Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation

Metabolic Engineering

Volumn 12, Issue 1, 2010, Pages 8-17

  Schroer, Kirsten ^a   Peter Luef, Klaus ^a   Stefan Hartner, Franz ^a,b   Glieder, Anton ^a,c   Pscheidt, Beate ^a  

^a RESEARCH CENTRE APPLIED BIOCATALYSIS   (Austria)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Alcohol oxidase; Cofactor regeneration; Formaldehyde dehydrogenase; Formate dehydrogenase; Methanol pathway; Pathway engineering; Pichia pastoris; Whole cell biocatalysis

Indexed keywords

ALCOHOL OXIDASE; COFACTOR REGENERATION; FORMALDEHYDE DEHYDROGENASE; FORMATE DEHYDROGENASE; PATHWAY ENGINEERING; PICHIA PASTORIS; WHOLE-CELL BIOCATALYSIS;

DISCRIMINANT ANALYSIS; FORMALDEHYDE; INDUSTRIAL CHEMICALS; METHANOL; OXIDATION;

ENZYMES;

ALCOHOL OXIDASE; BUTANEDIOL; FORMALDEHYDE DEHYDROGENASE; METHANOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BACTERIAL CELL; BIOTRANSFORMATION; CONTROLLED STUDY; FUNGAL STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

ALCOHOL OXIDOREDUCTASES; ALDEHYDE OXIDOREDUCTASES; FORMATE DEHYDROGENASES; FUNGAL PROTEINS; GENETIC ENGINEERING; KINETICS; METHANOL; NAD; OXIDATION-REDUCTION; PICHIA;

PICHIA PASTORIS;

EID: 70449530436     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2009.08.006     Document Type: Article

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