A Four-Plex Real-Time PCR Assay, Based on rfbE, stx1, stx2, and eae Genes, for the Detection and Quantification of Shiga Toxin-Producing Escherichia coli O157 in Cattle Feces

Foodborne Pathogens and Disease

Volumn 12, Issue 9, 2015, Pages 787-794

  Noll, Lance W ^a   Shridhar, Pragathi B ^a   Shi, Xiaorong ^a   An, Baoyan ^a   Cernicchiaro, Natalia ^a   Renter, David G ^a   Nagaraja, Tiruvoor G ^a   Bai, Jianfa ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; VIRULENCE FACTOR; ADHESIN; AMINOTRANSFERASE; EAEA PROTEIN, E COLI; EPIMERASE; ESCHERICHIA COLI PROTEIN; PEROSAMINE SYNTHETASE; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BOVINE; CHI SQUARE TEST; COLONY FORMING UNIT; EAE GENE; ESCHERICHIA COLI O157; FECES ANALYSIS; GENE TARGETING; LIMIT OF DETECTION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RFBE GENE; SCREENING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STX 2 GENE; STX1 GENE; VALIDATION STUDY; ANIMAL; FECES; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES;

ADHESINS, BACTERIAL; ANIMALS; CARBOHYDRATE EPIMERASES; CATTLE; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; FECES; REAL-TIME POLYMERASE CHAIN REACTION; SHIGA TOXIN 1; SHIGA TOXIN 2; TRANSAMINASES;

EID: 84940656625     PISSN: 15353141     EISSN: 15567125     Source Type: Journal    
DOI: 10.1089/fpd.2015.1951     Document Type: Article

References (43)

