Quantification of E. coli O157 and STEC in feces of farm animals using direct multiplex real time PCR (qPCR) and a modified most probable number assay comprised of immunomagnetic bead separation and qPCR detection

Journal of Microbiological Methods

Volumn 99, Issue 1, 2014, Pages 44-53

  Guy, Rebecca A ^a,b   Tremblay, Donald ^b   Beausoleil, Louise ^a   Harel, Josée ^b   Champagne, Marie Josée ^a  

^a PUBLIC HEALTH AGENCY OF CANADA   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

E. coli O157 STEC; Modified MPN; On farm; Quantification; Real time PCR; Shiga toxin

Indexed keywords

VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL CONCENTRATION; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL TRANSMISSION; BACTERIUM DETECTION; BEEF CATTLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DAIRY CATTLE; EAEA GENE; ESCHERICHIA COLI O157; FARM ANIMAL; FECES ANALYSIS; IMMUNOMAGNETIC BEAD SEPARATION; IMMUNOMAGNETIC SEPARATION; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MODIFIED MOST PROBABLE NUMBER METHOD; MOST PROBABLE NUMBER METHOD; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PER GENE; POULTRY; PRIORITY JOURNAL; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STX1 GENE; STX2 GENE; SWINE;

E. COLI O157/STEC; MODIFIED MPN; ON-FARM; QUANTIFICATION; REAL-TIME PCR; SHIGA-TOXIN;

ANIMALS; ANIMALS, DOMESTIC; BACTERIAL LOAD; FECES; IMMUNOMAGNETIC SEPARATION; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84896817237     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.02.002     Document Type: Article

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