Development of pyrF-Based genetic system for targeted gene deletion in clostridium thermocellum and creation of a pta mutant

Applied and Environmental Microbiology

Volumn 76, Issue 19, 2010, Pages 6591-6599

  Tripathi, Shital A ^a,c   Olson, Daniel G ^a,b,c   Argyros, D Aaron ^a,c   Miller, Bethany B ^a,c   Barrett, Trisha F ^a,c   Murphy, Daniel M ^a,c   McCool, Jesse D ^d   Warner, Anne K ^a,c   Rajgarhia, Vineet B ^e   Lynd, Lee R ^a,b,c   Hogsett, David A ^a,c   Caiazza, Nicky C ^a,c  

^a Mascoma Corporation   (United States)

^b DARTMOUTH COLLEGE   (United States)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

^d LONZA AG   (Switzerland)

^e Bio Architecture Lab   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID PRODUCTION; CLOSTRIDIUM THERMOCELLUM; ECTOPIC EXPRESSIONS; GENE DELETION; GENE KNOCKOUT; GENETIC SELECTION; GENETIC SYSTEMS; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; NEGATIVE SELECTION; PHOSPHOTRANSACETYLASE; SUSTAINABLE ENERGY; THERMOPHILIC ANAEROBIC BACTERIA;

CLOSTRIDIUM; ENERGY RESOURCES; MICROBIOLOGY; ORGANIC ACIDS;

GENES;

5-FLUOROOROTIC ACID; BACTERIAL PROTEIN; CARBOXYLIC ACID; DRUG DERIVATIVE; FLUOROOROTIC ACID; OROTIC ACID; PHOSPHATE ACETYLTRANSFERASE;

ALTERNATIVE ENERGY; ANOXIC CONDITIONS; BIOFUEL; CELLULOSE; ENZYME ACTIVITY; GENE; METABOLISM; MUTATION; PLASMID; RECOMBINATION; RENEWABLE RESOURCE; SOLUBILIZATION; SUSTAINABILITY; THERMOPHILIC BACTERIUM;

ARTICLE; CLOSTRIDIUM THERMOCELLUM; GENE DELETION; GENE INACTIVATION; GENETIC SELECTION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; PLASMID;

BACTERIAL PROTEINS; CARBOXYLIC ACIDS; CLOSTRIDIUM THERMOCELLUM; GENE DELETION; GENE KNOCKOUT TECHNIQUES; METABOLIC NETWORKS AND PATHWAYS; MOLECULAR BIOLOGY; OROTIC ACID; PHOSPHATE ACETYLTRANSFERASE; PLASMIDS; SELECTION, GENETIC;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM;

EID: 78049278436     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01484-10     Document Type: Article

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