Single-molecule imaging of Hedgehog pathway protein Smoothened in primary cilia reveals binding events regulated by Patched1

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 27, 2015, Pages 8320-8325

  Milenkovic, Ljiljana ^a   Weiss, Lucien E ^a   Yoon, Joshua ^a   Roth, Theodore L ^a   Su, You Rong S ^a   Sahl, Steffen J ^a   Scott, Matthew P ^b   Moerner, W E ^a  

^a STANFORD UNIVERSITY   (United States)

^b GEOPHYSICAL LABORATORY   (United States)

Author keywords

Hedgehog signaling; Primary cilia; Single molecule microscopy; Single particle tracking; Smoothened

Indexed keywords

PERICENTRIN; PROTEIN PATCHED 1; SMOOTHENED PROTEIN; SONIC HEDGEHOG PROTEIN; TRANSCRIPTION FACTOR GLI1; CELL SURFACE RECEPTOR; G PROTEIN COUPLED RECEPTOR; PATCHED RECEPTORS; PROTEIN BINDING; SMO PROTEIN, MOUSE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; BLEACHING; CONTROLLED STUDY; DISSOCIATION; DISSOCIATION CONSTANT; EMBRYO; EUKARYOTIC FLAGELLUM; FIBROBLAST; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSCRIPTION; MOLECULAR IMAGING; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION; SINGLE MOLECULE IMAGING; ALGORITHM; ANIMAL; CELL CULTURE; CELL TRACKING; CONFOCAL MICROSCOPY; CYTOLOGY; GENETICS; KINETICS; KNOCKOUT MOUSE; MAMMALIAN EMBRYO; METABOLISM; PROCEDURES; TRANSGENIC MOUSE;

ERINACEIDAE;

ALGORITHMS; ANIMALS; CELL TRACKING; CELLS, CULTURED; CILIA; EMBRYO, MAMMALIAN; FIBROBLASTS; HEDGEHOG PROTEINS; KINETICS; MICE, KNOCKOUT; MICE, TRANSGENIC; MICROSCOPY, CONFOCAL; PROTEIN BINDING; RECEPTORS, CELL SURFACE; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION;

EID: 84936817681     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1510094112     Document Type: Article

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