Establishment of an analytical system for the human fecal microbiota, based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules

Applied and Environmental Microbiology

Volumn 75, Issue 7, 2009, Pages 1961-1969

  Matsuda, Kazunori ^a   Tsuji, Hirokazu ^a   Asahara, Takashi ^a   Matsumoto, Kazumasa ^a   Takada, Toshihiko ^a   Nomoto, Koji ^a  

^a YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA GENE SEQUENCES; ANALYTICAL SYSTEMS; BACTERIAL POPULATIONS; BACTEROIDES FRAGILIS; BIFIDOBACTERIUM; CLOSTRIDIUM COCCOIDES; CLOSTRIDIUM PERFRINGENS; CULTURE METHODS; ENTEROBACTERIACEAE; ENTEROCOCCUS SPP; FECAL SAMPLES; GROUP-SPECIFIC PRIMERS; HUMAN FECAL; HUMAN INTESTINES; LACTOBACILLUS SPP; LOWER DETECTION LIMITS; MICROBIOTA; PREVOTELLA; PSEUDOMONAS SPP; QUANTITATIVE ANALYSIS; QUANTITATIVE PCR; REVERSE TRANSCRIPTIONS; SPECIFIC PRIMERS;

BACTERIOLOGY; CELL CULTURE; COSMIC RAYS; TRANSCRIPTION;

POPULATION STATISTICS;

RNA 16S;

DETECTION METHOD; DOMINANCE; FECAL COLIFORM; FECES; GENE EXPRESSION; GENETIC ANALYSIS; HOMINID; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; RNA;

ADULT; AGED; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTEROIDES FRAGILIS; BIFIDOBACTERIUM; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; ENTEROBACTERIACEAE; ENTEROCOCCUS; FECES; FECES MICROFLORA; FEMALE; GENE SEQUENCE; HUMAN; HUMAN EXPERIMENT; INCIDENCE; INTESTINE FLORA; LACTOBACILLUS; MALE; MICROBIAL COMMUNITY; NONHUMAN; NORMAL HUMAN; PREVOTELLA; PSEUDOMONAS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA GENE; STAPHYLOCOCCUS;

BACTERIA; COLONY COUNT, MICROBIAL; DNA PRIMERS; FECES; HUMAN EXPERIMENTATION; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY;

ATOPOBIUM; BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS; BIFIDOBACTERIUM; CLOSTRIDIUM; CLOSTRIDIUM COCCOIDES; CLOSTRIDIUM PERFRINGENS; ENTEROBACTERIACEAE; ENTEROCOCCUS; LACTOBACILLUS; PREVOTELLA; PSEUDOMONAS; STAPHYLOCOCCUS;

EID: 63849243189     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01843-08     Document Type: Article

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