The effect of disulfide bond introduction and related Cys/Ser mutations on the stability of a cyclohexanone monooxygenase

Journal of Biotechnology

Volumn 214, Issue , 2015, Pages 199-211

  Schmidt, Sandy ^a   Genz, Maika ^a   Balke, Kathleen ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Computational design; Cyclohexanone monooxygenase; Disulfide bonds; Long term stability; Oxidative stability; Thermostability

Indexed keywords

COVALENT BONDS; ENZYMES; ESTERS; KETONES; SCAFFOLDS; SULFUR COMPOUNDS;

COMPUTATIONAL DESIGN; CYCLOHEXANONE MONOOXYGENASE; DISULFIDE BONDS; LONG TERM STABILITY; OXIDATIVE STABILITY; THERMOSTABILITY;

STABILITY;

BACTERIAL ENZYME; CYCLOHEXANONE MONOOXYGENASE; CYSTEINE; SERINE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYCLOHEXANONE OXYGENASE; DISULFIDE; OXYGENASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL MUTATION; BACTERIAL STRAIN; CATALYTIC EFFICIENCY; CIRCULAR DICHROISM; CONTROLLED STUDY; DISULFIDE BOND; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IN VITRO STUDY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY; TEMPERATURE; ACINETOBACTER; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MUTATION; PROTEIN ENGINEERING;

ACINETOBACTER; BACTERIAL PROTEINS; CYSTEINE; DISULFIDES; ENZYME STABILITY; ESCHERICHIA COLI; MUTATION; OXYGENASES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERINE;

EID: 84944882705     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.09.026     Document Type: Article

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