메뉴 건너뛰기




Volumn 6, Issue 27, 2015, Pages 23930-23943

E6 and E7 gene silencing results in decreased methylation of tumor suppressor genes and induces phenotype transformation of human cervical carcinoma cell lines

Author keywords

Cell viability and apoptosis; DNA methylation; HPV16 E6 and E7; Human cervical carcinoma cell lines; Immunoblot

Indexed keywords

DNA METHYLTRANSFERASE; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE 3A; DNA METHYLTRANSFERASE 3B; MESSENGER RNA; OSTEONECTIN; PROTEIN E6; PROTEIN E7; SECRETED FRIZZLED RELATED PROTEIN 1; SHORT HAIRPIN RNA; DNA (CYTOSINE 5) METHYLTRANSFERASE; E6 PROTEIN, HUMAN PAPILLOMAVIRUS TYPE 16; ONCOGENE PROTEIN E7, HUMAN PAPILLOMAVIRUS TYPE 16; ONCOPROTEIN; REPRESSOR PROTEIN; SMALL INTERFERING RNA;

EID: 84943384070     PISSN: None     EISSN: 19492553     Source Type: Journal    
DOI: 10.18632/oncotarget.4525     Document Type: Article
Times cited : (24)

References (61)
  • 1
    • 84924271853 scopus 로고    scopus 로고
    • Global cancer statistics, 2012
    • Torre, L.A., et al., Global cancer statistics, 2012. CA Cancer J Clin, 2015. 65:p. 87-108.
    • (2015) CA Cancer J Clin , vol.65 , pp. 87-108
    • Torre, L.A.1
  • 2
    • 14944385553 scopus 로고    scopus 로고
    • Global cancer statistics, 2002
    • Parkin, D.M., et al., Global cancer statistics, 2002. CA Cancer J Clin, 2005. 55:p. 74-108.
    • (2005) CA Cancer J Clin , vol.55 , pp. 74-108
    • Parkin, D.M.1
  • 3
    • 84875997721 scopus 로고    scopus 로고
    • Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes
    • Zhou, J., et al., Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes. Antiviral Research, 2013. 98:p. 305-313.
    • (2013) Antiviral Research , vol.98 , pp. 305-313
    • Zhou, J.1
  • 4
    • 65349107653 scopus 로고    scopus 로고
    • E6 and E7 Gene Silencing and Transformed Phenotype of Human Papillomavirus 16-Positive Oropharyngeal Cancer Cells
    • Rampias, T., et al., E6 and E7 Gene Silencing and Transformed Phenotype of Human Papillomavirus 16-Positive Oropharyngeal Cancer Cells. JNCI Journal of the National Cancer Institute, 2009. 101:p. 412-423.
    • (2009) JNCI Journal of the National Cancer Institute , vol.101 , pp. 412-423
    • Rampias, T.1
  • 5
    • 84856010588 scopus 로고    scopus 로고
    • Transcriptional gene silencing of HPV16 E6/E7 induces growth inhibition via apoptosis in vitro and in vivo
    • Zhou, J., et al., Transcriptional gene silencing of HPV16 E6/E7 induces growth inhibition via apoptosis in vitro and in vivo. Gynecologic Oncology, 2012. 124:p. 296-302.
    • (2012) Gynecologic Oncology , vol.124 , pp. 296-302
    • Zhou, J.1
  • 6
    • 0041967157 scopus 로고    scopus 로고
    • Human papillomaviruses: targeting differentiating epithelial cells for malignant transformation
    • Fehrmann, F. and L.A. Laimins, Human papillomaviruses: targeting differentiating epithelial cells for malignant transformation. Oncogene, 2003. 22:p. 5201-7.
    • (2003) Oncogene , vol.22 , pp. 5201-5207
    • Fehrmann, F.1    Laimins, L.A.2
  • 7
    • 0037434411 scopus 로고    scopus 로고
    • Human papillomavirus types in invasive cervical cancer worldwide:a meta-analysis
    • Clifford, G.M., et al., Human papillomavirus types in invasive cervical cancer worldwide:a meta-analysis. Br J Cancer, 2003. 88:p. 63-73.
    • (2003) Br J Cancer , vol.88 , pp. 63-73
    • Clifford, G.M.1
  • 8
    • 38049040436 scopus 로고    scopus 로고
    • Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China
    • Hong, D., et al., Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China. Int J Gynecol Cancer, 2008. 18:p. 104-9.
