Development and validation of a most-probable-number-immunomagnetic separation methodology of enumerating Escherichia coli O157 in cattle feces

Journal of Food Protection

Volumn 70, Issue 5, 2007, Pages 1072-1075

  Stephens, T P ^a   Loneragan, G H ^b   Chaney, W E ^a   Branham, L A ^a   Brashears, M M ^a  

^a TEXAS TECH UNIVERSITY   (United States)

^b WEST TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOS; BOVINAE; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 34248996539     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-70.5.1072     Document Type: Article

References (24)

1. Alali, W. Q., J. M. Sargeant, T. G. Nagaraja, and B. M. DeBey. 2004. Effect of antibiotics in milk replacer on fecal shedding of Escherichia coli O157:H7 in calves. J. Anim. Sci. 82:2148-2152.
2. Brashears, M. M., D. Jaroni, and J. Trimble. 2003. Isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157:H7 in cattle. J. Food Prot. 66:355-363.
3. Chapman, P. A., A. T. Cerdan-Malo, M. Ellin, R. Ashton, and M. A. Harkin. 2001. Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK. Int. J. Food Microbiol. 64:139-150.
4. Cochran, W. G. 1950. Estimation of bacterial densities by means of most probable numbers. Biometrics 6:105-116.
5. Curiale, M. 2004. MPN calculator. Available at: http://www. i2workout.com/mcuriale/mpn/index.html. Accessed 27 September 2006.
6. Elder, R. O., J. E. Keen, G. R. Siragusa, G. A. Barkocy-Gallagher, M. Koohmaraie, and W. W. Laegreid. 2000. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proc. Natl. Acad. Sci. USA 97:2999-3003.
7. Fegan, N., G. Higgs, P. Vanderlinde, and P. Desmarchelier. 2004. Enumeration of Escherichia coli O157 in cattle faeces using most probable number (MPN) technique and automated immunomagnetic separation (AIMS). Lett. Appl. Microbiol. 38:56-59.
8. Fox, J. T., D. G. Renter, M. W. Sanderson, and T. G. Nagaraja. 2006. Development of a cost-effective method to enumerate Escherichia coli O157 in cattle feces. J. Anim. Sci. 84(Suppl. 1/J):250.
9. Hancock, D. D., T. E. Besser, D. H. Rice, E. D. Ebel, D. E. Herriott, and L. V. Carpenter. 1998. Multiple sources of Escherichia coli O157 in feedlots and dairy farms in the northwestern USA. Prev. Vet. Med. 35:11-19.
10. Harmon, B. G., C. A. Brown, S. Tkalcic, P. O. E. Mueller, A. Parks, A. V. Jain, T. Zhao, and M. P. Doyle. 1999. Fecal shedding and rumen growth of Escherichia coli O157 in fasted calves. J. Food Prot. 62:574-579.
11. Karmali, M. A., B. T. Steele, M. Petric, and C. Lim. 1983. Sporadic cases of hemolytic uremic syndrome associated with fecal cytotoxin and cytotoxin-producing Escherichia coli. Lancet 19:619-620.
12. Lahti, E., O. Ruoho, L. Rantala, M. L. Hanninen, and T. Honkanen-Buzalski. 2003. Longitudinal study of Escherichia coli O157 in a cattle finishing unit. Appl. Environ. Microbiol. 69:554-561.
13. LeJeune, J. T., T. E. Besser, D. H. Rice, J. L. Berg, R. P. Stilborn, and D. D. Hancock. 2004. Longitudinal study of fecal shedding of Escherichia coli O157:H7 in feedlot cattle: predominance and persistence of specific clonal types despite massive cattle population turnover. Appl. Environ. Microbiol. 70:377-384.
14. LeJeune, J. T., D. D. Hancock, and T. E. Besser. 2006. Sensitivity of Escherichia coli O157 detection in bovine feces assessed by broth enrichment followed by immunomagnetic separation and direct plating methodologies. J. Clin. Microbiol. 44:872-875.
15. Locking, M. E., S. J. O'Brien, W. J. Reilly, E. M. Wright, D. M. Campbell, J. E. Coia, L. M Browning, and C. N. Ramsay. 2001. Risk factors for sporadic cases of Escherichia coli O157 infection: the importance of contact with animal excreta. Epidemiol. Infect. 127:215-220.
16. O'Brien, S. J., G. K. Adak, and C. Gilham. 2001. Contact with farming environment as a major risk factor for Shiga toxin (verocytotoxin)-producing Escherichia coli O157 infection in humans. Emerg. Infect. Dis. 7:1049-1051.
17. Omisakin, F., M. MacRae, I. D. Ogden, and N. J. C. Strachan. 2003. Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Appl. Environ. Microbiol. 69:2444-2447.
18. Pearce, M. C., D. Fenlon, J. C. Low, A. W. Smith, H. I. Knight, J. Evans, G. Foster, B. A. Synge, and G. J. Gunn. 2004. Distribution of Escherichia coli O157 in bovine fecal pats and its impact on estimates of the prevalence of fecal shedding. Appl. Environ. Microbiol. 70:5737-5743.
19. Riley, L. W., R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells, B. R. Davis, R. J. Hebert, E. S. Olcott, L. M. Johnson, N. T. Hargrett, P. A. Blake, and M. L. Cohen. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308:681-685.
20. Robinson, S. E., E. J. Wright, N. J. Williams, C. A. Hart, and N. P. French. 2004. Development and application of a spiral plating method for the enumeration of Escherichia coli O157 in bovine faeces. J. Appl. Microbiol. 97:581-589.
21. U.S. Food and Drug Administration. 2001. Bad bug book. Available at: http://www.cfsan.fda.gov/~mow/chap15.html. Accessed 29 April 2006.
22. Wells, J. G., L. D. Shipman, K. D. Greene, E. G. Sowers, J. H. Green, D. N. Cameron, F. P. Downes, M. L. Martin, P. M. Griffin, S. M. Ostroff, M. E. Potter, R. V. Tauxe, and I. K. Wachsmuth. 1991. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle. J. Clin. Microbiol. 29:985-989.
23. Younts-Dahl, S. M., M. L. Galyean, G. H. Loneragan, N. A. Elam, and M. M. Brashears. 2004. Dietary supplementation with lactobacillus- and propionibacterium-based direct-fed microbials and prevalence of Escherichia coli O157 in beef feedlot cattle and on hides at harvest. J. Food Prot. 67:889-893.
24. Younts-Dahl, S. M., G. D. Osborn, M. L. Galyean, J. D. Rivera, G. H. Loneragan, M. M. Brashears. 2005. Reduction of Escherichia coli O157 in finishing beef cattle by various doses of Lactobacillus ac-idophilus in direct-fed microbials. J. Food Prot. 68:6-10.