Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods

Journal of Applied Microbiology

Volumn 103, Issue 5, 2007, Pages 1657-1668

  Brichta Harhay, D M ^a   Arthur, T M ^a   Bosilevac, J M ^a   Guerini, M N ^a   Kalchayanand, N ^a   Koohmaraie, M ^a  

^a U S MEAT ANIMAL RESEARCH CENTER   (United States)

Author keywords

Beef; Carcass; Enumeration; Escherichia coli; HGMF; O157; Salmonella; Spiral plate

Indexed keywords

COST EFFECTIVENESS; ESCHERICHIA COLI; MICROFILTRATION; SALMONELLA; THROUGHPUT;

CARCASS; E.COLI O157:H7; ENUMERATION; FAECAL SAMPLES; GROUND BEEF; HYDROPHOBIC GRID MEMBRANE FILTRATION; HYDROPHOBICS; MEMBRANE FILTRATIONS; O157; SPIRAL PLATE;

BEEF;

CATTLE; COLIFORM BACTERIUM; ESTIMATION METHOD; FECAL PELLET; HYDROPHOBICITY;

ARTICLE; BACTERIUM DETECTION; CATTLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DATA COLLECTION METHOD; ESCHERICHIA COLI; FECES ANALYSIS; MEMBRANE FILTER; NONHUMAN; PLATING MEDIUM; SALMONELLA TYPHIMURIUM; VALIDATION STUDY;

BOS; ESCHERICHIA COLI; SALMONELLA;

EID: 35449007735     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03405.x     Document Type: Article

References (37)