    • (2008) Int J Gynecol Cancer , vol.18 , pp. 104-109
    • Hong, D.1
  • 9
    • 0036559896 scopus 로고    scopus 로고
    • Papillomaviruses and cancer:from basic studies to clinical application
    • Zur, H.H., Papillomaviruses and cancer:from basic studies to clinical application. Nat Rev Cancer, 2002. 2:p. 342-50.
    • (2002) Nat Rev Cancer , vol.2 , pp. 342-350
    • Zur, H.H.1
  • 10
    • 0035803583 scopus 로고    scopus 로고
    • Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
    • Elbashir, S.M., et al., Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J, 2001. 20:p. 6877-88.
    • (2001) EMBO J , vol.20 , pp. 6877-6888
    • Elbashir, S.M.1
  • 11
    • 13444262170 scopus 로고    scopus 로고
    • Inhibition of respiratory viruses by nasally administered siRNA
    • Bitko, V., et al., Inhibition of respiratory viruses by nasally administered siRNA. Nat Med, 2005. 11:p. 50-5.
    • (2005) Nat Med , vol.11 , pp. 50-55
    • Bitko, V.1
  • 12
    • 9144272819 scopus 로고    scopus 로고
    • Using RNAi to catch Drosophila genes in a web of interactions:insights into cancer research
    • Dasgupta, R. and N. Perrimon, Using RNAi to catch Drosophila genes in a web of interactions:insights into cancer research. Oncogene, 2004. 23:p. 8359-65.
    • (2004) Oncogene , vol.23 , pp. 8359-8365
    • Dasgupta, R.1    Perrimon, N.2
  • 13
    • 4644350944 scopus 로고    scopus 로고
    • Unlocking the potential of the human genome with RNA interference
    • Hannon, G.J. and J.J. Rossi, Unlocking the potential of the human genome with RNA interference. Nature, 2004. 431: p. 371-8.
    • (2004) Nature , vol.431 , pp. 371-378
    • Hannon, G.J.1    Rossi, J.J.2
  • 14
    • 0036839458 scopus 로고    scopus 로고
    • Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference
    • Capodici, J., K. Kariko and D. Weissman, Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference. J Immunol, 2002. 169:p. 5196-201.
    • (2002) J Immunol , vol.169 , pp. 5196-5201
    • Capodici, J.1    Kariko, K.2    Weissman, D.3
  • 15
    • 0037173610 scopus 로고    scopus 로고
    • Modulation of HIV-1 replication by RNA interference
    • Jacque, J.M., K. Triques and M. Stevenson, Modulation of HIV-1 replication by RNA interference. Nature, 2002. 418: p. 435-8.
    • (2002) Nature , vol.418 , pp. 435-438
    • Jacque, J.M.1    Triques, K.2    Stevenson, M.3
  • 16
    • 33751001588 scopus 로고    scopus 로고
    • Silencing of HIV-1 with RNA interference:a multiple shRNA approach
    • ter Brake, O., et al., Silencing of HIV-1 with RNA interference:a multiple shRNA approach. Mol Ther, 2006. 14:p. 883-92.
    • (2006) Mol Ther , vol.14 , pp. 883-892
    • ter Brake, O.1
  • 17
    • 27344445600 scopus 로고    scopus 로고
    • CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection
    • Anderson, J. and R. Akkina, CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection. Retrovirology, 2005. 2:p. 53.
    • (2005) Retrovirology , vol.2 , pp. 53
    • Anderson, J.1    Akkina, R.2
  • 18
    • 39849108343 scopus 로고    scopus 로고
    • Lentiviral vector design for multiple shRNA expression and durable HIV-1 inhibition
    • ter Brake, O., et al., Lentiviral vector design for multiple shRNA expression and durable HIV-1 inhibition. Mol Ther, 2008. 16:p. 557-64.
    • (2008) Mol Ther , vol.16 , pp. 557-564
    • ter Brake, O.1
  • 19
    • 33748081146 scopus 로고    scopus 로고
    • Simultaneous targeting of HCV replication and viral binding with a single lentiviral vector containing multiple RNA interference expression cassettes
    • Henry, S.D., et al., Simultaneous targeting of HCV replication and viral binding with a single lentiviral vector containing multiple RNA interference expression cassettes. Mol Ther, 2006. 14:p. 485-93.
