Development of TaqMan-based quantitative PCR for sensitive and selective detection of toxigenic clostridium difficile in human stools

PLoS ONE

Volumn 9, Issue 10, 2014, Pages

  Kubota, Hiroyuki ^a,b   Sakai, Takafumi ^b   Gawad, Agata ^a   Makino, Hiroshi ^b   Akiyama, Takuya ^a,b   Ishikawa, Eiji ^b   Oishi, Kenji ^a,b  

^a Yakult Honsha European Research Center for Microbiology ESV   (Belgium)

^b YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AGED; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CLOSTRIDIUM; CLOSTRIDIUM BIFERMENTANS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM HISTOLYTICUM; CLOSTRIDIUM INNOCUUM; CLOSTRIDIUM NOVYI; CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM RAMOSUM; CLOSTRIDIUM SEPTICUM; CLOSTRIDIUM SORDELLII; CLOSTRIDIUM SPHENOIDES; CLOSTRIDIUM TERTIUM; CONTROLLED STUDY; FECES ANALYSIS; FEMALE; GENE SEQUENCE; GENE TARGETING; HOME FOR THE AGED; LIMIT OF DETECTION; MALE; MEASUREMENT ACCURACY; NONHUMAN; NUCLEOTIDE SEQUENCE; QUALITATIVE VALIDITY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TAQMAN BASED QUANTITATIVE POLYMERASE CHAIN REACTION; BACTERIAL GENE; FECES; GENETICS; HUMAN; IMMUNOASSAY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; NURSING HOME; PEPTOCLOSTRIDIUM DIFFICILE; PROCEDURES; VERY ELDERLY;

CLOSTRIDIUM DIFFICILE;

PRIMER DNA; RNA 16S; TAQ POLYMERASE;

AGED; AGED, 80 AND OVER; CLOSTRIDIUM DIFFICILE; DNA PRIMERS; FECES; FEMALE; GENES, BACTERIAL; HUMANS; IMMUNOASSAY; LIMIT OF DETECTION; MALE; NURSING HOMES; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TAQ POLYMERASE;

EID: 84910020648     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0111684     Document Type: Article

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