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Volumn 118, Issue 9, 2005, Pages 1799-1809

Single-molecule diffusion measurements of H-Ras at the plasma membrane of live cells reveal microdomain localization upon activation

Author keywords

Fluorescence microscopy; H Ras; Microdomains; Single molecule diffusion

Indexed keywords

INSULIN; MUTANT PROTEIN; ONCOPROTEIN; YELLOW FLUORESCENT PROTEIN;

EID: 19444382673     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.02300     Document Type: Article
Times cited : (103)

References (49)
  • 1
    • 77957083058 scopus 로고
    • Lateral diffusion in membranes
    • (ed. R. Lipowsky and E. Sackmann), Amsterdam: Elsevier/North Holland
    • Almeida, P. F. F. and Vaz, W. L. C. (1995). Lateral diffusion in membranes. In Handbook of Biological Physics (ed. R. Lipowsky and E. Sackmann), pp. 305-357. Amsterdam: Elsevier/North Holland.
    • (1995) Handbook of Biological Physics , pp. 305-357
    • Almeida, P.F.F.1    Vaz, W.L.C.2
  • 2
    • 0026528045 scopus 로고
    • Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C
    • Anderson, C. M., Georgiou, G. N., Morrison, I. E., Stevenson, G. V. and Cherry, R. J. (1992). Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C. J. Cell Sci. 101, 415-425.
    • (1992) J. Cell Sci. , vol.101 , pp. 415-425
    • Anderson, C.M.1    Georgiou, G.N.2    Morrison, I.E.3    Stevenson, G.V.4    Cherry, R.J.5
  • 3
    • 0034062812 scopus 로고    scopus 로고
    • H-ras but not K-ras traffics to the plasma membrane through the exocytic pathway
    • Apolloni, A., Prior, I. A., Lindsay, M., Parton, R. G. and Hancock, J. F. (2000). H-ras but not K-ras traffics to the plasma membrane through the exocytic pathway. Mol. Cell. Biol. 20, 2475-2487.
    • (2000) Mol. Cell. Biol. , vol.20 , pp. 2475-2487
    • Apolloni, A.1    Prior, I.A.2    Lindsay, M.3    Parton, R.G.4    Hancock, J.F.5
  • 4
    • 0037451803 scopus 로고    scopus 로고
    • GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila
    • Bernards, A. (2003). GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. Biochim. Biophys. Acta 1603, 47-82.
    • (2003) Biochim. Biophys. Acta , vol.1603 , pp. 47-82
    • Bernards, A.1
  • 5
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 6
    • 0026512314 scopus 로고
    • Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
    • Brown, D. A. and Rose, J. K. (1992). Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68, 533-544.
    • (1992) Cell , vol.68 , pp. 533-544
    • Brown, D.A.1    Rose, J.K.2
  • 8
    • 0028122478 scopus 로고
    • Ras-15A protein shares highly similar dominant-negative biological properties with Ras-17N and forms a stable, guanine-nucleotide resistant complex with CDC25 exchange factor
    • Chen, S. Y., Huff, S. Y., Lai, C. C., Der, C. J. and Powers, S. (1994). Ras-15A protein shares highly similar dominant-negative biological properties with Ras-17N and forms a stable, guanine-nucleotide resistant complex with CDC25 exchange factor. Oncogene 9, 2691-2698.
    • (1994) Oncogene , vol.9 , pp. 2691-2698
    • Chen, S.Y.1    Huff, S.Y.2    Lai, C.C.3    Der, C.J.4    Powers, S.5
  • 10
    • 0028228616 scopus 로고
    • Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells
    • Cowley, S., Paterson, H., Kemp, P. and Marshall, C. J. (1994). Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells. Cell 77, 841-852.
    • (1994) Cell , vol.77 , pp. 841-852
    • Cowley, S.1    Paterson, H.2    Kemp, P.3    Marshall, C.J.4
  • 11
    • 0142039980 scopus 로고    scopus 로고
    • Protein-tyrosine phosphatase 1B mediates the effects of insulin on the actin cytoskeleton in immortalized fibroblasts
    • Dadke, S. and Chernoff, J. (2003). Protein-tyrosine phosphatase 1B mediates the effects of insulin on the actin cytoskeleton in immortalized fibroblasts. J. Biol. Chem. 278, 40607-40611.
