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Volumn 77, Issue 2, 2014, Pages 314-319

Comparing real-time and conventional PCR to culture-based methods for detecting and quantifying Escherichia coli O157 in cattle feces

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; ESCHERICHIA COLI PROTEIN; VEROTOXIN 1; VEROTOXIN 2;

EID: 84893476481     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X.JFP-13-304     Document Type: Article
Times cited : (15)

References (24)
  • 2
    • 77953544892 scopus 로고    scopus 로고
    • A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7
    • Bai, J., X. Shi, and T. G. Nagaraja. 2010. A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7. J. Microbiol. Methods 82:85-89.
    • (2010) J. Microbiol. Methods , vol.82 , pp. 85-89
    • Bai, J.1    Shi, X.2    Nagaraja, T.G.3
  • 3
    • 17644415011 scopus 로고    scopus 로고
    • Rapid detection of diarrheagenic E. Coli by real-time PCR
    • Bischoff, C., J. Luthy, M. Altwegg, and F. Baggi. 2005. Rapid detection of diarrheagenic E. coli by real-time PCR. J. Microbiol. Methods 61:335-341.
    • (2005) J. Microbiol. Methods , vol.61 , pp. 335-341
    • Bischoff, C.1    Luthy, J.2    Altwegg, M.3    Baggi, F.4
  • 4
    • 0038404937 scopus 로고    scopus 로고
    • Prevalence of Escherichia coli O157:H7 and performance by beef feedlot cattle given Lactobacillus direct-fed microbials
    • Brashears, M. M., M. L. Galyean, G. H. Loneragan, J. E. Mann, and K. Killinger-Mann. 2003. Prevalence of Escherichia coli O157:H7 and performance by beef feedlot cattle given Lactobacillus direct-fed microbials. J. Food Prot. 66:748-754.
    • (2003) J. Food Prot. , vol.66 , pp. 748-754
    • Brashears, M.M.1    Galyean, M.L.2    Loneragan, G.H.3    Mann, J.E.4    Killinger-Mann, K.5
  • 5
    • 84865791547 scopus 로고    scopus 로고
    • Efficacy of a vaccine and a directfed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle
    • Cull, C. A., Z. D. Paddock, T. G. Nagaraja, N. M. Bello, A. H. Babcock, and D. G. Renter. 2012. Efficacy of a vaccine and a directfed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle. Vaccine 30:6210-6215.
    • (2012) Vaccine , vol.30 , pp. 6210-6215
    • Cull, C.A.1    Paddock, Z.D.2    Nagaraja, T.G.3    Bello, N.M.4    Babcock, A.H.5    Renter, D.G.6
  • 6
    • 8344223786 scopus 로고    scopus 로고
    • Screening and diagnostic tests
    • 2nd ed. AVC Inc., Charlottetown, Prince Edward Island
    • Dohoo, I., W. Martin, and H. Stryhn. 2009. Screening and diagnostic tests, p. 91-134. In Veterinary epidemiologic research, 2nd ed. AVC Inc., Charlottetown, Prince Edward Island.
    • (2009) Veterinary Epidemiologic Research , pp. 91-134
    • Dohoo, I.1    Martin, W.2    Stryhn, H.3
  • 7
    • 1642505608 scopus 로고    scopus 로고
    • Application of real-time CPR and RT-PCR assays for the detection and quantification of VT1 and VT2 toxin genes in E. Coli O157:H7
    • Fitzmaurice, J., M. Glennon, G. Duffy, J. J. Sheridan, C. Carroll, and M. Maher. 2004. Application of real-time CPR and RT-PCR assays for the detection and quantification of VT1 and VT2 toxin genes in E. coli O157:H7. Mol. Cell. Probes 18:123-132.
