High expression of β-glucosidase in Bifidobacterium bifidum BGN4 and application in conversion of isoflavone glucosides during fermentation of soy milk

Journal of Microbiology and Biotechnology

Volumn 25, Issue 4, 2015, Pages 469-478

  You, Hyun Ju ^a   Ahn, Hyung Jin ^a   Kim, Jin Yong ^a   Wu, Qian Qian ^a   Ji, Geun Eog ^a,b  

^a Seoul National University   (South Korea)

^b Bifido Co   (South Korea)

Author keywords

Biotransformation; Recombinant Bifidobacterium bifidum BGN4; Soy isoflavones; Soy milk fermentation; glucosidase

Indexed keywords

BETA GLUCOSIDASE; DAIDZIN; GENISTIN; GLUCOSIDE; ISOFLAVONE; ISOFLAVONE GLUCOSIDE; UNCLASSIFIED DRUG; ISOFLAVONE DERIVATIVE;

ARTICLE; BACTERIAL COUNT; BACTERIAL STRAIN; BIFIDOBACTERIUM BIFIDUM; BIOTRANSFORMATION; CONTROLLED STUDY; EXPRESSION VECTOR; FERMENTATION; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; SOYBEAN MILK; THERMOSTABILITY; BIFIDOBACTERIUM; ENZYME STABILITY; GENETICS; METABOLISM; MICROBIAL VIABILITY;

BIFIDOBACTERIUM; BIFIDOBACTERIUM BIFIDUM;

BETA-GLUCOSIDASE; BIFIDOBACTERIUM; BIOTRANSFORMATION; ENZYME STABILITY; FERMENTATION; GENE EXPRESSION; GLUCOSIDES; ISOFLAVONES; MICROBIAL VIABILITY; SOY MILK;

EID: 84928102862     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1408.08013     Document Type: Article

References (43)

