WS6 induces both alpha and beta cell proliferation without affecting differentiation or viability

Endocrine Journal

Volumn 62, Issue 4, 2015, Pages 379-386

  Boerner, Brian P ^a,b   George, Nicholas M ^a,b   Mir, Shakeel U R ^a,b   Sarvetnick, Nora E ^a,b  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Alpha cell; Differentiation; Human islet

Indexed keywords

INSULIN; MITOGENIC AGENT; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; WS6; CARBANILAMIDE DERIVATIVE; WS6 COMPOUND;

ADULT; APOPTOSIS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STIMULATION; CELL VIABILITY; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN CELL; MALE; MIDDLE AGED; NOTE; PANCREAS ISLET ALPHA CELL; PANCREAS ISLET BETA CELL; PHENOTYPE; PROTEIN EXPRESSION; YOUNG ADULT; CELL CULTURE; CELL SURVIVAL; DRUG EFFECTS; PHYSIOLOGY; UPREGULATION;

ADULT; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; FEMALE; GLUCAGON-SECRETING CELLS; HUMANS; INSULIN-SECRETING CELLS; MALE; MIDDLE AGED; MITOGENS; PHENYLUREA COMPOUNDS; UP-REGULATION; YOUNG ADULT;

EID: 84929470943     PISSN: 09188959     EISSN: 13484540     Source Type: Journal    
DOI: 10.1507/endocrj.EJ14-0449     Document Type: Note

References (30)

