Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 18, 1996, Pages 9821-9826

  Herman, James G ^a   Graff, Jeremy R ^a   Myöhänen, Sanna ^a   Nelkin, Barry D ^a   Baylin, Stephen B ^a  

^a DEPARTMENT OF PATHOLOGY   (United States)

Author keywords

DNA methylation; p15; p16; tumor suppressor genes

Indexed keywords

BISULFITE; DINUCLEOTIDE; DNA; UVOMORULIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DNA DETERMINATION; DNA METHYLATION; ENZYME ASSAY; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; TUMOR SUPPRESSOR GENE;

ANTISENSE ELEMENTS (GENETICS); BASE SEQUENCE; CADHERINS; CARRIER PROTEINS; CELL CYCLE PROTEINS; CELL LINE; CPG ISLANDS; CYCLIN-DEPENDENT KINASE INHIBITOR P15; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DNA PRIMERS; GENES, TUMOR SUPPRESSOR; HUMANS; LIGASES; METHYLATION; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEINS; RESTRICTION MAPPING; TUMOR SUPPRESSOR PROTEINS; UBIQUITIN-PROTEIN LIGASES; VON HIPPEL-LINDAU TUMOR SUPPRESSOR PROTEIN;

EID: 0029843950     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.18.9821     Document Type: Article

References (32)

1. Holliday, R. & Grigg, G. W. (1993) Mutat. Res. 285, 61-67.
2. Bird, A. (1992) Cell 70, 5-8.
3. Bird, A. P. (1986) Nature (London) 321, 209-213.
4. Li, E., Beard, C. & Jaenisch, R. (1993) Nature (London) 366, 362-365.
5. Tremblay, K. D., Saam, J. R., Ingram, R. S., Tilghman, S. M. & Bartolomei, M. S. (1995) Nat. Genet. 9, 407-413.
6. Pfeifer, G. P., Steigerwald, S. D., Mueller, P. R., Wold, B. & Riggs, A. D. (1989) Science 246, 810-813.
7. Riggs, A. D. & Pfeifer, G. P. (1992) Trends Genet. 8, 169-174.
8. Antequera, F., Boyes, J. & Bird, A. (1990) Cell 62, 503-514.
9. Herman, J. G., Latif, F., Weng, Y., Lerman, M. I., Zbar, B., Liu, S., Samid, D., Duan, D. S., Gnarra, J. R., Linehan, W. M. & Baylin, S. B. (1994) Proc. Natl. Acad. Sci. USA 91, 9700-9704.
10. Merlo, A., Herman, J. G., Mao, L., Lee, D. J., Gabrielson, E., Burger, P. C., Baylin, S. B. & Sidransky, D. (1995) Nat. Med. 1, 686-692.
11. Herman, J. G., Jen, J., Merlo, A. & Baylin, S. B. (1996) Cancer Res. 56, 722-727.
12. Graff, J. R., Herman, J. G., Lapidus, R. G., Chopra, H., Xu, R., Jarrard, D. F., Isaacs, WB, Pitha, P. M., Davidson, N. E. & Baylin, S. B. (1995) Cancer Res. 55, 5195-5199.
13. Issa, J. P., Ottaviano, Y. L., Celano, P., Hamilton, S. R., Davidson, N. E. & Baylin, S. B. (1994) Nat. Genet. 7, 536-540.
14. Singer-Sam, J., Grant, M., LeBon, J. M., Okuyama, K., Chapman, V., Monk, M. & Riggs, A. D. (1990) Mol. Cell. Biol. 10, 4987-4989.
15. Razin, A. & Cedar, H. (1991) Microbiol. Rev. 55, 451-458.
16. Stoger, R., Kubicka, P., Liu, C. G., Kafri, T., Razin, A., Cedar H. & Barlow, D. P. (1993) Cell 73, 61-71.
17. Frommer, M., McDonald, L. E., Millar, D. S., Collis, C. M. Watt, F., Grigg, G. W., Molloy, P. L. & Paul, C. L. (1992) Proc. Natl. Acad. Sci. USA 89, 1827-1831.
18. Wang, R. Y.-H., Gehrke, C. W. & Ehrlich, M. (1980) Nucleic Acids Res. 8, 4777-4790.
19. Myöhänen, S., Wahlfors, J. & Janne, J. (1994) DNA Sequence 5, 1-8.
20. Hara, E., Smith, R., Parry, D., Tahara, H., Steven, S. & Peters, G. (1996) Mol. Cell. Biol. 16, 859-867.
21. Hussussian, C. J., Struewing, J. P., Goldstein, A. M., Higgins, P. A., Ally, D. S., Sheahan, M. D., Clark, W. H., Jr., Tucker, M. A. & Dracopoli, N. C. (1994) Nat. Genet. 8, 15-21.
22. Jen, J., Harper, J. W., Bigner, S. H., Bigner, D. D., Papadopoulos, N., Markowitz, S., Willson, J. K., Kinzler, K. W. & Vogelstein, B. (1994) Cancer Res. 54, 6353-6358.
23. Kuzmin, I., Duh, F. M., Latif, F., Geil, L., Zbar, B. & Lerman, M. I. (1995) Oncogene 10, 2185-2194.
24. Bussemakers, M. J., Giroldi, L. A., van Bokhoven, A. & Schalken, J. A. (1994) Biochem. Biophys. Res. Commun. 203, 1284-1290.
25. Herman, J. G., Merlo, A., Mao, L., Lapidus, R. G., Issa, J. P. J., Davidson, N. E., Sidransky, D. & Baylin, S. B. (1995) Cancer Res. 55, 4525-4530.
26. Gonzalez-Zulueta, M., Bender, C. M., Yang, A. S., Nguyen, T., Beart, R. W., Van Tornout, J. M. & Jones, P. A. (1995) Cancer Res. 55, 4531-4535.
27. Otterson, G. A., Khleif, S. N., Chen, W., Coxon, A. B. & Kaye, F. J. (1995) Oncogene 11, 1211-1216.
28. Park, J. G. & Chapman, V. M. (1994) Mol. Cell. Biol. 14, 7975-7983.
29. Reeben, M., Myöhänen, S., Saarma, M. & Prydz, H. (1995) Gene 157, 325-329.
30. Gnarra, J. R., Tory, K., Weng, Y., Schmidt, L., Wei, M. H., et al. (1994) Nat. Genet. 7, 85-90.
31. Yoshiura, K., Kanai, Y., Ochiai, A., Shimoyama, Y., Sugimura, T. & Hirohashi, S. (1995) Proc. Natl. Acad. Sci. USA 92, 7416-7419.
32. Clark, S. J., Harrison, J., Paul, C. L. & Frommer, M. (1994) Nucleic Acids Res. 22, 2990-2997.