Prevalence of enterotoxigenic Clostridium perfringens isolates in Pittsburgh (Pennsylvania) area soils and home kitchens

Applied and Environmental Microbiology

Volumn 73, Issue 22, 2007, Pages 7218-7224

  Li, Jihong ^a   Sayeed, Sameera ^a   McClane, Bruce A ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASES; GENES; MICROBIOLOGY; SOILS;

FOOD POISONING; GASTROINTESTINAL DISEASES; HOME KITCHENS; SOIL ISOLATES;

BACTERIA;

BACTERIUM; CHROMOSOME; FOOD CHAIN; FOOD POISONING;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CLOSTRIDIUM PERFRINGENS; CPE GENE; ENTEROTOXIGENIC CLOSTRIDIUM PERFRINGENS; FOOD CONTAMINATION; FOOD POISONING; GENOTYPE; KITCHEN; NONHUMAN; PLASMID; SOIL ANALYSIS; SPOROGENESIS; UNITED STATES;

BLOTTING, SOUTHERN; BLOTTING, WESTERN; CLOSTRIDIUM PERFRINGENS; COOKING AND EATING UTENSILS; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROTOXINS; FOOD MICROBIOLOGY; GENOTYPE; HUMANS; PENNSYLVANIA; POLYMERASE CHAIN REACTION; SOIL MICROBIOLOGY;

EURASIA; EUROPE; NORTH AMERICA; PENNSYLVANIA; PITTSBURGH; UNITED STATES;

CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM PERFRINGENS A;

EID: 36649015385     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01075-07     Document Type: Article

References (25)

