Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1437-1445

  Gao, Min Jie ^a   Zheng, Zhi Yong ^a   Wu, Jian Rong ^a   Dong, Shi Juan ^b   Li, Zhen ^b   Jin, Hu ^a   Zhan, Xiao Bei ^a   Lin, Chi Chung ^a  

^a JIANGNAN UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Artificial neural network; DO stat; Fed batch culture; Pichia pastoris; Protein expression

Indexed keywords

CELL DENSITY; DO-STAT; DRY CELLS; FED-BATCH CULTURES; FEEDING SCHEME; FEEDING STRATEGIES; FERMENTORS; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PRODUCTION; SPECIFIC GROWTH RATE; TARGET PROTEINS;

ANTIBIOTICS; BATCH CELL CULTURE; GLYCEROL; GLYCOPROTEINS; GROWTH RATE; NEURAL NETWORKS; PATTERN RECOGNITION; PROTEINS; YEAST;

FEEDING;

ALPHA INTERFERON; GLYCEROL;

ARTIFICIAL NEURAL NETWORK; BIOREACTOR; DISSOLVED OXYGEN; GENE EXPRESSION; GROWTH RATE; PROTEIN; RECOMBINATION; YEAST;

ARTICLE; ARTIFICIAL NEURAL NETWORK; CELL DENSITY; CELL GROWTH; CYTOKINE PRODUCTION; FEEDING; FUNGUS GROWTH; NONHUMAN; NUCLEOTIDE SEQUENCE; ONLINE SYSTEM; PICHIA PASTORIS;

ANIMALS; BIOTECHNOLOGY; CULTURE MEDIA; FERMENTATION; GLYCEROL; INTERFERON-ALPHA; NEURAL NETWORKS (COMPUTER); PICHIA; RECOMBINANT PROTEINS; SWINE;

PICHIA PASTORIS; SUS;

EID: 84857919366     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3605-8     Document Type: Article

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