Gene silencing of HPV16 E6/E7 induced by promoter-targeting siRNA in SiHa cells

British Journal of Cancer

Volumn 101, Issue 10, 2009, Pages 1798-1804

  Hong, D ^a   Lu, W ^a   Ye, F ^a   Hu, Y ^a   Xie, X ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Histone methylation; Human papillomavirus (HPV); Senescence; Small interfering RNA (siRNA); Transcriptional gene silencing (TGS)

Indexed keywords

HISTONE; HISTONE H3; LYSINE; MESSENGER RNA; SMALL INTERFERING RNA;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL DEATH; CELL GROWTH; CELL LINE; CELL PROLIFERATION; CELL STRAIN; DNA METHYLATION; FEMALE; GENE EXPRESSION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 16; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENESCENCE; UTERINE CERVIX CANCER;

APOPTOSIS; CELL AGING; CELL GROWTH PROCESSES; CELL LINE, TUMOR; FEMALE; GENE EXPRESSION REGULATION, VIRAL; GENE SILENCING; HUMAN PAPILLOMAVIRUS 16; HUMANS; ONCOGENE PROTEINS, VIRAL; PROMOTER REGIONS, GENETIC; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSFECTION; UTERINE CERVICAL NEOPLASMS;

EID: 70449522296     PISSN: 00070920     EISSN: 15321827     Source Type: Journal    
DOI: 10.1038/sj.bjc.6605344     Document Type: Article

References (30)

1. Butz K, Ristriani T, Hengstermann A, Denk C, Scheffner M, Hoppe-Seyler F (2003) SiRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. Oncogene 22: 5938-5945
2. Castanotto D, Tommasi S, Li M, Li H, Yanow S, Pfeifer GP, Rossi JJ (2005) Short hairpin RNA-directed cytosine (CpG) methylation of the RASSF1A gene promoter in HeLa cells. Mol Ther 12: 179-183
3. Daling JR, Madeleine MM, Johnson LG, Schwartz SM, Shera KA, Wurscher MA, Carter JJ, Porter PL, Galloway DA, McDougall JK (2004) Human papillomavirus, smoking, and sexual practices in the etiology of anal cancer. Cancer 101: 270-280
4. Dostatni N, Lambert PF, Sousa R, Ham J, Howley PM, Yaniv M (1991) The functional BPV-1 E2 trans-activating protein can act as a repres-sor by preventing formation of the initiation complex. Genes Dev 5: 1657-1671
5. Dupont JM, Tost J, Jammes H, Gut IG (2004) De novo quantitative bisulfite sequencing using the pyrosequencing technology. Anal Biochem 333: 119-127
6. Goodwin EC, DiMaio D (2000) Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proc Natl Acad Sci USA 97: 12513-12518
7. Grewal SI, Rice JC (2004) Regulation of heterochromatin by histone methylation and small RNAs. Curr Opin Cell Biol 16: 230-238
8. Grunau C, Clark SJ, Rosenthal A (2001) Bisulfite genomic sequencing: systematic investigation of critical experimental parameters. Nucleic Acids Res 29: E65-E65
9. Hall AH, Alexander KA (2003) RNA interference of human papilloma-virus type 18 E6 and E7 induces senescence in HeLa cells. J Virol 77: 6066-6069
10. Hong D, Ye F, Chen H, Lu W, Cheng Q, Hu Y, Xie X (2008) Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China. Int J Gynecol Cancer 18: 104-109
11. Horner SM, DeFilippis RA, Manuelidis L, DiMaio D (2004) Repression of the human papillomavirus E6 gene initiates p53-dependent, telomerase-independent senescence and apoptosis in HeLa cervical carcinoma cells. J Virol 78: 4063-4073 (Pubitemid 38437213)
12. Innocenzi D, Alo PL, Balzani A, Sebastiani V, Silipo V, La Torre G, Ricciardi G, Bosman C, Calvieri S (2003) Fatty acid synthase expression in melanoma. J Cutan Pathol 30: 23-28
13. Jiang M, Milner J (2002) Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene 21: 6041-6048
14. Jones L, Ratcliff F, Baulcombe DC (2001) RNA-directed transcrip-tional gene silencing in plants can be inherited independently of the RNA trigger and requires Met1 for maintenance. Curr Biol 11: 747-757
15. Mantovani F, Banks L (2001) The human papillomavirus E6 protein and its contribution to malignant progression. Oncogene 20: 7874-7887
16. Mette MF, Aufsatz W, van der Winden J, Matzke MA, Matzke AJ (2000) Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J 19: 5194-5201
17. Miller CS, Johnstone BM (2001) Human papillomavirus as a risk factor for oral squamous cell carcinoma: a meta-analysis, 1982-1997. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91: 622-635
18. Morris KV, Chan SW, Jacobsen SE, Looney DJ (2004) Small interfering RNA-induced transcriptional gene silencing in human cells. Science 305: 1289-1292
19. Munger K, Baldwin A, Edwards KM, Hayakawa H, Nguyen CL, Owens M, Grace M, Huh K (2004) Mechanisms of human papillomavirus-induced oncogenesis. J Virol 78: 11451-11460
20. Rajeevan MS, Swan DC, Duncan K, Lee DR, Limor JR, Unger ER (2006) Quantitation of site-specific HPV 16 DNA methylation by pyrosequen-cing. J Virol Methods 138: 170-176
21. Sijen T, Vijn I, Rebocho A, van Blokland R, Roelofs D, Mol JN, Kooter JM (2001) Transcriptional and posttranscriptional gene silencing are mechanistically related. Curr Biol 11: 436-440
22. Smotkin D, Wettstein FO (1986) Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proc Natl Acad Sci USA 83: 4680-4684
23. Sohail M, Doran G, Riedemann J, Macaulay V, Southern EM (2003) A simple and cost-effective method for producing small interfering RNAs with high efficacy. Nucleic Acids Res 31: e38
24. Stunkel W, Huang Z, Tan SH, O'Connor MJ, Bernard HU (2000) Nuclear matrix attachment regions of human papillomavirus type 16 repress or activate the E6 promoter, depending on the physical state of the viral DNA. J Virol 74: 2489-2501
25. Tan SH, Gloss B, Bernard HU (1992) During negative regulation of the human papillomavirus-16 E6 promoter, the viral E2 protein can displace Sp1 from a proximal promoter element. Nucleic Acids Res 20: 251-256
26. Ting AH, Schuebel KE, Herman JG, Baylin SB (2005) Short double-stranded RNA induces transcriptional gene silencing in human cancer cells in the absence of DNA methylation. Nat Genet 37: 906-910
27. Walboomers JM, Jacobs MV, Manos MM, Bosch FX, Kummer JA, Shah KV, Snijders PJ, Peto J, Meijer CJ, Munoz N (1999) Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 189: 12-19
28. Wong HL, Byun HM, Kwan JM, Campan M, Ingles SA, Laird PW, Yang AS (2006) Rapid and quantitative method of allele-specific DNA methylation analysis. Biotechniques 41: 734-739
29. Yoshinouchi M, Yamada T, Kizaki M, Fen J, Koseki T, Ikeda Y, Nishihara T, Yamato K (2003) In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA. Mol Ther 8: 762-768
30. Zhu WG, Srinivasan K, Dai Z, Duan W, Druhan LJ, Ding H, Yee L, Villalona-Calero MA, Plass C, Otterson GA (2003) Methylation of adjacent CpG sites affects Sp1/Sp3 binding and activity in the p21(Cip1) promoter. Mol Cell Biol 23: 4056-4065