1. Billington, S. J., E. U. Wieckowski, M. R. Sarker, D. Bueschel, J. G. Songer, and B. A. McClane. 1998. Clostridium perfringens type E animal enteritis isolates with highly conserved, silent enterotoxin sequences. Infect. Immun. 66:4531-4536.
2. Collie, R. E., and B. A. McClane. 1998. Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with nonfood-borne human gastrointestinal diseases. J. Clin. Microbiol. 36:30-36.
3. Cornillot, E., B. Saint-Joanis, G. Daube, S. Katayama, P. E. Granum, B. Carnard, and S. T. Cole. 1995. The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne. Mol. Microbiol. 15:639-647.
4. Fisher, D. J., K. Miyamoto, B. Harrision, S. Akimoto, M. R. Sarker, and B. A. McClane. 2005. Association of beta2 toxin production with Clostridium perfringens type A human gastrointestinal disease isolates carrying a plasmid enterotoxin gene. Mol. Microbiol. 56:747-762.
5. Garmory, H. S., N. Chanter, N. P. French, D. Busechel, J. G. Songer, and R. W. Titball. 2000. Occurence of Clostridium perfringens β2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol. Infect. 124:61-67.
6. Heikinheimo, A., M. Lindstrom, P. E. Granum, and H. Korkeala. 2006. Humans as reservoir for enterotoxin gene-carrying Clostridium perfringens type A. Emerg. Infect. Dis. 12:1724-1729.
7. Kokai-Kun, J. F., J. G. Songer, J. R. Czeczulin, F. Chen, and B. A. McClane. 1994. Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens. J. Clin. Microbiol. 32:2533-2539.
8. Kuske, C. R., S. M. Barns, C. C. Grow, L. Merrill, and J. Dunbar. 2006. Environmental survey for four pathogenic bacteria and closely related species using phylogenetic and functional genes. J. Forensic Sci. 51:548-558.
9. Li, J., and B. A. McClane. 2006. Comparative effects of osmotic, sodium nitrite-induced, and pH-induced stress on growth and survival of Clostridium perfringens type A isolates carrying chromosomal or plasmid-borne enterotoxin genes. Appl. Environ. Microbiol. 72:7620-7625.
10. Li, J., and B. A. McClane. 2006. Further comparison of temperature effects on growth and survival of Clostridium perfringens type A isolates carrying a chromosomal or plasmid-borne enterotoxin gene. Appl. Environ. Microbiol. 72:4561-4568.
11. Matches, J. R., J. Liston, and D. Curran. 1974. Clostridium perfringens in the environment. Appl. Microbiol. 28:655-660.
12. McClane, B. A. 2007. Clostridium perfringens, p. 423-444. In M. P. Doyle and L. R. Beuchat (ed.), Food microbiology, 3rd ed. ASM Press, Washington, DC.
13. McClane, B. A., F. A. Uzal, M. F. Miyakawa, D. Lyerly, and T. Wilkins. 2006. The enterotoxic Clostridia, p. 698-752. In M. Dworkin, S. Falkow, E. Rosenburg, K. H. Schleifer, and E. Stackebrandt (ed.), The prokaryotes, vol. 4. Springer-Verlag, New York, NY.
14. Miyamoto, K., G. Chakrabarti, Y. Morino, and B. A. McClane. 2002. Organization of the plasmid cpe locus of Clostridium perfringens type A isolates. Infect. Immun. 70:4261-4272.
15. Miyamoto, K., D. J. Fisher, J. Li, S. Sayeed, S. Akimoto, and B. A. McClane. 2006. Complete sequencing and diversity analysis of the enterotoxin-encoding plasmids in Clostridium perfringens type A non-food-borne human gastrointestinal disease isolates. J. Bacteriol. 188:1585-1598.
16. Miyamoto, K., Q. Wen, and B. A. McClane. 2004. Multiplex PCR genotyping assay that distinguishes between isolates of Clostridium perfringens type A carrying a chromosomal enterotoxin gene (cpe) locus, a plasmid cpe locus with an IS1470-like sequence or a plasmid cpe locus with an IS1151 sequence. J. Clin. Microbiol. 42:1552-1558.
17. Pedroso, D. M. M., S. T. Iaria, R. C. G. Gamba, S. Heidtmann, and V. L. M. Rall. 1999. Critical control points for meat balls and kibbe preparations in a hospital kitchen. Rev. Microbiol. 30:347-355.
18. Petit, L., M. Gilbert, and M. R. Popoff. 1999. Clostridium perfringens: toxinotype and genotype. Trends Microbiol. 7:104-110.
19. Rood, J. I. 1998. Virulence genes of Clostridium perfringens. Annu. Rev. Microbiol. 52:333-360.
20. Sarker, M. R., R. P. Shivers, S. G. Sparks, V. K. Juneja, and B. A. McClane. 2000. Comparative experiments to examine the effects of heating on vegetative cells and spores of Clostridium perfringens isolates carrying plasmid versus chromosomal enterotoxin genes. Appl. Environ. Microbiol. 66:3234-3240.
21. Stringer, M. F. 1985. Clostridium perfringens type A food poisoning, p. 118-143. In S. P. Borriello (ed.), Clostridia in gastrointestinal disease. CRC Press, Boca Raton, FL.
22. Tanaka, D., K. Kimata, M. Shimizu, J. Isobe, M. Watahiki, T. Karasawa, T. Yamagishi, S. Kuramoto, T. Serikawa, F. Ishiguro, M. Yamada, K. Yamaoka, M. Tokoro, T. Fukao, M. Matsumoto, R. Hiramatsu, C. Monma, and Y. Nagai. 2007. Genotyping of Clostridium perfringens isolates collected from food poisoning outbreaks and healthy individuals in Japan based on the cpe locus. Jpn. J. Infect. Dis. 60:68-69.
23. Taylor, A. W., and W. S. Gordon. 1940. A survey of the types of Cl. welchii present in soil and in the intestinal contents of animals and man. J. Pathol. Bacteriol. 50:271-277.
24. Wen, Q., and B. A. McClane. 2004. Detection of enterotoxigenic Clostridium perfringens type A isolates in American retail foods. Appl. Environ. Microbiol. 70:2685-2691.
25. Wen, Q., K. Miyamoto, and B. A. McClane. 2003. Development of a duplex PCR genotyping assay for distinguishing Clostridium perfringens type A isolates carrying chromosomal enterotoxin (cpe) genes from those carrying plasmid-borne enterotoxin (cpe) genes. J. Clin. Microbiol. 41:1494-1498.