Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR

Journal of Applied Microbiology

Volumn 94, Issue 3, 2003, Pages 421-431

  Ibekwe, A M ^a   Grieve, C M ^a  

^a U S SALINITY LABORATORY   (United States)

Author keywords

Escherichia coli O157:H7; Faeces; Fluorescence threshold; Manure; Multiplex PCR; Real time PCR

Indexed keywords

ESCHERICHIA COLI; FERTILIZERS; MANURES; POLYMERASE CHAIN REACTION; RISK ASSESSMENT; SOILS;

COLONY COUNTS; DETECTION AND QUANTIFICATIONS; E.COLI O157:H7; ENVIRONMENTAL SAMPLE; ESCHERICHIA COLI O157:H7; FECES; FLUORESCENCE THRESHOLD; MULTIPLEX POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTIONS; WASHWATER;

FLUORESCENCE;

BACTERIUM; DETECTION METHOD; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL COUNT; BACTERIUM DETECTION; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI; MANURE; MICROBIAL CONTAMINATION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RISK ASSESSMENT; SAMPLING; SOIL POLLUTION; WASTE WATER MANAGEMENT; WATER CONTAMINATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037219048     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.01848.x     Document Type: Article

References (36)

1. Ackers, M.L., Mahon, B.E., Leahy, E., Goode, B., Damrow, T., Hayes, P.S., Bibb, W.F., Rice D.H., Barrett T.J., Hutwagner L., Griffin P.M., and Slutsker, L. (1998) An outbreak of Escherichia coli O157:H7 infection associated with leaf lettuce consumption. Journal of Infectious Diseases 177, 1588-1593
2. Agin, T.S., Cantey, J.R., Boedeker, E.C. and Wolf, M.K. (1996) Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-effacing lesions. FEMS Microbiology Letter 144, 249-258.
3. Altekruse, S.F., Cohen, M.L. and Swerdlow, D.L. (1997) Emerging foodborne diseases. Emerging Infectious Diseases 3, 285-293.
4. Bassler, H.A., Flood, S.J., Livak, K.J., Marmaro, J., Knorr, R., and Batt, C.A. (1995) Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Applied and Environmental Microbiology 61, 3724-3728.
5. Belgrader, P., Okuzumi, M., Pourahmadi, F., Borkholder, D.A. and Northrup, M.A. (2000) A microfluidic cartridge to prepare spores for PCR analysis. Biosensors and Bioelectronics 14, 849-852.
6. Bellin, T., Pulz, M., Matusset, A., Hempen, H.-G. and Gunzer, F. (2001). Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes. Journal of Clinical Microbiology 39, 370-374
7. Biosearch Technologies. (2000) Black Hole Quencher Dyes. The Inescapable Solution for DNA Hybridization Probes. Novato, CA.
8. Campbell, G.R., Prosser, J., Glover, A. and Killham, K. (2001) Detection of Escherichia coli O157:H7 in soil and water using multiplex PCR. Journal of Applied Microbiology 91, 1004-1010.
9. Chapman, P.A., Siddons, C.A., Cerdan Malo, A.T., and Harkin, M.A. (1997) A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiology and Infection 119, 245-250.
10. Dorn, C.R. and Angrick, E.J. (1991) Serotype O157:H7 Escherichia coli from bovine and meat sources. Journal of Clinical Microbiology 29, 1225-1251.
11. Elder, R.O., Keen, J.E., Siragusa, G.R., Barkocy-Gallagher, G.A., Koohmaraie, M. and Laaegreid, W.W. (2000) Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proceedings of the National Academy of Sciences of the United States of America 97, 2999-3003.
12. Franke, S., Harmsen, D. Caprioli, A. Pierard, D. Wieler, L.H. and Karch, H. (1995) Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 of human and porcine origin. Journal of Clinical Microbiology 33, 3174-3178.
13. Fratamico, P.M., Bagi, L.K. and Pepe, T. (2000) A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. Journal of Food Protection 63, 1032-1037.
14. Gagliardi, J.V. and Karns, J.S. (2000) Leaching of Escherichia coli O157:H7 in diverse soils under various agricultural management practices. Applied and Environmental Microbiology 66, 877-883.
15. Heid, C.A., Stevens, J., Livak, K.J. and Williams P.M. (1996) Real time quantitative PCR. Genome Research 6, 986-994.
