The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin

EMBO Reports

Volumn 1, Issue 6, 2000, Pages 524-529

  Gillingham, Alison K ^a   Munro, Sean ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AKAP9 PROTEIN, HUMAN; ANTIGEN; CALMODULIN; CARRIER PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYTOSKELETON PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; PERICENTRIN; PHOTOPROTEIN; SIGNAL TRANSDUCING ADAPTOR PROTEIN; TUBULIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL STRAIN COS1; CENTROSOME; CHEMISTRY; DROSOPHILA; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY; ULTRASTRUCTURE;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS; CALMODULIN; CARRIER PROTEINS; CENTROSOME; CONSERVED SEQUENCE; COS CELLS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; CYTOSKELETAL PROTEINS; DROSOPHILA; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFECTION; TUBULIN;

EID: 0034574615     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kvd105     Document Type: Article

References (20)

1. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25, 3389-3402.
2. Dasgupta, M., Honeycutt, T. and Blumenthal, D.K. (1989) The γ-subunit of skeletal muscle phosphorylase kinase contains two noncontiguous domains that act in concert to bind calmodulin. J. Biol. Chem., 264, 17156-17163.
3. Dictenberg, J.B., Zimmerman, W., Sparks, C.A., Young, A., Vidair, C., Zheng, Y., Carrington, W., Fay, F.S. and Doxsey, S.J. (1998) Pericentrin and γ-tubulin form a protein complex and are organized into a novel lattice at the centrosome. J. Cell Biol., 141, 163-174.
4. Diviani, D., Langeberg, L.K., Doxsey, S.J. and Scott, J.D. (2000) Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain. Curr. Biol., 10, 417-420.
5. Doxsey, S.J., Stein, P., Evans, L., Calarco, P.D. and Kirschner, M. (1994) Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell, 76, 639-650.
6. Flory, M.R., Moser, M.J., Monnat, R.J. and Davis, T.N. (2000) Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin. Proc. Natl Acad. Sci. USA, 97, 5919-5923.
7. Geiser, J.R., Sundberg, H.A., Chang, B.H., Muller, E.G. and Davis, T.N. (1993) The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol. Cell. Biol., 13, 7913-7924.
8. Jurado, L.A., Chockalingam, P.S. and Jarrett, H.W. (1999) Apocalmodulin. Physiol. Rev., 79, 661-682.
9. Li, C.J., Heim, R., Lu, P., Pu, Y., Tsien, R.Y. and Chang, D.C. (1999) Dynamic redistribution of calmodulin in HeLa cells during cell division as revealed by a GFP-calmodulin fusion protein technique. J. Cell Sci., 112, 1567-1577.
10. Moser, M.J., Flory, M.R. and Davis, T.N. (1997) Calmodulin localizes to the spindle pole body of Schizosaccharomyces pombe and performs an essential function in chromosome segregation. J. Cell Sci., 110, 1805-1812.
11. Munro, S. and Nichols, B.J. (1999) The GRIP domain - a novel Golgi-targeting domain found in several coiled-coil proteins. Curr. Biol., 9, 377-380.
12. Glued and a novel actin. J. Biol. Chem., 268, 15318-15323.
13. Purohit, A., Tynan, S.H., Vallee, R. and Doxsey, S.J. (1999) Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization. J. Cell Biol., 147, 481-492.
14. Schiebel, E. (2000) y-tubulin complexes: binding to the centrosome, regulation and microtubule nucleation. Curr. Opin. Cell Biol., 12, 113-118.
15. Schmidt, P.H., Dransfield, D.T., Claudio, J.O., Hawley, R.G., Trotter, K.W., Milgram, S.L. and Goldenring, J.R. (1999) AKAP350, a multiply spliced protein kinase A-anchoring protein associated with centrosomes. J. Biol. Chem., 274, 3055-3066.
16. Shevchenko, A. et al. (1996) Linking genome and proteome by mass spectrometry-large-scale identification of yeast proteins from 2-dimensional gels. Proc. Nall Acad. Sci. USA, 93, 14440-14445.
17. Stirling, D.A., Welch, K.A. and Stark, M.J. (1994) Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO J., 13, 4329-4342.
18. Takahashi, M., Shibata, H., Shimakawa, M., Miyamoto, M., Mukai, H. and Ono, Y. (1999) Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the Golgi apparatus. J. Biol. Chem., 274, 17267-17274.
19. Witczak, O., Skalhegg, B.S., Keryer, G., Bornens, M., Tasken, K., Jahnsen, T. and Orstavik, S. (1999) Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450. EMBO J., 18, 1858-1868.
20. Zimmerman, W., Sparks, C.A. and Doxsey, S.J. (1999) Amorphous no longer: the centrosome comes into focus. Curr. Opin. Cell Biol., 11, 122-128.