Evaluation of a multiplex real-time PCR method for detecting Shiga toxin-producing Escherichia coli in beef and comparison to the U.S. Department of Agriculture food Safety and Inspection Service Microbiology Laboratory Guidebook method

Journal of Food Protection

Volumn 77, Issue 2, 2014, Pages 180-188

  Fratamico, Pina M ^a   Wasilenko, Jamie L ^a,b,d   Garman, Bradley ^a   Demarco, Daniel R ^c   Varkey, Stephen ^c   Jensen, Mark ^c   Rhoden, Kyle ^c   Tice, George ^c  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

^b RUSSELL RESEARCH CENTER   (United States)

^c DUPONT COMPANY   (United States)

^d U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI PROTEIN;

ANALYSIS; ANIMAL; BOVINE; FOOD CONTAMINATION; FOOD CONTROL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MEAT; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STANDARDS; UNITED STATES;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CATTLE; ESCHERICHIA COLI PROTEINS; FOOD CONTAMINATION; FOOD INSPECTION; FOOD MICROBIOLOGY; HUMANS; MEAT; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI; UNITED STATES;

EID: 84893439624     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X.JFP-13-248     Document Type: Article

References (23)

1. Anklam, K. S., T. Kanankege, T. K. Gonzalez, C. W. Kaspar, and D. Döpfer. 2012. Rapid and reliable detection of Shiga toxin-producing Escherichia coli by real-time multiplex PCR. J. Food Prot. 75:643-650.
2. Barlow, R. S., and B. E. Mellor. 2010. Prevalence of enterohemorrhagic Escherichia coli serotypes in Australian beef cattle. Foodborne Pathog. Dis. 7:1239-1245.
3. Boerlin, P., S. A. McEwen, F. Boerlin-Petzold, J. B. Wilson, R. P. Johnson, and C. L. Gyles. 1999. Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J. Clin. Microbiol. 37:497-503.
4. Bosilevac, J. M., and M. Koohmaraie. 2011. Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli isolates from commercial ground beef in the United States. Appl. Environ. Microbiol. 77:2103-2112.
5. Botkin, D. J., L. Galli, V. Sankarapani, M. Soler, M. Rivas, and A. G. Torres. 2012. Development of a multiplex PCR assay for detection of Shiga toxin-producing Escherichia coli, enterohemorrhagic E. coli, and enteropathogenic E. coli strains. Front. Cell. Infect. Microbiol. 2:8. doi:10.3389/fcimb.2012.00008.
6. Brooks, J. T., E. G. Sowers, J. G. Wells, K. D. Greene, P. M. Griffin, R. M. Hoekstra, and N. A. Strockbine. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192:1422-1429.
7. Fratamico, P. M., L. K. Bagi, W. C. Cray, Jr., N. Narang, X. Yan, M. Medina, and Y. Liu. 2011. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis. 8:601-607.
8. Hussein, H. S. 2007. Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their products. J. Anim. Sci. 85:E63-E72.
9. Jinneman, K., K. J. Yoshitomi, and S. D. Weagant. 2003. Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H2 serotype. Appl. Environ. Microbiol. 69:6327-6333.
10. Kagkli, D.-M., S. Folloni, E. Barbau-Piednoir, G. Van Den Eede, and M. Van Den Bulcke. 2012. Towards a pathogenic Escherichia coli detection platform using multiplex SYBRH Green real-time PCR methods and high resolution melting analysis. PloS One 7(6):e39287. doi:10.1371/journal.pone.0039287.
11. Mathusa, E. C., Y. Chen, E. Enache, and L. Hontz. 2010. Non-O157 Shiga toxin-producing Escherichia coli in foods. J. Food Prot. 73:1721-1736.
12. Medina, M. B., W. L. Shelver, P. M. Fratamico, L. Fortis, G. Tillman, N. Narang, W. C. Cray, Jr., E. Esteban, and C. Deb Roy. 2012. Latex agglutination assays for detection of non-O157 Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145. J. Food Prot. 75:819-826.
13. Paddock, Z., X. Shi, J. Bai, and T. G. Nagaraja. 2012. Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces. Vet. Microbiol. 156:381-388.
14. Ritchie, J. M., C. M. Thorpe, A. B. Rogers, and M. K. Waldor. 2003. Critical roles for stx2, eae, and tir in enterohemorrhagic Escherichia coli-induced diarrhea and intestinal inflammation in infant rabbits. Infect. Immun. 71:7129-7139.
15. Scallan, E., R. M. Hoekstra, F. J. Angulo, R. V. Tauxe, M.-A. Widdowson, S. L. Roy, J. L. Jones, and P. M. Griffin. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17:7-14.
16. Tillman, G. E., J. L. Wasilenko, M. Simmons, T. A. Lauze, J. Minicozzi, B. B. Oakley, N. Narang, P. Fratamico, and W. C. Cray, Jr. 2012. Isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O121, and O145 from ground beef using modified Rainbow agar and post-immunomagnetic separation acid treatment. J. Food Prot. 75:1548-1554.
17. U. S. Department of Agriculture, Food Safety and Inspection Service. 2010. FSIS procedure for use of Escherichia coli O157:H7 screening tests chap. 5A.02. In Microbiology laboratory guidebook. Available at: http://www.fsis.usda. gov/PDF/Mlg-5A-02.pdf. Accessed 28 May 2013.
18. U. S. Department of Agriculture, Food Safety and Inspection Service. 2011. Shiga toxin-producing Escherichia coli in certain raw beef products. Fed. Regist. 76(182):58157-58165.
19. U. S. Department of Agriculture, Food Safety and Inspection Service. 2012. Detection, isolation and identification of Escherichia coli O157:H7 from meat products, chap. 5.06. In Microbiology laboratory guidebook. Available at: http://www.fsis.usda.gov/PDF/MLG-5-06.pdf. Accessed 28 May 2013.
20. U. S. Department of Agriculture, Food Safety and Inspection Service. 2012. Detection and isolation of non-O157 Shiga toxin-producing Escherichia coli (STEC) from meat products, chap. 5B.03. In Microbiology laboratory guidebook. Available at: http://www.fsis. usda.gov/PDF/MLG-5B.pdf. Accessed 28 May 2013.
21. Vimont, A., M. L. Delignette-Muller, and C. Vernozy-Rozand. 2007. Supplementation of enrichment broths by novobiocin for detecting Shiga toxin-producing Escherichia coli from food: a controversial use. Lett. Appl. Microbiol. 44:326-331.
22. Wasilenko, J. L., P. M. Fratamico, N. Narang, G. E. Tillman, S. Ladely, M. Simmons, and W. C. Cray, Jr. 2012. Influence of primer sequences and DNA extraction method on detection of non-O157 Shiga toxin-producing Escherichia coli in ground beef by real-time PCR targeting eae, stx, and serogroup-specific genes. J. Food Prot. 75:1939-1950.
23. Werber, D., A. Fruth, U. Buchholz, R. Prager, M. H. Kramer, A. Ammon, and H. Tschäpe. 2003. Strong association between Shiga toxin-producing Escherichia coli O157 virulence genes stx2 and eae as possible explanation for predominance of serogroup O157 in patients with hemolytic uremic syndrome. Eur. Clin. Microbiol. Infect. Dis. 22:726-730.