Effect of lipopolysaccharide mutation on oxygenation of linoleic acid by recombinant Escherichia coli expressing CYP102A2 of Bacillus subtilis

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 1, 2011, Pages 7-12

  Lee, Na Rae ^a   Yoon, Jang Won ^a   Park, Jin Byung ^a  

^a Ewha Womans University   (South Korea)

Author keywords

Biocatalysis; CYP102A2; Escherichia coli; Lipopolysaccharide; Substrate transport

Indexed keywords

BACILLUS SUBTILIS; BIOCATALYSIS; CELL WALLS; CYP102A2; E. COLI; HYDROPHOBIC MOLECULES; LIPID A; LIPOPOLYSACCHARIDES; MONOOXYGENASES; MUTANT STRAIN; ORGANIC SUBSTRATE; RECOMBINANT ESCHERICHIA COLI; SPECIFIC ACTIVITY; WHOLE-CELL BIOTRANSFORMATIONS; WILD TYPES;

BACILLI; BACTERIOLOGY; BIOCONVERSION; CELLS; ENZYMES; ESCHERICHIA COLI; GENE ENCODING; OXYGENATION; OXYGENATORS; SUBSTRATES; UNSATURATED FATTY ACIDS;

LINOLEIC ACID;

BACTERIUM LIPOPOLYSACCHARIDE; LINOLEIC ACID; LIPID A;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL WALL; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM MUTANT; BIOCATALYST; BIOENGINEERING; BIOTRANSFORMATION; CARBOHYDRATE ANALYSIS; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; CYTOCHROME P450 102A2 GENE; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE OVEREXPRESSION; GENETIC CODE; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; HYDROPHOBICITY; LIPOPHILICITY; MUTATIONAL ANALYSIS; NONHUMAN; OXYGENATION; WILD TYPE;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 84863012170     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-010-0243-4     Document Type: Article

References (25)