    • (2003) J. Biol. Chem. , vol.278 , pp. 40607-40611
    • Dadke, S.1    Chernoff, J.2
  • 12
    • 0036789949 scopus 로고    scopus 로고
    • Ras and relatives - Job sharing and networking keep an old family together
    • Ehrhardt, A., Ehrhardt, G. R., Guo, X. and Schrader, J. W. (2002). Ras and relatives - job sharing and networking keep an old family together. Exp. Hematol. 30, 1089-1106.
    • (2002) Exp. Hematol. , vol.30 , pp. 1089-1106
    • Ehrhardt, A.1    Ehrhardt, G.R.2    Guo, X.3    Schrader, J.W.4
  • 13
    • 0037020243 scopus 로고    scopus 로고
    • Galectin-1 augments Ras activation and diverts Ras signals to Raf-1 at the expense of phosphoinositide 3-kinase
    • Elad-Sfadia, G., Haklai, R., Ballan, E., Gabius, H. J. and Kloog, Y. (2002). Galectin-1 augments Ras activation and diverts Ras signals to Raf-1 at the expense of phosphoinositide 3-kinase. J. Biol. Chem. 277, 37169-37175.
    • (2002) J. Biol. Chem. , vol.277 , pp. 37169-37175
    • Elad-Sfadia, G.1    Haklai, R.2    Ballan, E.3    Gabius, H.J.4    Kloog, Y.5
  • 14
    • 0025940096 scopus 로고
    • Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP
    • Farnsworth, C. L. and Feig, L. A. (1991). Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP. Mol. Cell. Biol. 11, 4822-4829.
    • (1991) Mol. Cell. Biol. , vol.11 , pp. 4822-4829
    • Farnsworth, C.L.1    Feig, L.A.2
  • 15
    • 0023715225 scopus 로고
    • Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP
    • Feig, L. A. and Cooper, G. M. (1988). Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP. Mol. Cell. Biol. 8, 3235-3243.
    • (1988) Mol. Cell. Biol. , vol.8 , pp. 3235-3243
    • Feig, L.A.1    Cooper, G.M.2
  • 16
    • 0037054546 scopus 로고    scopus 로고
    • Phospholipids undergo hop diffusion in compartmentalized cell membrane
    • Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K. and Kusumi, A. (2002). Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157, 1071-1081.
    • (2002) J. Cell Biol. , vol.157 , pp. 1071-1081
    • Fujiwara, T.1    Ritchie, K.2    Murakoshi, H.3    Jacobson, K.4    Kusumi, A.5
  • 17
    • 0025013547 scopus 로고
    • A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane
    • Hancock, J. F., Paterson, H. and Marshall, C. J. (1990). A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane. Cell 63, 133-139.
    • (1990) Cell , vol.63 , pp. 133-139
    • Hancock, J.F.1    Paterson, H.2    Marshall, C.J.3
  • 18
    • 0026037624 scopus 로고
    • A CAAX or a CAAL motif and a second signal are sufficient for plasma membrane targeting of ras proteins
    • Hancock, J. F., Cadwallader, K., Paterson, H. and Marshall, C. J. (1991). A CAAX or a CAAL motif and a second signal are sufficient for plasma membrane targeting of ras proteins. EMBO J. 10, 4033-4039.
    • (1991) EMBO J. , vol.10 , pp. 4033-4039
    • Hancock, J.F.1    Cadwallader, K.2    Paterson, H.3    Marshall, C.J.4
  • 19
    • 0035033890 scopus 로고    scopus 로고
    • Autofluorescent proteins in single-molecule research: Applications to live cell imaging microscopy
    • Harms, G. S., Cognet, L., Lommerse, P. H. M., Blab, G. A. and Schmidt, T. (2001). Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy. Biophys. J. 80, 2396-2408.
    • (2001) Biophys. J. , vol.80 , pp. 2396-2408
    • Harms, G.S.1    Cognet, L.2    Lommerse, P.H.M.3    Blab, G.A.4    Schmidt, T.5
  • 20
    • 0038788003 scopus 로고    scopus 로고
    • Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging
    • Ike, H., Kosugi, A., Kato, A., Iino, R., Hirano, H., Fujiwara, T., Ritchie, K. and Kusumi, A. (2003). Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging. Chem. Phys. Chem. 4, 620-626.