    • (2006) Mol Ther , vol.14 , pp. 485-493
    • Henry, S.D.1
  • 20
    • 22044452139 scopus 로고    scopus 로고
    • Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment
    • Wu, K.L., et al., Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment. Virus Res, 2005. 112:p. 100-7.
    • (2005) Virus Res , vol.112 , pp. 100-107
    • Wu, K.L.1
  • 21
    • 0037026601 scopus 로고    scopus 로고
    • Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference
    • Jiang, M. and J. Milner, Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene, 2002. 21:p. 6041-8.
    • (2002) Oncogene , vol.21 , pp. 6041-6048
    • Jiang, M.1    Milner, J.2
  • 22
    • 0141730323 scopus 로고    scopus 로고
    • siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells
    • Butz, K., et al., siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. Oncogene, 2003. 22:p. 5938-45.
    • (2003) Oncogene , vol.22 , pp. 5938-5945
    • Butz, K.1
  • 23
    • 27544455180 scopus 로고    scopus 로고
    • RNA interference against human papillomavirus oncogenes in cervical cancer cells results in increased sensitivity to cisplatin
    • Putral, L.N., et al., RNA interference against human papillomavirus oncogenes in cervical cancer cells results in increased sensitivity to cisplatin. Mol Pharmacol, 2005. 68: p. 1311-9.
    • (2005) Mol Pharmacol , vol.68 , pp. 1311-1319
    • Putral, L.N.1
  • 24
    • 33749873618 scopus 로고    scopus 로고
    • Inhibition of cervical cancer cell growth in vitro and in vivo with lentiviral-vector delivered short hairpin RNA targeting human papillomavirus E6 and E7 oncogenes
    • Gu, W., et al., Inhibition of cervical cancer cell growth in vitro and in vivo with lentiviral-vector delivered short hairpin RNA targeting human papillomavirus E6 and E7 oncogenes. Cancer Gene Ther, 2006. 13:p. 1023-32.
    • (2006) Cancer Gene Ther , vol.13 , pp. 1023-1032
    • Gu, W.1
  • 25
    • 0348133602 scopus 로고    scopus 로고
    • In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA
    • Yoshinouchi, M., et al., In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA. Mol Ther, 2003. 8:p. 762-8.
    • (2003) Mol Ther , vol.8 , pp. 762-768
    • Yoshinouchi, M.1
  • 26
    • 33749357784 scopus 로고    scopus 로고
    • Intratumor injection of small interfering RNA-targeting human papillomavirus 18 E6 and E7 successfully inhibits the growth of cervical cancer
    • Fujii, T., et al., Intratumor injection of small interfering RNA-targeting human papillomavirus 18 E6 and E7 successfully inhibits the growth of cervical cancer. Int J Oncol, 2006. 29:p. 541-8.
    • (2006) Int J Oncol , vol.29 , pp. 541-548
    • Fujii, T.1
  • 27
    • 55649097304 scopus 로고    scopus 로고
    • Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention in a mouse model of cervical cancer
    • Jonson, A.L., et al., Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention in a mouse model of cervical cancer. Gynecol Oncol, 2008. 111:p. 356-64.
    • (2008) Gynecol Oncol , vol.111 , pp. 356-364
    • Jonson, A.L.1
  • 28
    • 0038576158 scopus 로고    scopus 로고
    • The power and the promise of DNA methylation markers
    • Laird, P.W., The power and the promise of DNA methylation markers. Nat Rev Cancer, 2003. 3:p. 253-66.
    • (2003) Nat Rev Cancer , vol.3 , pp. 253-266
    • Laird, P.W.1
  • 29
    • 34247194882 scopus 로고    scopus 로고
    • Biomarkers for early detection of breast cancer:what, when, and where?
    • Levenson, V.V., Biomarkers for early detection of breast cancer:what, when, and where? Biochim Biophys Acta, 2007. 1770:p. 847-56.
    • (2007) Biochim Biophys Acta , vol.1770 , pp. 847-856
    • Levenson, V.V.1
  • 30
    • 33645461015 scopus 로고    scopus 로고
    • Discovery of novel methylation biomarkers in cervical carcinoma by global demethylation and microarray analysis
    • Sova, P., et al., Discovery of novel methylation biomarkers in cervical carcinoma by global demethylation and microarray analysis. Cancer Epidemiol Biomarkers Prev, 2006. 15:p. 114-23.