1. Blank, L. M., B. E. Ebert, K. Buehler, and B. Buhler (2010) Redox biocatalysis and metabolism: Molecular mechanisms and metabolic network analysis. Antioxid. Redox Signal. 13:349-394.
2. Munro, A. W., H. M. Girvan, and K. J. McLean (2007) Variations on a (t) heme - novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily. Nat. Prod. Rep. 24:585-609. (Pubitemid 46848711)
3. Hannemann, F., A. Bichet, K. M. Ewen, and R. Bernhardt (2007) Cytochrome P450 systems - biological variations of electron transport chains. Biochim. Biophys. Acta-Gen. Subj. 1770:330-344. (Pubitemid 46157074)
4. Hilker, B. L., H. Fukushige, C. Hou, and D. Hildebrand (2008) Comparison of Bacillus monooxygenase genes for unique fatty acid production. Prog. Lipid Res. 47:1-14. (Pubitemid 350216619)
5. Dietrich, M., S. Eiben, C. Asta, T. A. Do, J. Pleiss, and V. B. Urlacher (2008) Cloning, expression and characterisation of CYP102A7, a self-sufficient P450 monooxygenase from Bacillus licheniformis. Appl. Microbiol. Biotechnol. 79:931-940.
6. Lentz, O., V. Urlacher, and R. D. Schmid (2004) Substrate specificity of native and mutated cytochrome P450 (CYP102A3) from Bacillus subtilis. J. Biotechnol. 108:41-49. (Pubitemid 38115139)
7. Capdevila, J. H., S. Wei, C. Helvig, J. R. Falck, Y. Belosludtsev, G. Truan, S. E. Graham-Lorence, and J. A. Peterson (1996) The highly stereoselective oxidation of polyunsaturated fatty acids by cytochrome P450 BM-3. J. Biol. Chem. 271:22663-22671. (Pubitemid 26304709)
8. Lentz, O., A. Feenstra, T. Habicher, B. Hauer, R. D. Schmid, and V. B. Urlacher (2006) Altering the regioselectivity of cytochrome P450CYP102A3 of Bacillus subtilis by using a new versatile assay system. ChemBioChem. 7:345-350. (Pubitemid 43220949)
9. Whitehouse, C. J. C., S. G. Bell, H. G. Tufton, R. J. P. Kenny, L. C. I. Ogilvie, and L. L. Wong (2008) Evolved CYP102A1 (P450 (BM3)) variants oxidise a range of non-natural substrates and offer new selectivity options. Chem. Commun. 966-968. (Pubitemid 351281049)
10. Glieder, A., E. T. Farinas, and F. H. Arnold (2002) Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nat. Biotechnol. 20:1135-1139. (Pubitemid 35285443)
11. Peters, M. W., P. Meinhold, A. Glieder, and F. H. Arnold (2003) Regio-and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3. J. Am. Chem. Soc. 125:13442-13450. (Pubitemid 37352128)
12. Park, J. B. (2007) Oxygenase-based whole-cell biocatalysis in organic synthesis. J. Microbiol. Biotechnol. 17:379-392.
13. Doo, E. H., W. H. Lee, H. S. Seo, J. H. Seo, and J. B. Park (2009) Productivity of cyclohexanone oxidation of the recombinant Corynebacterium glutamicum expressing chnB of Acinetobacter calcoaceticus. J. Biotechnol. 142:164-169.
14. Pandey, B. P., C. Roh, K. Y. Choi, N. Lee, E. J. Kim, S. Ko, T. Kim, H. Yun, and B. G. Kim (2010) Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680. Biotechnol. Bioeng. 105:697-704.
15. Bae, J.-W., E.-H. Doo, S.-H. Shin, S.-G. Lee, Y.-J. Jeong, J.-B. Park, and S. Park (2009) Development of a recombinant Escherichia coli-based biocatalyst to enable high styrene epoxidation activity with high product yield on energy source. Proc. Biochem. 45:147-152.
16. Julsing, M. K., S. Cornelissen, B. Buhler, and A. Schmid (2008) Heme-iron oxygenases: Powerful industrial biocatalysts?. Cur. Opin. Chem. Biol. 12:177-186.
17. Rosic, N. N. (2009) Versatile capacity of shuffled cytochrome P450s for dye production. Appl. Microbiol. Biotechnol. 82:203-210.
18. Schneider, S., M. G. Wubbolts, D. Sanglard, and B. Witholt (1998) Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for in vivo application of cytochrome P-450 (BM-3) monooxygenase. Appl. Environ. Microbiol. 64:3784-3790. (Pubitemid 28462973)
19. Ni, Y. and R. R. Chen (2005) Lipoprotein mutation accelerates substrate permeability-limited toluene dioxygenase-catalyzed reaction. Biotechnol. Prog. 21:799-805. (Pubitemid 40772285)
20. Riesenberg, D. (1991) High-cell-density cultivation of Escherichia coli. Cur. Opin. Biotechnol. 2:380-384.
21. Budde, M., S. C. Maurer, R. D. Schmid, and V. B. Urlacher (2004) Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtilis. Appl. Microbiol. Biotechnol. 66:180-186. (Pubitemid 40064312)
22. Sambrook, J. and R. W. Russell (2001) Molecular cloning: A laboratory manual. 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring, NY.
23. Neidhardt, F. C. (1996) Escherichia coli and Salmonella. ASM Press, Washington D. C.
24. Vaara, M. and M. Nurminen (1999) Outer membrane permeability barrier in Escherichia coli mutants that are defective in the late acyltransferases of lipid A biosynthesis. Antimicrob. Agents Chemother. 43:1459-1462. (Pubitemid 29259343)
25. Vorachek-Warren, M. K., S. Ramirez, R. J. Cotter, and C. R. H. Raetz (2002) A triple mutant of Escherichia coli lacking secondary acyl chains on lipid A. J. Biol. Chem. 277:14194-14205. (Pubitemid 34968032)