Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase

Protein Engineering

Volumn 16, Issue 6, 2003, Pages 423-428

  Miyazaki Imamura, Chie ^a   Oohira, Kazuyo ^b   Kitagawa, Ritsuko ^a   Nakano, Hideo ^b   Yamane, Tsuneo ^b   Takahashi, Haruo ^a  

^a Rd Management Section   (Japan)

^b NAGOYA UNIVERSITY   (Japan)

Author keywords

Cell free protein synthesis; High throughput screening; Hydrogen peroxide; Manganese peroxidase; Protein disulfide isomerase

Indexed keywords

HEMIN; HEMOPROTEIN; HYDROGEN PEROXIDE; MANGANESE PEROXIDASE; PROTEIN DISULFIDE ISOMERASE; AMINO ACID; CHAPERONE; PEROXIDASE;

ARTICLE; COMBINATORIAL CHEMISTRY; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; PHANEROCHAETE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETIC TRANSCRIPTION; GENETICS; IN VITRO STUDY; METABOLISM; METHODOLOGY; PHARMACEUTICS; POLYMERASE CHAIN REACTION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

CHRYSOSPORIUM; ESCHERICHIA COLI; PHANEROCHAETE; PHANEROCHAETE CHRYSOSPORIUM;

AMINO ACIDS; ESCHERICHIA COLI; HYDROGEN PEROXIDE; MOLECULAR CHAPERONES; MUTAGENESIS, SITE-DIRECTED; PEROXIDASES; PHANEROCHAETE; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; PROTEIN DISULFIDE-ISOMERASE; SACCHAROMYCES CEREVISIAE; TECHNOLOGY, PHARMACEUTICAL; TRANSCRIPTION, GENETIC;

EID: 0042266271     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzg054     Document Type: Article

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