Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces

Journal of Microbiological Methods

Volumn 105, Issue , 2014, Pages 72-79

  Luedtke, Brandon E ^a   Bono, James L ^a   Bosilevac, Joseph M ^a  

^a U S MEAT ANIMAL RESEARCH CENTER   (United States)

Author keywords

Enterohemorrhagic E. coli; Limit of detection; Limit of quantification; Multiplex qPCR; Non O157 Shiga toxin producing E. coli

Indexed keywords

SHIGA TOXIN; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL SHEDDING; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CARCASS; COLONY FORMING UNIT; ENTEROHEMORRHAGIC ESCHERICHIA COLI; FECES ANALYSIS; FOOD SAFETY; GENE TARGETING; LIMIT OF DETECTION; LIMIT OF QUANTITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY ANALYSIS; SEROTYPE; UNITED STATES; ANIMAL; BACTERIAL LOAD; BOVINE; CATTLE DISEASES; COMPARATIVE STUDY; ESCHERICHIA COLI INFECTIONS; EVALUATION STUDY; FECES; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; PROCEDURES; SENSITIVITY AND SPECIFICITY; VETERINARY;

ANIMALS; BACTERIAL LOAD; CATTLE; CATTLE DISEASES; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FECES; FOOD SAFETY; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UNITED STATES;

EID: 84905216517     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.07.015     Document Type: Article

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