor activity of chloroform fraction of Scutellaria barbata and its active constituents. Phytother Res. 2007;21:817-22.
6. Vickers A. Botanical medicines for the treatment of cancer: rationale, overview of current data, and methodological considerations for phase I and II trials. Cancer Invest. 2002;20:1069-79. (Pubitemid 35315943)
7. Sheu ML, Liu SH, Lan KH. Honokiol induces calpain-mediated glucose-regulated protein-94 cleavage and apoptosis in human gastric cancer cells and reduces tumor growth. PLoS One. 2007;2:e1096.
8. Ahn KS, Hahn BS, Kwack K, Lee EB, Kim YS. Platycodin D-induced apoptosis through nuclear factor-κB activation in immortalized keratinocytes. Eur J Pharmacol. 2006;537:1-11.
9. Kim YP, Lee EB, Kim SY, Li D, Ban HS, Lim SS, et al. Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. Planta Med. 2001;67:362-4. (Pubitemid 32588576)
10. Khanal T, Choi JH, Hwang YP, Chung YC, Jeong HG. Saponins isolated from the root of Platycodon grandiflorum protect against acute ethanol-induced hepatotoxicity in mice. Food Chem Toxicol. 2009;47:530-5.
11. Lee KJ, Choi JH, Kim HG, Han EH, Hwang YP, Lee YC, et al. Protective effect of saponins derived from the roots of Platycodon grandiflorum against carbon tetrachloride induced hepatotoxicity in mice. Food Chem Toxicol. 2008;46:1778-85.
12. Xie Y, Sun HX, Li D. Platycodin D is a potent adjuvant of specific cellular and humoral immune responses against recombinant hepatitis B antigen. Vaccine. 2009;27:757-64.
13. Yu JS, Kim AK. Platycodin D induces apoptosis in MCF-7 human breast cancer cells. J Med Food. 2010;13:298-305.
14. Choi JH, Yoo KY, Park OK, Lee CH, Won MH, Hwang IK, et al. Platycodin D and 2″-O-acetyl-polygalacin D2 isolated from Platycodon grandiflorum protect ischemia/reperfusion injury in the gerbil hippocampus. Brain Res. 2009;1279:197-208.
15. Lee H, Bae S, Kim YS, Yoon Y. WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum. Life Sci. 2011;89:388-94.
16. Kim MO, Moon DO, Choi YH, Shin DY, Kang HS, Choi BT, et al. Platycodin D induces apoptosis and decreases telomerase activity in human leukemia cells. Cancer Lett. 2008;261:98-107.
17. Kim MO, Moon DO, Choi YH, Lee JD, Kim ND, Kim GY. Platycodin D induces mitotic arrest in vitro, leading to endoreduplication, inhibition of proliferation and apoptosis in leukemia cells. Int J Cancer. 2008;122:2674-81.
18. Chun J, Joo EJ, Kang M, Kim YS. Platycodin D induces anoikis and caspase-mediated apoptosis via p38 MAPK in AGS human gastric cancer cells. J Cell Biochem. 2013;114:456-70.
19. Pu L, Amoscato AA, Bier ME, Lazo JS. Dual G1 and G2 phase inhibition by a novel, selective Cdc25 inhibitor 6-chloro-7-[corrected](2-morpholin-4- ylethylamino)-quinoline-5,8-dione. J Biol Chem. 2002;277:46877-85. (Pubitemid 36159191)
20. Chao JI, Kuo PC, Hsu TS. Down-regulation of survivin in nitric oxide induced cell growth inhibition and apoptosis of the human lung carcinoma cells. J Biol Chem. 2004;279:20267-76.
21. Wang Y, Ji P, Liu J, Broaddus RR, Xue F, Zhang W. Centrosome-associated regulators of the G(2)/M checkpoint as targets for cancer therapy. Mol Cancer. 2009;8:8.
22. Arnoult D, Parone P, Martinou JC, Antonsson B, Estaquier J, Ameisen JC. Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuli. J Cell Biol. 2002;159:923-9. (Pubitemid 36055747)
23. Wang ZB, Liu YQ, Cui YF. Pathways to caspase activation. Cell Biol Int. 2005;29:489-96.
24. Sun XM, MacFarlane M, Zhuang J, Wolf BB, Green DR, Cohen GM. Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J Biol Chem. 1999;274:5053-60.
25. Hu W, Kavanagh JJ. Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 2003;4:721-9. (Pubitemid 37527207)
26. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999;15:269-90. (Pubitemid 32250377)
27. Igney FH, Krammer PH. Death and anti-death: tumour resistance to apoptosis. Nat Rev Cancer. 2002;2:277-88. (Pubitemid 37328781)
28. Walczak H, Haas TL. Biochemical analysis of the native TRAIL death inducing signaling complex. In: Mor G, Alvero AB, editors. Methods in molecular biology, apoptosis and cancer. Totowa: Humana; 1998.
29. Karlsson J, Øra I, Pörn-Ares I, Pahlman D. Arsenic trioxide-induced death of neuroblastoma cells involves activation of Bax and does not require p53. Clin Cancer Res. 2004;10:3179-88. (Pubitemid 38619699)
30. Kauffmann-Zeh A, Rodriguez-Viciana P, Ulrich E, Gilbert C, Coffer P, Downward J, et al. Suppression of c-Myc-induced apoptosis by Ras signalling through PI(3)K and PKB. Nature. 1997;385:544-8. (Pubitemid 27074674)
31. Vivanco I, Sawyers CL. The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat Rev Cancer. 2002;2:489-501. (Pubitemid 37328931)
32. Bunz F. Cell death and cancer therapy. Curr Opin Pharmacol. 2001;1:337-41. (Pubitemid 33585981)
33. Taraphdar AK, Roy M, Bhattacharya RK. Natural products as inducers of apoptosis: implication for cancer therapy and prevention. Curr Sci. 2001;80:1387-96. (Pubitemid 33620231)
34. Hengartner MO. The biochemistry of apoptosis. Nature. 2000;407:770-6.
35. Hsu YL, Kuo PL, Lin CC. Acacetin inhibits the proliferation of Hep G2 by blocking cell cycle progression and inducing apoptosis. Biochem Pharmacol. 2004;67:823-9.