    • (2006) Cancer Epidemiol Biomarkers Prev , vol.15 , pp. 114-123
    • Sova, P.1
  • 31
    • 34247849498 scopus 로고    scopus 로고
    • Methylation-sensitive high resolution melting (MS-HRM):a new approach for sensitive and high-throughput assessment of methylation
    • Wojdacz, T.K. and A. Dobrovic, Methylation-sensitive high resolution melting (MS-HRM):a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res, 2007. 35:p. e41.
    • (2007) Nucleic Acids Res , vol.35
    • Wojdacz, T.K.1    Dobrovic, A.2
  • 32
    • 79954526429 scopus 로고    scopus 로고
    • Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation
    • Candiloro, I.L.M., T. Mikeska and A. Dobrovic, Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation. Epigenetics, 2014. 6:p. 500-507.
    • (2014) Epigenetics , vol.6 , pp. 500-507
    • Candiloro, I.L.M.1    Mikeska, T.2    Dobrovic, A.3
  • 33
    • 74249112134 scopus 로고    scopus 로고
    • Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM
    • Kristensen, L.S., et al., Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM. BMC Cancer, 2009. 9:p. 453.
    • (2009) BMC Cancer , vol.9 , pp. 453
    • Kristensen, L.S.1
  • 34
    • 33947158749 scopus 로고    scopus 로고
    • Viral oncoproteins target the DNA methyltransferases
    • Burgers, W.A., et al., Viral oncoproteins target the DNA methyltransferases. Oncogene, 2006. 26:p. 1650-1655.
    • (2006) Oncogene , vol.26 , pp. 1650-1655
    • Burgers, W.A.1
  • 35
    • 84865191715 scopus 로고    scopus 로고
    • Oncogenic human papillomavirus imposes an instructive pattern of DNA methylation changes which parallel the natural history of cervical HPV infection in young women
    • Leonard, S.M., et al., Oncogenic human papillomavirus imposes an instructive pattern of DNA methylation changes which parallel the natural history of cervical HPV infection in young women. Carcinogenesis, 2012. 33:p. 1286-1293.
    • (2012) Carcinogenesis , vol.33 , pp. 1286-1293
    • Leonard, S.M.1
  • 36
    • 78649396546 scopus 로고    scopus 로고
    • HPV-16 E6 upregulation of DNMT1 through repression of tumor suppressor p53
    • Au, Y.C., et al., HPV-16 E6 upregulation of DNMT1 through repression of tumor suppressor p53. Oncol Rep, 2010. 24:p. 1599-604.
    • (2010) Oncol Rep , vol.24 , pp. 1599-1604
    • Au, Y.C.1
  • 37
    • 78751470921 scopus 로고    scopus 로고
    • Structure and function of mammalian DNA methyltransferases
    • Jurkowska, R.Z., T.P. Jurkowski and A. Jeltsch, Structure and function of mammalian DNA methyltransferases. Chembiochem, 2011. 12:p. 206-22.
    • (2011) Chembiochem , vol.12 , pp. 206-222
    • Jurkowska, R.Z.1    Jurkowski, T.P.2    Jeltsch, A.3
  • 38
    • 66349090454 scopus 로고    scopus 로고
    • High-resolution melting analysis (HRMA):more than just sequence variant screening
    • Vossen, R.H., et al., High-resolution melting analysis (HRMA):more than just sequence variant screening. Hum Mutat, 2009. 30:p. 860-6.
    • (2009) Hum Mutat , vol.30 , pp. 860-866
    • Vossen, R.H.1
  • 39
    • 33751251058 scopus 로고    scopus 로고
    • Gene transfer in humans using a conditionally replicating lentiviral vector
    • Levine, B.L., et al., Gene transfer in humans using a conditionally replicating lentiviral vector. Proc Natl Acad Sci U S A, 2006. 103:p. 17372-7.
    • (2006) Proc Natl Acad Sci U S A , vol.103 , pp. 17372-17377
    • Levine, B.L.1
  • 40
    • 20844455902 scopus 로고    scopus 로고
    • Regulatory considerations for novel gene therapy products:a review of the process leading to the first clinical lentiviral vector
    • Manilla, P., et al., Regulatory considerations for novel gene therapy products:a review of the process leading to the first clinical lentiviral vector. Hum Gene Ther, 2005. 16:p. 17-25.
