Sensitive quantification of Clostridium difficile cells by reverse transcription-quantitative PCR targeting rRNA molecules

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5111-5118

  Matsuda, Kazunori ^a   Tsuji, Hirokazu ^a   Asahara, Takash ^a   Takahashi, Takuya ^a   Kubota, Hiroyuki ^a,b   Nagata, Satoru ^c   Yamashiro, Yuichiro ^c   Nomoto, Koji ^a  

^a YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

^b Yakult Honsha European Research Center for Microbiology   (Belgium)

^c JUNTENDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM DIFFICILE; DETECTION RATES; EFFECTIVE TOOL; HUMAN FECES; HUMAN INTESTINE; LOWER DETECTION LIMIT; POPULATION LEVELS; RRNA GENES; VEGETATIVE CELLS;

CLOSTRIDIUM; RNA; TRANSCRIPTION;

POLYMERASE CHAIN REACTION;

PRIMER DNA; RNA 23S;

BACTERIUM; CELL ORGANELLE; FECES; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; POPULATION STRUCTURE; QUANTITATIVE ANALYSIS; RNA; SENSITIVITY ANALYSIS;

ADULT; AGED; ARTICLE; BACTERIAL COUNT; CLOSTRIDIUM DIFFICILE; COMPARATIVE STUDY; EVALUATION; FECES; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; INTESTINE; JAPAN; METHODOLOGY; MICROBIOLOGY; PSEUDOMEMBRANOUS COLITIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; AGED, 80 AND OVER; CLOSTRIDIUM DIFFICILE; COLONY COUNT, MICROBIAL; DNA PRIMERS; ENTEROCOLITIS, PSEUDOMEMBRANOUS; FECES; HUMANS; INTESTINES; JAPAN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 23S;

CLOSTRIDIUM DIFFICILE;

EID: 84866180610     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07990-11     Document Type: Article

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