rational design and directed evolution of CYP102A1 (BM3) for regio-specific hydroxylation of isoflavone

Biotechnology and Bioprocess Engineering

Volumn 20, Issue 2, 2015, Pages 225-233

  Ko, Sungghi ^a   Yang, Yung Hun ^b   Choi, Kwon Young ^c   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

^b Konkuk University   (South Korea)

^c Ajou University   (South Korea)

Author keywords

cytochrome P450 monooxygenases; isoflavone; ortho specific hydroxylation; regioselectivity; self sufficient CYP102A1

Indexed keywords

AROMATIC HYDROCARBONS; ENZYMES; FLAVONOIDS; METABOLITES; POLYCYCLIC AROMATIC HYDROCARBONS; REGIOSELECTIVITY;

ANTIOXIDANT EFFECT; CYTOCHROME P450 ENZYMES; CYTOCHROME P450 MONOOXYGENASES; DIRECTED EVOLUTION; ENZYME ENGINEERING; ISOFLAVONES; SECONDARY METABOLITES; SELF-SUFFICIENT CYP102A1;

HYDROXYLATION;

7 ETHOXYCOUMARIN; APIGENIN; CHRYSIN; CONIFERYL ALCOHOL; COUMARIN; CURCUMIN; CYTOCHROME; CYTOCHROME P102 A7; CYTOCHROME P450 BM3; DAIDZEIN; ISOFLAVONE DERIVATIVE; ISOFLAVONOID; ORTHO DIHYDROXYISOFLAVONE; PHLORETIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION KINETICS; ENZYME ACTIVE SITE; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR MODEL; NONHUMAN;

EID: 84929463544     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-014-0718-9     Document Type: Article

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