Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes

BMC Microbiology

Volumn 15, Issue 1, 2015, Pages

  Nagpal, Ravinder ^a   Ogata, Kiyohito ^b   Tsuji, Hirokazu ^b   Matsuda, Kazunori ^c   Takahashi, Takuya ^b   Nomoto, Koji ^b   Suzuki, Yoshio ^d   Kawashima, Kazunari ^e   Nagata, Satoru ^f   Yamashiro, Yuichiro ^a  

^a JUNTENDO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

^c Yakult Honsha European Research Center for Microbiology   (Belgium)

^d JUNTENDO UNIVERSITY   (Japan)

^e Gonohashi Obstetrics and Gynecology Hospital   (Japan)

^f TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

Alpha toxin; Clostridium perfringens; Enterotoxin; Gut pathogens; Intestinal microbiota; Phospholipase C; Quantitative PCR

Indexed keywords

ALPHA TOXIN; ENTEROTOXIN; ALPHA TOXIN, CLOSTRIDIUM PERFRINGENS; BACTERIAL TOXIN; CALCIUM BINDING PROTEIN; ENTEROTOXIN, CLOSTRIDIUM; PHOSPHOLIPASE C;

ADULT; AGE DISTRIBUTION; ARTICLE; BACTERIAL COUNT; BACTERIUM DETECTION; CLOSTRIDIUM PERFRINGENS; FECES ANALYSIS; FEMALE; GENE TARGETING; HUMAN; INFANT; LIMIT OF DETECTION; MALE; NONHUMAN; PREVALENCE; QUANTITATIVE ANALYSIS; QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; YOUNG ADULT; ADOLESCENT; BACTERIAL LOAD; CLOSTRIDIUM INFECTION; COHORT ANALYSIS; FECES; GENETICS; HETEROZYGOTE; ISOLATION AND PURIFICATION; MICROBIOLOGY; MIDDLE AGED; NORMAL HUMAN; PROCEDURES; VALIDATION STUDY;

ADOLESCENT; ADULT; BACTERIAL LOAD; BACTERIAL TOXINS; CALCIUM-BINDING PROTEINS; CARRIER STATE; CLOSTRIDIUM INFECTIONS; CLOSTRIDIUM PERFRINGENS; COHORT STUDIES; ENTEROTOXINS; FECES; FEMALE; HEALTHY VOLUNTEERS; HUMANS; INFANT; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TYPE C PHOSPHOLIPASES; YOUNG ADULT;

EID: 84944385388     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-015-0561-y     Document Type: Article

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