    • (2005) Hum Gene Ther , vol.16 , pp. 17-25
    • Manilla, P.1
  • 41
    • 68149163117 scopus 로고    scopus 로고
    • PCR-based methods for detecting single-locus DNA methylation biomarkers in cancer diagnostics, prognostics, and response to treatment
    • Kristensen, L.S. and L.L. Hansen, PCR-based methods for detecting single-locus DNA methylation biomarkers in cancer diagnostics, prognostics, and response to treatment. Clin Chem, 2009. 55:p. 1471-83.
    • (2009) Clin Chem , vol.55 , pp. 1471-1483
    • Kristensen, L.S.1    Hansen, L.L.2
  • 42
    • 0029843950 scopus 로고    scopus 로고
    • Methylation-specific PCR:a novel PCR assay for methylation status of CpG islands
    • Herman, J.G., et al., Methylation-specific PCR:a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A, 1996. 93:p. 9821-6.
    • (1996) Proc Natl Acad Sci U S A , vol.93 , pp. 9821-9826
    • Herman, J.G.1
  • 43
    • 0033215453 scopus 로고    scopus 로고
    • p15(INK4B) CpG island methylation in primary acute leukemia is heterogeneous and suggests density as a critical factor for transcriptional silencing
    • Cameron, E.E., S.B. Baylin and J.G. Herman, p15(INK4B) CpG island methylation in primary acute leukemia is heterogeneous and suggests density as a critical factor for transcriptional silencing. Blood, 1999. 94:p. 2445-51.
    • (1999) Blood , vol.94 , pp. 2445-2451
    • Cameron, E.E.1    Baylin, S.B.2    Herman, J.G.3
  • 44
    • 42449142344 scopus 로고    scopus 로고
    • Sensitive Melting Analysis after Real Time-Methylation Specific PCR (SMART-MSP):highthroughput and probe-free quantitative DNA methylation detection
    • Kristensen, L.S., et al., Sensitive Melting Analysis after Real Time-Methylation Specific PCR (SMART-MSP):highthroughput and probe-free quantitative DNA methylation detection. Nucleic Acids Res, 2008. 36:p. e42.
    • (2008) Nucleic Acids Res , vol.36
    • Kristensen, L.S.1
  • 45
    • 0036314222 scopus 로고    scopus 로고
    • Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false positives
    • Rand, K., et al., Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false positives. Methods, 2002. 27:p. 114-20.
    • (2002) Methods , vol.27 , pp. 114-120
    • Rand, K.1
  • 46
    • 33745666856 scopus 로고    scopus 로고
    • Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing
    • Shaw, R.J., et al., Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing. Nucleic Acids Res, 2006. 34:p. e78.
    • (2006) Nucleic Acids Res , vol.34
    • Shaw, R.J.1
  • 47
    • 0034654669 scopus 로고    scopus 로고
    • MethyLight:a high-throughput assay to measure DNA methylation
    • Eads, C.A., et al., MethyLight:a high-throughput assay to measure DNA methylation. Nucleic acids research, 2000. 28:p. E32.
    • (2000) Nucleic acids research , vol.28 , pp. E32
    • Eads, C.A.1
  • 48
    • 85018162105 scopus 로고    scopus 로고
    • Association of aberrant DNA methylation with clinicopathological features in breast cancer
    • Tserga, A., Association of aberrant DNA methylation with clinicopathological features in breast cancer. Oncology Reports, 2011.
    • (2011) Oncology Reports
    • Tserga, A.1
  • 49
    • 0035902115 scopus 로고    scopus 로고
    • Proliferation, cell cycle and apoptosis in cancer
    • Evan, G.I. and K.H. Vousden, Proliferation, cell cycle and apoptosis in cancer. Nature, 2001. 411:p. 342-8.
    • (2001) Nature , vol.411 , pp. 342-348
    • Evan, G.I.1    Vousden, K.H.2
  • 50
    • 23044507007 scopus 로고    scopus 로고
    • Short double-stranded RNA induces transcriptional gene silencing in human cancer cells in the absence of DNA methylation
    • Ting, A.H., et al., Short double-stranded RNA induces transcriptional gene silencing in human cancer cells in the absence of DNA methylation. Nat Genet, 2005. 37:p. 906-10.
    • (2005) Nat Genet , vol.37 , pp. 906-910
    • Ting, A.H.1
  • 51
    • 84875997721 scopus 로고    scopus 로고
    • Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes
    • Zhou, J., et al., Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes. Antiviral Res, 2013. 98:p. 305-13.