    • (2004) Mol. Cell. Probes , vol.18 , pp. 123-132
    • Fitzmaurice, J.1    Glennon, M.2    Duffy, G.3    Sheridan, J.J.4    Carroll, C.5    Maher, M.6
  • 8
    • 51949105701 scopus 로고    scopus 로고
    • Association between the presence and magnitude of Escherichia coli O157 in feces at harvest and contamination of preintervention beef carcasses
    • Fox, J. T., D. G. Renter, M. W. Sanderson, A. L. Nutsch, X. Shi, and T. G. Nagaraja. 2008. Association between the presence and magnitude of Escherichia coli O157 in feces at harvest and contamination of preintervention beef carcasses. J. Food Prot. 71:1761-1767.
    • (2008) J. Food Prot. , vol.71 , pp. 1761-1767
    • Fox, J.T.1    Renter, D.G.2    Sanderson, M.W.3    Nutsch, A.L.4    Shi, X.5    Nagaraja, T.G.6
  • 10
    • 0037219048 scopus 로고    scopus 로고
    • Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR
    • Ibekwe, A. M., and V. M. Grieve. 2003. Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR. J. Appl. Microbiol. 94:421-431.
    • (2003) J. Appl. Microbiol. , vol.94 , pp. 421-431
    • Ibekwe, A.M.1    Grieve, V.M.2
  • 11
    • 77954647665 scopus 로고    scopus 로고
    • Animal- and truck-load level associations between Escherichia coli O157:H7 in feces and on hides at harvest and contamination of preevisceration beef carcasses
    • Jacob, M. E., D. G. Renter, and T. G. Nagaraja. 2010. Animal- and truck-load level associations between Escherichia coli O157:H7 in feces and on hides at harvest and contamination of preevisceration beef carcasses. J. Food Prot. 73:1030-1037.
    • (2010) J. Food Prot. , vol.73 , pp. 1030-1037
    • Jacob, M.E.1    Renter, D.G.2    Nagaraja, T.G.3
  • 12
    • 84855445037 scopus 로고    scopus 로고
    • Evaluation of a multiplex real-time polymerase chain reaction for the quantification of Escherichia coli O157 in cattle feces
    • Jacob, M. E., X. Shi, B. An, T. G. Nagaraja, and J. Bai. 2012. Evaluation of a multiplex real-time polymerase chain reaction for the quantification of Escherichia coli O157 in cattle feces. Foodborne Pathog. Dis. 9:79-85.
    • (2012) Foodborne Pathog. Dis. , vol.9 , pp. 79-85
    • Jacob, M.E.1    Shi, X.2    An, B.3    Nagaraja, T.G.4    Bai, J.5
  • 13
    • 0017360990 scopus 로고
    • The measurement of observer agreement for categorical data
    • Landis, J. R., and G. G. Koch. 1977. The measurement of observer agreement for categorical data. Biometrics 33:159-174.
    • (1977) Biometrics , vol.33 , pp. 159-174
    • Landis, J.R.1    Koch, G.G.2
  • 14
    • 33644886646 scopus 로고    scopus 로고
    • Sensitivity of Escherichia coli O157 detection in bovine feces assessed by both enrichment followed by immunomagnetic separation and direct plating methodologies
    • Le Jeune, J. T., D. D. Hancock, and T. E. Besser. 2006. Sensitivity of Escherichia coli O157 detection in bovine feces assessed by both enrichment followed by immunomagnetic separation and direct plating methodologies. J. Clin. Microbiol. 44:872-875.
    • (2006) J. Clin. Microbiol. , vol.44 , pp. 872-875
    • Le Jeune, J.T.1    Hancock, D.D.2    Besser, T.E.3
  • 17
    • 0037371268 scopus 로고    scopus 로고
    • Lymphoid follicle-dense mucosa at the terminal rectum is the principle site of colonization of enterohemorrhagic Escherichia coli O157:H7 in the bovine host
    • Naylor, S. W., J. C. Low, T. E. Besser, A. Mahajan, G. J. Gunn, M. C. Pearce, I. J. McKendrick, D. G. E. Smith, and D. L. Gally. 2003. Lymphoid follicle-dense mucosa at the terminal rectum is the principle site of colonization of enterohemorrhagic Escherichia coli O157:H7 in the bovine host. Infect. Immunol. 71:1505-1512.
