Engineering class I cytochrome P450 by gene fusion with NADPH-dependent reductase and S. avermitilis host development for daidzein biotransformation

Applied Microbiology and Biotechnology

Volumn 98, Issue 19, 2014, Pages 8191-8200

  Choi, Kwon Young ^a   Jung, Eun Ok ^b   Yun, Hyungdon ^c   Yang, Yung Hun ^c   Kim, Byung Gee ^b  

^a Ajou University   (South Korea)

^b Seoul National University   (South Korea)

^c Konkuk University   (South Korea)

Author keywords

Cytochrome P450; Daidzein; Fusion enzyme; Redox partner; S. avermitilis host

Indexed keywords

CATALYSIS; EFFICIENCY; ELECTRON TRANSITIONS; ENZYMES; HYDROXYLATION; PORPHYRINS;

CYTOCHROME P450; DAIDZEIN; FUSION ENZYMES; REDOX PARTNERS; S. AVERMITILIS HOST;

FLAVONOIDS;

BACTERIAL PROTEIN; DAIDZEIN; HYBRID PROTEIN; ISOFLAVONE DERIVATIVE; MIXED FUNCTION OXIDASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

BIOTRANSFORMATION; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLIC ENGINEERING; METABOLISM; NOCARDIA; STREPTOMYCES;

BACTERIAL PROTEINS; BIOTRANSFORMATION; ISOFLAVONES; KINETICS; METABOLIC ENGINEERING; MIXED FUNCTION OXYGENASES; NADPH-FERRIHEMOPROTEIN REDUCTASE; NOCARDIA; RECOMBINANT FUSION PROTEINS; STREPTOMYCES;

EID: 84920253529     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5706-7     Document Type: Article

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