1. Anklam KS, Kanankege KS, Gonzales TK, Kaspar CW, Dopfer D. Rapid and reliable detection of Shiga toxin-producing Escherichia coli by real-time multiplex PCR. J Food Prot 2012;75:643-650.
2. Arthur TM, Brichta-Harhay DM, Bosilevac JM, Kalchayanand N, Shackelford SD, Wheeler TL, Koohmaraie M. Super shedding of Escherichia coli O157:H7 by cattle and the impact on beef carcass contamination. Meat Sci 2010;86:32-37.
3. Bai J, Paddock ZD, Shi X, Li S, An B, Nagaraja TG. Applicability of a multiplex PCR to detect the seven major Shiga toxin-producing Escherichia coli based on genes that code for serogroup-specific O-antigens and major virulence factors in cattle feces. Foodborne Pathog Dis 2012;9:541-548.
4. Bai J, Shi X, Nagaraja TG. A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7. J Microbiol Meth 2010;82:85-89.
5. Bertrand R, Roig B. Evaluation of enrichment-free PCR-based detection on the rfbE gene of Escherichia coli O157 - Application to municipal wastewater. Water Res 2007;41:1280-1286.
6. Brichta-Harhay DM, Arthur TM, Bosilevac JM, Guerini MN, Kalchayanand N, Koohmaraie M. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. J Appl Microbiol 2007;103:1657-1668.
7. Burns F, Fleck L, Andaloro B, Davis E, Rohrbeck J, Tice G, Wallace M. DuPont Qualicon BAXO® System Real-Time PCR assay for Escherichia coli O157:H7. J AOAC Int 2011;94:1117-1124.
8. Chapman PA. Methods available for the detection of Escherichia coli O157:H7 in clinical, food, and environmental samples. World J Microbiol Biotechnol 2000;16:733-740.
9. Cull CA, Paddock ZD, Nagaraja TG, Bello NM, Babcock AH, Renter DG. Efficacy of a vaccine and a direct-fed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle. Vaccine 2012;30:6210-6215.
10. Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev 2000;13:559-570.
11. Fegan N, Higgs G, Vanderlinde P, Desmarchelier P. Enumeration of Escherichia coli O157 in cattle faeces using most probable number technique and automated immunomagnetic separation. Lett Appl Microbiol 2004;38:56-59.
12. Fitzmaurice J, Glennon M, Duffy G, Sheridan JJ, Carroll C, Maher M. Application of real-time PCR and RT-PCR assays for the detection and quantitation of VT 1 and VT 2 toxin genes in E. coli O157:H7. Mol Cell Probes 2004;18:123-132.
13. Fortin NY, Mulchandani A, Chen W. Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7. Anal Biochem 2001;289:281-288.
14. Fox JT, Renter DG, Sanderson MW, Nutsch AL, Shi X, Nagaraja TG. Associations between the presence and magnitude of Escherichia coli O157 in feces at harvest and contamination of preintervention beef carcasses. J Food Prot 2008;71:1761-1767.
15. Fox JT, Renter DG, Sanderson MW, Thomson DU, Lechtenberg KF, Nagaraja TG. Evaluation of culture methods to identify bovine feces with high concentrations of Escherichia coli O157. Appl Environ Microbiol 2007;73:5253-5260.
16. Fratamico PM, Wasilenko JL, Garman B, DeMarco DR, Varkey S, Jensen M, Rhoden K, Tice G. Evaluation of a multiplex real-time PCR method for detecting Shiga toxin-producing Escherichia coli in beef and comparison to the US Department of Agriculture Food Safety and Inspection Service microbiology laboratory guidebook method. J Food Prot 2014;77:180-188.
17. Guy RA, Tremblay D, Beausoleil L, Harel J, Champagne MJ. Quantification of E. coli O157 and STEC in feces of farm animals using direct multiplex real time PCR (qPCR) and a modified most probable number assay comprised of immunomagnetic bead separation and qPCR detection. J Microbiol Meth 2014;99:44-53.
18. Gyles CL. Shiga toxin-producing Escherichia coli: An overview. J Anim Sci 2007;85:45-62.
19. Hsu CF, Tsai TY, Pan TM. Use of the duplex TaqMan PCR system for detection of Shiga-like toxin-producing Escherichia coli O157. J Clin Microbiol 2005;43:2668-2673.
20. Ibekwe AM, Grieve CM. Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR. J Appl Microbiol 2003;94:421-431.
21. Jackson MP, Neill RJ, O'Brien AD, Holmes RK, Newland JW. Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933J. FEMS Microbiol Lett 1987;44:109-114.
22. Jacob ME, Bai J, Renter DG, Rogers AT, Shi X, Nagaraja TG. Comparing real-time and conventional PCR to culture-based methods for detecting and quantifying Escherichia coli O157 in cattle feces. J Food Prot 2014;77:314-319.
23. Jacob ME, Renter DG, Nagaraja TG. Animal and truckload-level associations between Escherichia coli O157:H7 in feces and on hides at harvest and contamination of preevisceration beef carcasses. J Food Prot 2010;73:1030-1037.
24. Jacob ME, Shi X, An B, Nagaraja TG, Bai J. Evaluation of a multiplex real-time polymerase chain reaction for the quantification of Escherichia coli O157 in cattle feces. Foodborne Pathog Dis 2012;9:79-85.
25. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics 1977;33:159-174.
26. LeJeune JT, Hancock DD, Besser TE. Sensitivity of Escherichia coli O157 detection in bovine feces assessed by broth enrichment followed by immunomagnetic separation and direct plating methodologies. J Clin Microbiol 2006;44:872-875.
27. Luedtke BE, Bono JL, Bosilevac JM. Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces. J Microbiol Meth 2014;105:72-79.
28. Mackay IM. Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 2004;10:190-212.
29. Madic J, Vingadassalon N, de Garam CP, Marault M, Scheutz F, Brugère H, Jamet E, Auvray F. Detection of Shiga toxin- producing Escherichia coli serotypes O26: H11, O103: H2, O111: H8, O145: H28, and O157: H7 in raw-milk cheeses by using multiplex real-time PCR. Appl Environ Microbiol 2011; 77:2035-2041.
30. Mancusi R, Trevisani M. Enumeration of verocytotoxigenic Escherichia coli (VTEC) O157 and O26 in milk by quantitative PCR. Int J Food Microbiol 2014;184:121-127.
31. Matthews L, McKendrick IJ, Ternent H, Gunn GJ, Synge B, Woolhouse MEJ. Super-shedding cattle and the transmission dynamics of Escherichia coli O157. Epidemiol Infect 2006; 134:131-142.
32. McNemar Q. Note on the sampling error of the difference between correlated proportions or percentages. Psychometrika 1947;12:153-157.
33. Omisakin F, MacRae M, Ogden ID, Strachan NJC. Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Appl Environ Microbiol 2003;69:2444-2447.
34. Pavlovic M, Huber I, Skala H, Konrad R, Schmidt H, Sing A, Busch U. Development of a multiplex real-time polymerase chain reaction for simultaneous detection of enterohemorrhagic Escherichia coli and enteropathogenic Escherichia coli strains. Foodborne Pathog Dis 2010;7:801-808.
35. Rangel JM, Sparling PH, Crowe C, Griffin PM, Swerdlow DL. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002. Emerg Infect Dis 2005;11:603-609.
36. Reischl U, Youssef MT, Kilwinski J, Lehn N, Zhang WL, Karch H, Strockbine NA. Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli. J Clin Microbiol 2002;40:2555-2565.
37. Robinson SE, Wright EJ, Williams NJ, Hart CA, French NP. Development and application of a spiral plating method for the enumeration of Escherichia coli O157 in bovine faeces. J Appl Microbiol 2004;97:581-589.
38. Russo P, Botticella G, Capozzi V, Massa S, Spano G, Beneduce L. A fast, reliable, and sensitive method for detection and quantification of Listeria monocytogenes and Escherichia coli O157: H7 in ready-to-eat fresh-cut products by MPN-qPCR. BioMed Res Int 2014;doi: 10.1155/2014/608296.
39. Sharma VK, Dean-Nystrom EA. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex realtime PCR assay for genes encoding intimin and Shiga toxins. Vet Microbiol 2003;93:247-260.
40. Stanford K, Stephens TP, McAllister TA. Use of model supershedders to define the role of pen floor and hide contamination in the transmission of Escherichia coli O157:H7. J Anim Sci 2010;89:237-244.
41. Stephens TP, Loneragan GH, Chaney WE, Branham LA, Brashears MM. Development and validation of a most-probable-number-immunomagnetic separation methodology of enumerating Escherichia coli O157 in cattle feces. J Food Prot 2007;70:1072-1075.
42. Wang L, Li Y, Mustapha A. Rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella, and Shigella in ground beef by multiplex real-time PCR and immunomagnetic separation. J Food Prot 2007;70:1366-1372.
43. Wasilenko JL, Fratamico PM, Sommers C, DeMarco DR, Varkey S, Rhoden K, Tice G. Detection of Shiga toxin-producing Escherichia coli (STEC) O157: H7, O26, O45, O103, O111, O121, and O145, and Salmonella in retail raw ground beef using the DuPontO™ BAXO® System. Front Cell Infec Microbiol 2014;4:81.