Directed evolution of phenylacetone monooxygenase as an active catalyst for the baeyer-villiger conversion of cyclohexanone to caprolactone

Biotechnology and Bioengineering

Volumn 112, Issue 7, 2015, Pages 1354-1364

  Parra, Loreto P ^a,b,c   Acevedo, Juan P ^a,b,d   Reetz, Manfred T ^a,b  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^d UNIVERSIDAD DE LOS ANDES   (Chile)

Author keywords

Baeyer Villiger monooxygenase; Cyclohexanone; Directed evolution; E open caprolactone; Literative saturation mutagenesis; Phenylacetone monooxygenase

Indexed keywords

ECONOMIC AND SOCIAL EFFECTS; ESCHERICHIA COLI; MOLECULAR DYNAMICS; MUTAGENESIS;

BAEYER-VILLIGER MONOOXYGENASES; CAPROLACTONE; CYCLOHEXANONES; DIRECTED EVOLUTION; MONOOXYGENASES; SATURATION MUTAGENESIS;

REACTION KINETICS;

CYCLOHEXANONE; PHENYLACETONE MONOOXYGENASE; POLYCAPROLACTONE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; 1-PHENYL-2-PROPANONE; ACETONE; CAPROLACTONE; CYCLOHEXANONE DERIVATIVE; HEXANOIC ACID DERIVATIVE; LACTONE; MIXED FUNCTION OXIDASE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BAEYER VILLIGER REACTION; CATALYSIS; CATALYST; DNA SEQUENCE; ENZYME ACTIVITY; GENETIC SCREENING; HYDROGEN BOND; ITERATIVE SATURATION MUTAGENESIS; MOLECULAR DYNAMICS; MUTAGENESIS; NUCLEOPHILICITY; REACTION TIME; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; ANALOGS AND DERIVATIVES; BIOTRANSFORMATION; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETICS; KINETICS; METABOLISM;

ACETONE; BIOTRANSFORMATION; CAPROATES; CYCLOHEXANONES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; KINETICS; LACTONES; MIXED FUNCTION OXYGENASES; MOLECULAR DYNAMICS SIMULATION; MUTANT PROTEINS; NADP;

EID: 84929956451     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25564     Document Type: Article

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