1. Bernal-Mizrachi E, Kulkarni RN, Scott DK, Mauvais-Jarvis F, Stewart AF, et al. (2014) Human beta-cell proliferation and intracellular signaling part 2: Still driving in the dark without a road map. Diabetes 63: 819-831.
2. Jiao Y, Le Lay J, Yu M, Naji A, Kaestner KH (2014) Elevated mouse hepatic betatrophin expression does not increase human beta-cell replication in the transplant setting. Diabetes 63: 1283-1288.
3. Fiaschi-Taesch NM, Salim F, Kleinberger J, Troxell R, Cozar-Castellano I, et al. (2010) Induction of human beta-cell proliferation and engraftment using a single G1/S regulatory molecule, cdk6. Diabetes 59: 1926-1936.
4. Rady B, Chen Y, Vaca P, Wang Q, Wang Y, et al. (2013) Overexpression of E2F3 promotes proliferation of functional human beta cells without induction of apoptosis. Cell Cycle 12: 2691-2702.
5. Guthalu Kondegowda N, Joshi-Gokhale S, Harb G, Williams K, Zhang XY, et al. (2010) Parathyroid hormone-related protein enhances human beta-cell proliferation and function with associated induction of cyclin-dependent kinase 2 and cyclin E expression. Diabetes 59: 3131-3138.
6. Boerner BP, George NM, Targy NM, Sarvetnick NE (2013) TGF-beta superfamily member nodal stimulates human beta-cell proliferation while maintaining cellular viability. Endocrinology 154: 4099-4112.
7. Shen W, Tremblay MS, Deshmukh VA, Wang W, Filippi CM, et al. (2013) Small-molecule inducer of beta cell proliferation identified by high-throughput screening. J Am Chem Soc 135: 1669-1672.
8. Taylor BL, Liu FF, Sander M (2013) Nkx6.1 is essential for maintaining the functional state of pancreatic beta cells. Cell Rep 4: 1262-1275.
9. Guo S, Dai C, Guo M, Taylor B, Harmon JS, et al. (2013) Inactivation of specific beta cell transcription factors in type 2 diabetes. J Clin Invest 123: 3305-3316.
10. Talchai C, Xuan S, Lin HV, Sussel L, Accili D (2012) Pancreatic beta cell dedifferentiation as a mechanism of diabetic beta cell failure. Cell 150: 1223-1234.
11. Ellingsgaard H, Ehses JA, Hammar EB, Van Lommel L, Quintens R, et al. (2008) Interleukin-6 regulates pancreatic alpha-cell mass expansion. Proc Natl Acad Sci U S A 105: 13163-13168.
12. Liu Z, Kim W, Chen Z, Shin YK, Carlson OD, et al. (2011) Insulin and glucagon regulate pancreatic alpha-cell proliferation. PLoS One 6: e16096.
13. Hayes HL, Moss LG, Schisler JC, Haldeman JM, Zhang Z, et al. (2013) Pdx-1 activates islet alpha- and beta-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol 33: 4017-4029.
14. Dor Y, Brown J, Martinez OI, Melton DA (2004) Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429: 41-46.
15. Szabat M, Johnson JD, Piret JM (2010) Reciprocal modulation of adult beta cell maturity by activin A and follistatin. Diabetologia 53: 1680-1689.
16. Brissova M, Shiota M, Nicholson WE, Gannon M, Knobel SM, et al. (2002) Reduction in pancreatic transcription factor PDX-1 impairs glucose-stimulated insulin secretion. J Biol Chem 277: 11225-11232.
17. Schaffer AE, Taylor BL, Benthuysen JR, Liu J, Thorel F, et al. (2013) Nkx6.1 controls a gene regulatory network required for establishing and maintaining pancreatic beta cell identity. PLoS Genet 9: e1003274.
18. Walpita D, Hasaka T, Spoonamore J, Vetere A, Takane KK, et al. (2012) A human islet cell culture system for high-throughput screening. J Biomol Screen 17: 509-518.
19. Wang W, Walker JR, Wang X, Tremblay MS, Lee JW, et al. (2009) Identification of small-molecule inducers of pancreatic beta-cell expansion. Proc Natl Acad Sci U S A 106: 1427-1432.
20. Annes JP, Ryu JH, Lam K, Carolan PJ, Utz K, et al. (2012) Adenosine kinase inhibition selectively promotes rodent and porcine islet beta-cell replication. Proc Natl Acad Sci U S A 109: 3915-3920.
21. Vetere A, Wagner BK (2012) Chemical methods to induce beta-cell proliferation. Int J Endocrinol 2012: 925143.
22. Henquin JC, Rahier J (2011) Pancreatic alpha cell mass in European subjects with type 2 diabetes. Diabetologia 54: 1720-1725.
23. Guardado-Mendoza R, Davalli AM, Chavez AO, Hubbard GB, Dick EJ, et al. (2009) Pancreatic islet amyloidosis, beta-cell apoptosis, and alpha-cell proliferation are determinants of islet remodeling in type-2 diabetic baboons. Proc Natl Acad Sci U S A 106: 13992-13997.
24. Unger RH, Aguilar-Parada E, Muller WA, Eisentraut AM (1970) Studies of pancreatic alpha cell function in normal and diabetic subjects. J Clin Invest 49: 837-848.
25. Sherwin RS, Fisher M, Hendler R, Felig P (1976) Hyperglucagonemia and blood glucose regulation in normal, obese and diabetic subjects. N Engl J Med 294: 455-461.
26. Yoon KH, Ko SH, Cho JH, Lee JM, Ahn YB, et al. (2003) Selective beta-cell loss and alpha-cell expansion in patients with type 2 diabetes mellitus in korea. J Clin Endocrinol Metab 88: 2300-2308.
27. Russ HA, Bar Y, Ravassard P, Efrat S (2008) In vitro proliferation of cells derived from adult human beta-cells revealed by cell-lineage tracing. Diabetes 57: 1575-1583.
28. El-Gohary Y, Tulachan S, Wiersch J, Guo P, Welsh C, et al. (2014) A smad signaling network regulates islet cell proliferation. Diabetes 63: 224-236.
29. Kulkarni RN, Jhala US, Winnay JN, Krajewski S, Montminy M, et al. (2004) PDX-1 haploinsufficiency limits the compensatory islet hyperplasia that occurs in response to insulin resistance. J Clin Invest 114: 828-836.
30. Gu C, Stein GH, Pan N, Goebbels S, Hornberg H, et al. (2010) Pancreatic beta cells require NeuroD to achieve and maintain functional maturity. Cell Metab 11: 298-310.