    • (2003) Infect. Immunol. , vol.71 , pp. 1505-1512
    • Naylor, S.W.1    Low, J.C.2    Besser, T.E.3    Mahajan, A.4    Gunn, G.J.5    Pearce, M.C.6    McKendrick, I.J.7    Smith, D.G.E.8    Gally, D.L.9
  • 18
    • 0037545844 scopus 로고    scopus 로고
    • Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter
    • Omisakin, F., M. MacRae, I. D. Ogden, and N. J. C. Strachan. 2003. Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Appl. Environ. Microbiol. 69:2444-2447.
    • (2003) Appl. Environ. Microbiol. , vol.69 , pp. 2444-2447
    • Omisakin, F.1    MacRae, M.2    Ogden, I.D.3    Strachan, N.J.C.4
  • 19
    • 4444250957 scopus 로고    scopus 로고
    • Development and application of a spiral plating methods for the enumeration of Escherichia coli O157 in bovine faeces
    • Robinson, S. E., E. J. Wright, N. J. Williams, C. A. Hart, and N. P. French. 2004. Development and application of a spiral plating methods for the enumeration of Escherichia coli O157 in bovine faeces. J. Appl. Microbiol. 97:581-589.
    • (2004) J. Appl. Microbiol. , vol.97 , pp. 581-589
    • Robinson, S.E.1    Wright, E.J.2    Williams, N.J.3    Hart, C.A.4    French, N.P.5
  • 20
    • 34547106287 scopus 로고    scopus 로고
    • Sensitivity of direct plating for detection of high levels of E. Coli O157:H7 in bovine fecal samples
    • Sanderson, M. W., S. Sreerama, and T. G. Nagaraja. 2007. Sensitivity of direct plating for detection of high levels of E. coli O157:H7 in bovine fecal samples. Curr. Microbiol. 55:158-161.
    • (2007) Curr. Microbiol. , vol.55 , pp. 158-161
    • Sanderson, M.W.1    Sreerama, S.2    Nagaraja, T.G.3
  • 21
    • 0037472216 scopus 로고    scopus 로고
    • Detectio of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins
    • Sharma, V. K., and E. A. Dean-Nystrom. 2003. Detectio of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Vet. Microbiol. 93:247-260.
    • (2003) Vet. Microbiol. , vol.93 , pp. 247-260
    • Sharma, V.K.1    Dean-Nystrom, E.A.2
  • 22
    • 34248996539 scopus 로고    scopus 로고
    • Development and validation of a most-probablenumber immunomagnetic separation methodology of enumerating Escherichia coli O157 in cattle feces
    • Stephens, T. P., G. H. Loneragan, W. E. Chaney, L. A. Branham, and M. M. Brashears. 2007. Development and validation of a most-probablenumber immunomagnetic separation methodology of enumerating Escherichia coli O157 in cattle feces. J. Food Prot. 70:1072-1075.
    • (2007) J. Food Prot. , vol.70 , pp. 1072-1075
    • Stephens, T.P.1    Loneragan, G.H.2    Chaney, W.E.3    Branham, L.A.4    Brashears, M.M.5
  • 24
    • 77649278032 scopus 로고    scopus 로고
    • Prevalence of Escherichia coli O157:H7 in gut contents of beef cattle at slaughter
    • Walker, C., X. Shi, M. Sanderson, J. Sargeant, and T. G. Nagaraja. 2010. Prevalence of Escherichia coli O157:H7 in gut contents of beef cattle at slaughter. Foodborne Pathog. Dis. 7:249-255.
    • (2010) Foodborne Pathog. Dis. , vol.7 , pp. 249-255
    • Walker, C.1    Shi, X.2    Sanderson, M.3    Sargeant, J.4    Nagaraja, T.G.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.