16. Hillborn, E.D., Mermin, J.H. Mshar, P.A. Hadler, J.L. Voetsch, A. Wojtkunski, C. Swartz, M. Mshar, R. Lambert-Fair, M.A. Farrar, J.A. Glynn, M.K. and Slutsker, L. (1999) A multistate outbreak of Escherichia coli O157:H7 infections associated with consumption of mesclun lettuce. Archives of Internal Medicine. 159, 1758-1764
17. Hristova, K.R., Lutenegger, C.M. and Scow K.M. (2001) Detection and quantification of methyl tert-butyl ether-degrading strain PMI by real-time TaqMan PCR. Applied and Environmental Microbiology 67, 5154-5160.
18. Hu, Y., Zhang, Q. and Meitzler, J.C. (1999) Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR. Journal of Applied Microbiology 87, 867-876.
19. Karmali, M.A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clinical Microbiology Review. 2, 15-38.
20. Lee, L.G., Connell, C.R. and Bloch, W. (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Research 21, 3761-3766.
21. Louie, M., De Azavedo, J.C.S., Handelsman, M.Y.C., Clark, C.G., Ally, B., Dytoe, M., Sherman, P. and Brunton, J. (1993) Expression and characterization of the eaeA gene product of Escherichia coli serotype O157:H7. Infection and Immunity 61, 4085-4092.
22. Luna, G.M., Manini, E. and Danovaro, R. (2002) Large fragment of dead and inactivated bacteria in coastal marine sediments: comparison of protocols for determination and ecological significance. Applied and Environmental Microbiology 68, 3509-3513.
23. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S. and Kaper, J. B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proceedings of the National Academy of Sciences of the United States of America 92, 1664-1668.
24. McKee, M.L., Melton-Celsa, A.R., Moxley, R.A., Francis, D.H. and O'Brien, A.D. (1995) Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infection and Immunity 63, 3739-3744.
25. Oberst, R.D., Hays, M.P., Bohra, L.K., Phebus, R.K., Yamashiro, C.T., Paszko-Kolva, C., Flood, S.J.A., Sargeant, J.M. and Gillespie, J.R. (1998) PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5' nuclease assay. Applied and Environmental Microbiology 64, 3389-3396.
26. O'Brien, A.D. and Holmes, R.K. (1987) Shiga and the Shiga-like toxins. Microbiology Review 51, 206-220.
27. Obrig, T.G. (1992) Pathogenesis of Shiga toxin (verotoxin)-induced endothelial cell injury. In Hemolytic Uremic Syndrome and Thrmobocylpenic Purpura ed. Kaplan, B.S., Trompeter, R.S., and Moakc, J.L. pp. 405-419. New York: Marcel Dekker, Inc.
28. O'Conner, D.R. (2002) Report of the Walkerton Inquiry. The events of May, 2000 and related issues (WWW.Walkertoninquiry.com). Queen's printer for Ontorio.
29. Sharma, V.K. and Carlson, S.A. (2000) Simultaneous detection of Salmonella strains and Escherichia coli O157:H7 with fluorogenic PCR and single enrichment-broth culture. Applied and Environmental. Microbiology 66, 5472-5476.
30. Sharma, V.K., Dean-Nystrom, E.A. and Casey, T.A. (1999) Semi-automated fluorogenic PCR assays (TaqMan) for rapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli. Molecular and Cellular Probes 13, 291-302.
31. Shere, J.A., Bartlett, K.J. and Kaspar, C.W. (1998) Longitudinal study of Escherichia coli O157:H7 dissemination on four dairy farms in Wisconsin. Applied and Environmental Microbiology 64, 1390-1399.
32. Slutsker, L., Altekruse, S.F. and Swerdlow, D.L. (1998) Foodborne diseases emerging pathogens and trends. Infectious Disease Clinics of North America 12, 199-216.
33. Taylor, T.B., Winn-Deen, E.S., Picozza, E., Woudenberg, T.M. and Albin, M. (1997) Optimization of the performance of the polymerase chain reaction in silicon-based microstructures. Nucleic Acids Research 25, 3164-3168.
34. Tebbe, C.C. and Vahjen, W. (1993) Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast. Applied and Environmental Microbiology 59, 2657-2665.
35. U.S. EPA. (1996) National Water Quality Inventory, Report to Congress, EPA 841-R-97-008; Washington, DC: U.S. EPA.
36. Zhao, T., Doyle, M.P., Shere, J. and Garber, L. (1995) Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds. Applied and Environmental Microbiology 61, 1290-1293.