    • (2003) Chem. Phys. Chem. , vol.4 , pp. 620-626
    • Ike, H.1    Kosugi, A.2    Kato, A.3    Iino, R.4    Hirano, H.5    Fujiwara, T.6    Ritchie, K.7    Kusumi, A.8
  • 22
    • 0033962410 scopus 로고    scopus 로고
    • Insulin-induced actin filament remodeling colocalizes actin with phosphatidylinositol 3-kinase and GLUT4 in L6 myotubes
    • Khayat, Z. A., Tong, P., Yaworsky, K., Bloch, R. J. and Klip, A. (2000). Insulin-induced actin filament remodeling colocalizes actin with phosphatidylinositol 3-kinase and GLUT4 in L6 myotubes. J. Cell Sci. 113, 279-290.
    • (2000) J. Cell Sci. , vol.113 , pp. 279-290
    • Khayat, Z.A.1    Tong, P.2    Yaworsky, K.3    Bloch, R.J.4    Klip, A.5
  • 23
    • 0027504198 scopus 로고
    • Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells
    • Kusumi, A., Sako, Y. and Yamamoto, M. (1993). Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65, 2021-2040.
    • (1993) Biophys. J. , vol.65 , pp. 2021-2040
    • Kusumi, A.1    Sako, Y.2    Yamamoto, M.3
  • 24
    • 0027193913 scopus 로고
    • Insulin-induced circular membrane ruffling on rat 1 cells expressing a high number of human insulin receptors: Circular ruffles caused by rapid actin reorganization exhibit high density of insulin receptors and phosphotyrosines
    • Li, S. L., Miyata, Y., Yahara, I. and Fujita-Yamaguchi, Y. (1993). Insulin-induced circular membrane ruffling on rat 1 cells expressing a high number of human insulin receptors: circular ruffles caused by rapid actin reorganization exhibit high density of insulin receptors and phosphotyrosines. Exp. Cell Res. 205, 353-360.
    • (1993) Exp. Cell Res. , vol.205 , pp. 353-360
    • Li, S.L.1    Miyata, Y.2    Yahara, I.3    Fujita-Yamaguchi, Y.4
  • 25
    • 0346057912 scopus 로고    scopus 로고
    • Single-molecule imaging of the H-Ras membrane-anchor reveals domains in the cytoplasmic leaflet of the cell membrane
    • Lommerse, P. H. M., Blab, G. A., Cognet, L., Harms, G. S., Snaar-Jagalska, B. E., Spaink, H. P. and Schmidt, T. (2004). Single-molecule imaging of the H-Ras membrane-anchor reveals domains in the cytoplasmic leaflet of the cell membrane. Biophys. J. 86, 609-616.
    • (2004) Biophys. J. , vol.86 , pp. 609-616
    • Lommerse, P.H.M.1    Blab, G.A.2    Cognet, L.3    Harms, G.S.4    Snaar-Jagalska, B.E.5    Spaink, H.P.6    Schmidt, T.7
  • 27
    • 0037182579 scopus 로고    scopus 로고
    • Activated K-Ras and H-Ras display different interactions with saturable nonraft sites at the surface of live cells
    • Niv, H., Gutman, O., Kloog, Y. and Henis, Y. I. (2002). Activated K-Ras and H-Ras display different interactions with saturable nonraft sites at the surface of live cells. J. Cell Biol. 157, 865-872.
    • (2002) J. Cell Biol. , vol.157 , pp. 865-872
    • Niv, H.1    Gutman, O.2    Kloog, Y.3    Henis, Y.I.4
  • 28
    • 0037445938 scopus 로고    scopus 로고
    • Imaging metabolism of phosphatidylinositol 4,5-bisphosphate in T-cell GM1-enriched domains containing Ras proteins
    • Parmryd, I., Adler, J., Patel, R. and Magee, A. I. (2003). Imaging metabolism of phosphatidylinositol 4,5-bisphosphate in T-cell GM1-enriched domains containing Ras proteins. Exp. Cell Res. 285, 27-38.
    • (2003) Exp. Cell Res. , vol.285 , pp. 27-38
    • Parmryd, I.1    Adler, J.2    Patel, R.3    Magee, A.I.4
  • 30
    • 0035067187 scopus 로고    scopus 로고
    • GTP-dependent segregation of H-ras from lipid rafts is required for biological activity
    • Prior, I. A., Harding, A., Yan, J., Sluimer, J., Parton, R. G. and Hancock, J. F. (2001). GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat. Cell Biol. 3, 368-375.
