Development of thermostable Candida antarctica lipase B through novel in silico design of disulfide bridge

Biotechnology and Bioengineering

Volumn 109, Issue 4, 2012, Pages 867-876

  Le, Quang Anh Tuan ^a   Joo, Jeong Chan ^a,b   Yoo, Young Je ^b   Kim, Yong Hwan ^a  

^a Kwangwoon University   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

B factor of residue pairs; Candida antarctica lipase b; Disulfide bridge; Disulfide by design; Flexibility of residue pairs; MODIP; Thermal stability

Indexed keywords

B-FACTOR OF RESIDUE PAIRS; CANDIDA ANTARCTICA LIPASE B; DISULFIDE BRIDGE; FLEXIBILITY OF RESIDUE PAIRS; MODIP;

AMINO ACIDS; COVALENT BONDS; ISOMERS; MOLECULAR DYNAMICS; THERMODYNAMIC STABILITY;

FACTOR ANALYSIS;

CYSTEINE; LIPASE B;

AMINO ACID SEQUENCE; ARTICLE; CANDIDA ANTARCTICA; CATALYSIS; COMPUTER MODEL; CONTROLLED STUDY; DISULFIDE BOND; HALF LIFE TIME; INCUBATION TIME; MOLECULAR DYNAMICS; NONHUMAN; PREDICTOR VARIABLE; PROTEIN BINDING; PROTEIN STABILITY; TEMPERATURE DEPENDENCE; THERMOSTABILITY; WILD TYPE;

AMINO ACID SUBSTITUTION; CANDIDA; CYSTINE; DRUG DESIGN; FUNGAL PROTEINS; GENES, SYNTHETIC; HOT TEMPERATURE; KINETICS; LIPASE; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; PICHIA; PROTEIN CONFORMATION; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; TRANSITION TEMPERATURE;

CANDIDA ANTARCTICA;

EID: 84857440017     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24371     Document Type: Article

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