1. Arroyo, G. Arroyo, J.A. (1995) Selective action of inhibitors used in different culture media on the competitive microflora of Salmonella. J Appl Bacteriol 78, 281 289.
2. Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Kent, M.P., Jaroni, D., Pauling, B., et al. (2004) Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriaciae, and Escherichia coli O157 at various steps in commercial beef processing plants. J Food Prot 67, 658 665.
3. Barkocy-Gallagher, G.A., Berry, E.D., Rivera-Betancourt, M., Arthur, T.M., Nou, X. Koohmaraie, M. (2002) Development of methods for the recovery of Escherichia coli O157:H7 and Salmonella from beef carcass sponge samples and bovine fecal and hide samples. J Food Prot 65, 1527 1534.
4. Barkocy-Gallagher, G.A., Arthur, T.M., Rivera-Bentacourt, M., Nou, X., Shackelford, S.D., Wheeler, T.L. Koohmaraie, M. (2003) Seasonal prevalence of shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. J Food Prot 66, 1978 1986.
5. Barkocy-Gallagher, G.A., Edwards, K.K., Nou, X., Bosilevac, J.M., Arthur, T.M., Shackelford, S.D. Koohmaraie, M. (2005) Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples: sensitivity and improvements. J Food Prot 68, 2264 2268.
6. Blackburn, C.deW. McCarthy, J.D. (2000) Modifications to methods for the enumeration and detection of injured Escherichia coli O157:H7 in foods. Int J Food Microbiol 55, 285 290.
7. Blodgett, R. Garthright, W.E. (1998) Several MPN models for serial dilutions with suppressed growth at low dilutions. Food Microbiol 15, 91 99.
8. Blodgett, R. (2001) Bacteriological analytical manual on-line, appendix 2, most probable number from serial dilutions. Available at: http://www.cfsan.fda.gov/∼ebam/bam-a2.html.
9. Bosilevac, J.M., Nou, X., Barkocy-Gallagher, G.A., Arthur, T.M. Koohmaraie, M. (2006) Treatments using hot water instead of lactic acid reduce levels of aerobic bacteria and Enterobacteriaceae and reduce the prevalence of Escherichia coli O157:H7 on preevisceration beef carcasses. J Food Prot 69, 1808 1813.
10. Clough, H.E., Clancy, D., O'Neill, P.D., Robinson, S.E. French, N.P. (2005) Quantifying uncertainty associated with microbial count data: a bayesian approach. Biometrics 61, 610 616.
11. Cochran, W.G. (1950) Estimation of bacterial densities by means of the "most probable number". Biometrics 6, 105 116.
12. deMan, J.C. (1983) MPN tables, corrected. Eur J Appl Microbiol Biotech 17, 301 305.
13. Echeverry, A., Loneragan, G.H., Wagner, B.A. Brashears, M.M. (2005) Effect of intensity of fecal pat sampling on estimates of Escherichia coli O157 prevalence. Am J Vet Res 66, 2023 2027.
14. Entis, P., Brodsky, M.H., Sharpe, A.N. Jarvis, G.A. (1982) Rapid detection of Slamonella spp. in food by use of the iso-grid hydrophobic grid membrane filter. Appl Environ Microbiol 43, 261 268.
15. Fegan, N., Higgs, G., Vanderlinde, P. Desmachelier, P. (2003) Enumeration of Escherichia coli O157 in cattle faeces using most probable number technique and automated immunomagnetic separation. Lett Appl Microbiol 38, 56 59.
16. Fegan, N., Vanderlinde, P., Higgs, G. Desmarchelier, P. (2004) Quantification and prevalence of Salmonella in beef cattle presenting at slaughter. J Appl Microbiol 97, 892 898.
17. Fegan, N., Vanderlinde, P., Higgs, G. Desmarchelier, P. (2005) A study of the prevalence and enumeration of Salmonella enterica in cattle and on carcasses during processing. J Food Prot 68, 1147 1153.
18. Gilchrist, J.E., Campbell, J.E., Donnelly, C.B., Peeler, J.T. Delaney, J.M. (1973) Spiral plate method for bacterial determination. Appl Microbiol 25, 244 252.
19. Guy, R.A., Kapoor, A., Holicka, J., Shepherd, D. Horgen, P.A. (2006) A rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses. J Food Prot 69, 1265 1272.
20. Hu, Y., Zhang, Q. Meitzler, J.C. (1999) Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by multiplex PCR. J Appl Microbiol 87, 867 876.
21. McDaniels, A.E., Bordner, R.H., Menkedick, J.R. Weber, C.I. (1987) Comparison of the hydrophobic-grid membrane filter procedure and standard methods for coliform analysis of water. Appl Environ Microbiol 53, 1003 1009.
22. Nou, X., Arthur, T.M., Bosilevac, J.M., Brichta, D.M., Guerini, M.N., Kalchayanand, N. Koohmaraie, M. (2006) Improvement of immunomagnetic separation for Escherichia coli O157:H7 detection by PickPen magnetic particle separation device. J Food Prot 12, 2870 2874.
23. Nucera, D.M., Maddox, C.W., Hoien-Dalen, P. Weigel, R.M. (2006) Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units. J Clin Microbiol 44, 3388 3390.
24. Nystrom, T. (2001) Not quite dead enough: on bacterial life, culturability, senescence, and death (review). Arch Microbiol 176, 159 164.
25. Ogden, I.D., MacRae, M., Hepburn, N.F. Strachan, N.J.C. (2000) Improved isolation of Escherichia coli O157 using large enrichment volumes for immunomagnetic separation. Lett Appl Microbiol 31, 338 341.
26. Omisakin, F., MacRae, M., Ogden, I.D. Strachan, J.C. (2003) Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Appl Environ Micro 69, 2444 2447.
27. Pearce, M.C., Fenlon, D., Low, J.C., Smith, A.W., Knight, H.I., Evans, J., Foster, G., Synge, B.A., et al. (2004) Distribution of Escherichia coli O157 in bovine fecal pats and its impact on estimates of the prevalence of fecal shedding. Appl Environ Micro 70, 5737 5743.
28. Rahn, K., DeGrandis, S.A., Clarke, R.C., McEwen, S.A., Galan, J.E., Ginocchio, C., Curtiss, R. Gyles, C.L. (1992) Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 6, 271 279.
29. Robinson, S.E., Wright, E.J., Williams, N.J., Hart, C.A. French, N.P. (2004a) Development and application of a spiral plating method for the enumeration of Escherichia coli O157 in bovine feces. J Appl Microbiol 97, 581 589.
30. Robinson, S.E., Wright, E.J., Hart, C.A., Bennet, M. French, N.P. (2004b) Intermittent and persistent shedding of Escherichia coli O157 in cohorts of naturally infected calves. J Appl Microbiol 97, 1045 1053.
31. Robinson, S.E., Brown, P.E., Wright, E.J., Bennett, M., Hart, C.A. French, N.P. (2005) Heterogeneous distributions of Escherichia coli O157:H7 within naturally infected bovine fecal pats. FEMS Microbiol Lett 244, 291 296.
32. Sharpe, A.N. Michaud, G.L. (1974) Hydrophobic grid-membrane filters: new approach to microbiological enumeration. Appl Microbiol 28, 223 225.
33. Sharpe, A.N., Hearn, E.M. Kovacs-Nolan, J. (2000) Comparison of membrane filtration rates and hydrophobic grid membrane filter coliform and Escherichia coli counts in food suspensions using paddle-type and pulsifier sample preparation procedures. J Food Prot 63, 126 130.
34. Synge, B.A. Gunn, G.J. (2004) Distribution of Escherichia coli O157 in bovine fecal pats and its impact on estimates of the prevalence of fecal shedding. Appl Environ Microbiol 70, 5737 5743.
35. Szabo, R., Todd, E., MacKenzie, J., Parrington, L. Armstrong, A. (1990) Increased sensitivity of the rapid hydrophobic grid membrane filter enzyme-labeled antibody procedure for Escherichia coli O157:H7 detection in foods and bovine feces. Appl Environ Microbiol 56, 3546 3549.
36. Wohlsen, T., Bates, J., Vesey, G., Robinson, W.A. Katouli, M. (2006) Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards. Lett Appl Microbiol 42, 350 356.
37. Wolffs, P.F.G., Glencross, K., Thibaudeau, R. Griffiths, M.W. (2006) Direct quantitation and detection of Salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. App Environ Microbiol 72, 3896 3900.