    • (2001) Nat. Cell Biol. , vol.3 , pp. 368-375
    • Prior, I.A.1    Harding, A.2    Yan, J.3    Sluimer, J.4    Parton, R.G.5    Hancock, J.F.6
  • 31
    • 0037455589 scopus 로고    scopus 로고
    • Direct visualization of Ras proteins in spatially distinct cell surface microdomains
    • Prior, I. A., Muncke, C., Parton, R. G. and Hancock, J. F. (2003). Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J. Cell Biol. 160, 165-170.
    • (2003) J. Cell Biol. , vol.160 , pp. 165-170
    • Prior, I.A.1    Muncke, C.2    Parton, R.G.3    Hancock, J.F.4
  • 32
    • 0036364408 scopus 로고    scopus 로고
    • A growing family of guanine nucleotide exchange factors is responsible for activation of Ras-family GTPases
    • Quilliam, L. A., Rebhun, J. F. and Castro, A. F. (2002). A growing family of guanine nucleotide exchange factors is responsible for activation of Ras-family GTPases. Prog. Nucleic Acid Res. Mol. Biol. 71, 391-444.
    • (2002) Prog. Nucleic Acid Res. Mol. Biol. , vol.71 , pp. 391-444
    • Quilliam, L.A.1    Rebhun, J.F.2    Castro, A.F.3
  • 33
    • 3242657115 scopus 로고    scopus 로고
    • Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane
    • Rotblat, B., Prior, I. A., Muncke, C., Parton, R. G., Kloog, Y., Henis, Y. I. and Hancock, J. F. (2004). Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol. Cell. Biol. 24, 6799-6810.
    • (2004) Mol. Cell. Biol. , vol.24 , pp. 6799-6810
    • Rotblat, B.1    Prior, I.A.2    Muncke, C.3    Parton, R.G.4    Kloog, Y.5    Henis, Y.I.6    Hancock, J.F.7
  • 34
    • 0030050141 scopus 로고    scopus 로고
    • Anomalous diffusion due to binding: A Monte Carlo study
    • Saxton, M. J. (1996). Anomalous diffusion due to binding: a Monte Carlo study. Biophys. J. 70, 1250-1262.
    • (1996) Biophys. J. , vol.70 , pp. 1250-1262
    • Saxton, M.J.1
  • 35
    • 33750626346 scopus 로고
    • Characterization of photophysics and mobility of single molecules in a fluid lipid membrane
    • Schmidt, T., Schütz, G. J., Baumgartner, W., Gruber, H. J. and Schindler, H. (1995). Characterization of photophysics and mobility of single molecules in a fluid lipid membrane. J. Phys. Chem. 99, 17662-17668.
    • (1995) J. Phys. Chem. , vol.99 , pp. 17662-17668
    • Schmidt, T.1    Schütz, G.J.2    Baumgartner, W.3    Gruber, H.J.4    Schindler, H.5
  • 37
    • 0039587949 scopus 로고    scopus 로고
    • Single-molecule microscopy on model membranes reveals anomalous diffusion
    • Schütz, G. J., Schindler, H. and Schmidt, T. (1997). Single-molecule microscopy on model membranes reveals anomalous diffusion. Biophys. J. 73, 1073-1080.
    • (1997) Biophys. J. , vol.73 , pp. 1073-1080
    • Schütz, G.J.1    Schindler, H.2    Schmidt, T.3
  • 38
    • 0021126650 scopus 로고
    • Biological properties of human c-Ha-ras1 genes mutated at codon 12
    • Seeburg, P. H., Colby, W. W., Capon, D. J., Goedel, D. V. and Levinson, A. D. (1984). Biological properties of human c-Ha-ras1 genes mutated at codon 12. Nature 312, 71-75.
    • (1984) Nature , vol.312 , pp. 71-75
    • Seeburg, P.H.1    Colby, W.W.2    Capon, D.J.3    Goedel, D.V.4    Levinson, A.D.5
  • 39
    • 0030949124 scopus 로고    scopus 로고
    • Functional rafts in cell membranes
    • Simons, K. and Ikonen, E. (1997). Functional rafts in cell membranes. Nature 387, 569-572.
    • (1997) Nature , vol.387 , pp. 569-572
    • Simons, K.1    Ikonen, E.2
  • 40
    • 7044226349 scopus 로고    scopus 로고
    • Structural analysis of autoinhibition in the Ras activator Son of sevenless
    • Sondermann, H., Soisson, S. M., Boykevisch, S., Yang, S. S., Bar-Sagi, D. and Kuriyan, J. (2004). Structural analysis of autoinhibition in the Ras activator Son of sevenless. Cell 119, 393-405.