    • (2013) Antiviral Res , vol.98 , pp. 305-313
    • Zhou, J.1
  • 52
    • 33645151304 scopus 로고    scopus 로고
    • Criteria for effective design, construction, and gene knockdown by shRNA vectors
    • Taxman, D.J., et al., Criteria for effective design, construction, and gene knockdown by shRNA vectors. BMC Biotechnol, 2006. 6:p. 7.
    • (2006) BMC Biotechnol , vol.6 , pp. 7
    • Taxman, D.J.1
  • 53
    • 70449522296 scopus 로고    scopus 로고
    • Gene silencing of HPV16 E6 E7 induced by promoter-targeting siRNA in SiHa cells
    • Hong, D., et al., Gene silencing of HPV16 E6 E7 induced by promoter-targeting siRNA in SiHa cells. British Journal of Cancer, 2009. 101:p. 1798-1804.
    • (2009) British Journal of Cancer , vol.101 , pp. 1798-1804
    • Hong, D.1
  • 54
    • 60849088248 scopus 로고    scopus 로고
    • Inhibition of cervical cancer cell growth by human papillomavirus virus-like particles packaged with human papillomavirus oncoprotein short hairpin RNAs
    • Bousarghin, L., et al., Inhibition of cervical cancer cell growth by human papillomavirus virus-like particles packaged with human papillomavirus oncoprotein short hairpin RNAs. Molecular Cancer Therapeutics, 2009. 8:p. 357-365.
    • (2009) Molecular Cancer Therapeutics , vol.8 , pp. 357-365
    • Bousarghin, L.1
  • 55
    • 84876482702 scopus 로고    scopus 로고
    • Multiple promoters and targeted microRNAs direct the expressions of
    • Li, L., et al., Multiple promoters and targeted microRNAs direct the expressions of. Animal Genetics, 2013. 44(3):p. 241-250.
    • (2013) Animal Genetics , vol.44 , Issue.3 , pp. 241-250
    • Li, L.1
  • 56
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    • Livak, K.J. and T.D. Schmittgen, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001. 25:p. 402-8.
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 57
    • 84877098846 scopus 로고    scopus 로고
    • Methylation of the hTERT Promoter: A Novel Cancer Biomarker for Leptomeningeal Metastasis Detection in Cerebrospinal Fluids
    • Bougel, S., et al., Methylation of the hTERT Promoter: A Novel Cancer Biomarker for Leptomeningeal Metastasis Detection in Cerebrospinal Fluids. Clinical Cancer Research, 2013. 19:p. 2216-2223.
    • (2013) Clinical Cancer Research , vol.19 , pp. 2216-2223
    • Bougel, S.1
  • 58
    • 84872242917 scopus 로고    scopus 로고
    • Comparison study of MS-HRM and pyrosequencing techniques for quantification of APC and CDKN2A gene methylation
    • Migheli, F., et al., Comparison study of MS-HRM and pyrosequencing techniques for quantification of APC and CDKN2A gene methylation. PLoS One, 2013. 8:p. e52501.
    • (2013) PLoS One , vol.8
    • Migheli, F.1
  • 59
    • 84896717678 scopus 로고    scopus 로고
    • Validation of methylation-sensitive highresolution melting (MS-HRM) for the detection of stool DNA methylation in colorectal neoplasms
    • Xiao, Z., et al., Validation of methylation-sensitive highresolution melting (MS-HRM) for the detection of stool DNA methylation in colorectal neoplasms. Clinica Chimica Acta, 2014. 431:p. 154-163.
    • (2014) Clinica Chimica Acta , vol.431 , pp. 154-163
    • Xiao, Z.1
  • 60
    • 0028907552 scopus 로고
    • Functional p53 protein in human papillomavirus-positive cancer cells
    • Butz, K., et al., Functional p53 protein in human papillomavirus-positive cancer cells. Oncogene, 1995. 10: p. 927-36.
    • (1995) Oncogene , vol.10 , pp. 927-936
    • Butz, K.1
  • 61
    • 0141730323 scopus 로고    scopus 로고
    • siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells
    • Butz, K., et al., siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. Oncogene, 2003. 22:p. 5938-45.
    • (2003) Oncogene , vol.22 , pp. 5938-5945
    • Butz, K.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.