    • (2004) Cell , vol.119 , pp. 393-405
    • Sondermann, H.1    Soisson, S.M.2    Boykevisch, S.3    Yang, S.S.4    Bar-Sagi, D.5    Kuriyan, J.6
  • 41
    • 0023766145 scopus 로고
    • Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II
    • Sturgill, T. W., Ray, L. B., Erikson, E. and Maller, J. L. (1988). Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II. Nature 334, 715-718.
    • (1988) Nature , vol.334 , pp. 715-718
    • Sturgill, T.W.1    Ray, L.B.2    Erikson, E.3    Maller, J.L.4
  • 42
    • 0037046799 scopus 로고    scopus 로고
    • Activation of phosphoinositide 3-kinase gamma by Ras
    • Suire, S., Hawkins, P. and Stephens, L. (2002). Activation of phosphoinositide 3-kinase gamma by Ras. Curr. Biol. 12, 1068-1075.
    • (2002) Curr. Biol. , vol.12 , pp. 1068-1075
    • Suire, S.1    Hawkins, P.2    Stephens, L.3
  • 43
    • 0032561465 scopus 로고    scopus 로고
    • Actin filaments facilitate insulin activation of the src and collagen homologous/mitogen-activated protein kinase pathway leading to DNA synthesis and c-fos expression
    • Tsakiridis, T., Bergman, A., Somwar, R., Taha, C., Aktories, K., Cruz, T. F., Klip, A. and Downey, G. P. (1998). Actin filaments facilitate insulin activation of the src and collagen homologous/mitogen-activated protein kinase pathway leading to DNA synthesis and c-fos expression. J. Biol. Chem. 273, 28322-28331.
    • (1998) J. Biol. Chem. , vol.273 , pp. 28322-28331
    • Tsakiridis, T.1    Bergman, A.2    Somwar, R.3    Taha, C.4    Aktories, K.5    Cruz, T.F.6    Klip, A.7    Downey, G.P.8
  • 45
    • 0023671841 scopus 로고
    • Lipid polarity and sorting in epithelial cells
    • van Meer, G. and Simons, K. (1988). Lipid polarity and sorting in epithelial cells. J. Cell Biochem. 36, 51-58.
    • (1988) J. Cell Biochem. , vol.36 , pp. 51-58
    • van Meer, G.1    Simons, K.2
  • 46
    • 0033546419 scopus 로고    scopus 로고
    • Four human ras homologs differ in their abilities to activate Raf-1, induce transformation, and stimulate cell motility
    • Voice, J. K., Klemke, R. L., Le, A. and Jackson, J. H. (1999). Four human ras homologs differ in their abilities to activate Raf-1, induce transformation, and stimulate cell motility. J. Biol. Chem. 274, 17164-17170.
    • (1999) J. Biol. Chem. , vol.274 , pp. 17164-17170
    • Voice, J.K.1    Klemke, R.L.2    Le, A.3    Jackson, J.H.4
  • 47
    • 0021170342 scopus 로고
    • The p21 ras C-terminus is required for transformation and membrane association
    • Willumsen, B. M., Christensen, A., Hubbert, N. L., Papageorge, A. G. and Lowy, D. R. (1984). The p21 ras C-terminus is required for transformation and membrane association. Nature 310, 583-586.
    • (1984) Nature , vol.310 , pp. 583-586
    • Willumsen, B.M.1    Christensen, A.2    Hubbert, N.L.3    Papageorge, A.G.4    Lowy, D.R.5
  • 48
    • 0032508517 scopus 로고    scopus 로고
    • Ras isoforms vary in their ability to activate Raf-1 and phosphoinositide 3-kinase
    • Yan, J., Roy, S., Apolloni, A., Lane, A. and Hancock, J. F. (1998). Ras isoforms vary in their ability to activate Raf-1 and phosphoinositide 3-kinase. J. Biol. Chem. 273, 24052-24056.
    • (1998) J. Biol. Chem. , vol.273 , pp. 24052-24056
    • Yan, J.1    Roy, S.2    Apolloni, A.3    Lane, A.4    Hancock, J.F.5
  • 49
    • 0037012847 scopus 로고    scopus 로고
    • Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    • Zacharias, D. A., Violin, J. D., Newton, A. C. and Tsien, R. Y. (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-916.
    • (2002) Science , vol.296 , pp. 913-916
    • Zacharias, D.A.1    Violin, J.D.2    Newton, A.C.3    Tsien, R.Y.4


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