1. Am aretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A. 2013. Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl. Microbiol. Biotechnol. 97: 809-817.
2. Ch ampagne CP, Tompkins TA, Buckley ND, Green-Johnson JM. 2010. Effect of fermentation by pure and mixed cultures of Streptococcus thermophilus and Lactobacillus helveticus on isoflavone and B-vitamin content of a fermented soy beverage. Food Microbiol. 27: 968-972.
3. Chien HL, Huang HY, Chou CC. 2006. Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. Food Microbiol. 23: 772-778.
4. Dong H, Rowland I, Yaqoob P. 2012. Comparative effects of six probiotic strains on immune function in vitro. Br. J. Nutr. 108: 459-470.
5. Donkor ON, Shah NP. 2008. Production of beta-glucosidase and hydrolysis of isoflavone phytoestrogens by Lactobacillus acidophilus, Bifidobacterium lactis, and Lactobacillus casei in soymilk. J. Food Sci. 73: M15-M20.
6. Gl eason CE, Carlsson CM, Barnet JH, Meade SA, Setchell KD, Atwood CS, et al. 2009. A p reliminary s tudy o f the safety, feasibility and cognitive efficacy of soy isoflavone supplements in older men and women. Age Ageing 38: 86-93.
7. Hon g KS, Kang HW, Im JP, Ji GE, Kim SG, Jung HC, et al. 2009. Effect of probiotics on symptoms in Korean adults with irritable bowel syndrome. Gut Liver 3: 101-107.
8. Iovine B, Iannella ML, Gasparri F, Giannini V, Monfrecola G, B evilacqua MA. 2012. A c omparative analysis o f the photo-protective effects of soy isoflavones in their aglycone and glucoside forms. Int. J. Mol. Sci. 13: 16444-16456.
9. Kim H, Kwack K, K im D Y, J i GE. 2005. Oral probiotic bacterial administration suppressed allergic responses in an ovalbumin-induced allergy mouse model. FEMS Immunol. Med. Microbiol. 45: 259-267.
10. Kim IH, Park MS, Ji GE. 2003. Characterization of adhesion of Bifidobacterium sp. BGN4 to human enterocyte-like Caco-2 cells. J. Microbiol. Biotechnol. 13: 276-281.
11. Kim JF, Jeong H, Yu DS, Choi SH, Hur CG, Park MS, et al. 2009. Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp. lactis AD011. J. Bacteriol. 191: 678-679.
12. Ki m JY, Kwon JH, Ahn SH, Lee SI, Han YS, Choi YO, et al. 2010. Effect of probiotic mix (Bifidobacterium bifidum, Bifidobacterium lactis, Lactobacillus acidophilus) in the primary prevention of eczema: a double-blind, randomized, placebocontrolled trial. Pediatr. Allergy Immunol. 21: e386-e393.
13. Kim JY, Park MS, Ji GE. 2012. Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells. World J. Gastroenterol. 18: 1308-1318.
14. Kim JY, Wang Y, Park MS, Ji GE. 2010. Improvement of transformation efficiency through in vitro methylation and SacII site mutation of plasmid vector in Bifidobacterium longum MG1. J. Microbiol. Biotechnol. 20: 1022-1026.
15. Kim JY, Wang Y, Park SJ, Ji GE, Park MS. 2012. Cloning and expression of beta-glucosidases from Bifidobacterium lactis AD011. Food Sci. Biotechnol. 21: 731-738.
16. Kim N, Kunisawa J, Kweon MN, Ji GE, Kiyono H. 2007. Oral feeding of Bifidobacterium bifidum (BGN4) prevents CD4+ CD45RBhigh T cell-mediated inflammatory bowel disease by inhibition of disordered T cell activation. Clin. Immunol. 123: 30-39.
17. Ki m ST, Kim HB, Lee KH, Choi YR, Kim HJ, Shin IS, et al. 2012. Steam-dried g inseng b erry f ermented with Lactobacillus plantarum controls the increase of blood glucose and body weight in type 2 obese diabetic db/db mice. J. Agric. Food Chem. 60: 5438-5445.
18. Marazza JA, Garro MS, de Giori GS. 2009. Aglycone production by Lactobacillus rhamnosus CRL981 during soymilk fermentation. Food Microbiol. 26: 333-339.
19. Nunoura N, Ohdan K, Tanaka K, Tamaki H, Yano T, Inui M, et al. 1996. Cloning and nucleotide sequence of the beta-D-glucosidase gene from Bifidobacterium breve clb, and expression of beta-D-glucosidase activity in Escherichia coli. Biosci. Biotechnol. Biochem. 60: 2011-2018.
20. Oti eno DO, Ashton JF, Shah NP. 2007. Isoflavone phytoestrogen degradation in fermented soymilk with selected betaglucosidase producing L. acidophilus strains during storage at different temperatures. Int. J. Food Microbiol. 115: 79-88.
21. Otieno DO, Shah NP. 2007. Endogenous beta-glucosidase and beta-galactosidase activities from selected probiotic microorganisms and their role in isoflavone biotransformation in soymilk. J. Appl. Microbiol. 103: 910-917.
22. Park CS, Yoo MH, Noh KH, Oh DK. 2010. Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases. Appl. Microbiol. Biotechnol. 87: 9-19.
23. Park MS, Kwon B, Shim JJ, Huh CS, Ji GE. 2008. Heterologous expression of cholesterol oxidase in Bifidobacterium longum under the control of 16S rRNA gene promoter of bifidobacteria. Biotechnol. Lett. 30: 165-172.
24. Pham TT, Shah NP. 2008. Effects of lactulose supplementation on the growth of bifidobacteria and biotransformation of isoflavone glycosides to isoflavone aglycones in soymilk. J. Agric. Food Chem. 56: 4703-4709.
25. Pham TT, Shah NP. 2008. Skim milk powder supplementation affects lactose utilization, microbial survival and biotransformation of isoflavone glycosides to isoflavone aglycones in soymilk by Lactobacillus. Food Microbiol. 25: 653-661.
26. Rai mondi S, Roncaglia L, De Lucia M, Amaretti A, Leonardi A, Pagnoni UM, Rossi M. 2009. Bioconversion of soy isoflavones daidzin and daidzein by Bifidobacterium strains. Appl. Microbiol. Biotechnol. 81: 943-950.
27. Ritchie ML, Romanuk TN. 2012. A meta-analysis of probiotic efficacy for gastrointestinal diseases. PLoS One 7: e34938.
28. Rufer CE, Bub A, Moseneder J, Winterhalter P, Sturtz M, Kulling SE. 2008. Pharmacokinetics of the soybean isoflavone daidzein in its aglycone and glucoside form: a randomized, double-blind, crossover study. Am. J. Clin. Nutr. 87: 1314-1323.
29. Set chell KD. 2000. Absorption and metabolism of soy isoflavones-from food to dietary supplements and adults to infants. J. Nutr. 130: 654S-655S.
30. Song X, Xue Y, Wang Q, Wu X. 2011. Comparison of three thermostable beta-glucosidases for application in the hydrolysis of soybean isoflavone glycosides. J. Agric. Food Chem. 59: 1954-1961.
31. Tsangalis D, Ashston JF, McGill AEJ, Shah NP. 2002. Enzymic transformation of isoflavone phytoestrogens in soymilk by β-glucosidase producing bifidobacteria. J. Food Sci. 67: 3104-3113.
32. Tsa ngalis D, Ashton JF, McGill AEJ, Shah NP. 2003. Biotransformation of isoflavones by bifidobacteria in fermented soymilk supplemented with D-glucose and L-cysteine. J. Food Sci. 68: 623-631.
33. Tsu chihashi R, Sakamoto S, Kodera M, Nohara T, Kinjo J. 2008. Microbial metabolism of soy isoflavones by human intestinal bacterial strains. J. Nat. Med. 62: 456-460.
34. v an den Broek LA, Hinz SW, Beldman G, Vincken JP, Voragen AG. 2008. Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics. Mol. Nutr. Food Res. 52: 146-163.
35. Wang Y, Kim JY, Park MS, Ji GE. 2012. Novel Bifidobacterium promoters selected through microarray analysis lead to constitutive high-level gene expression. J. Microbiol. 50: 638-643.
36. Wei QK, Chen TR, Chen JT. 2007. Using of Lactobacillus and Bifidobacterium to product the isoflavone aglycones in fermented soymilk. Int. J. Food Microbiol. 117: 120-124.
37. Wu QQ, You HJ, Ahn HJ, Kwon B, Ji GE. 2012. Changes in growth and survival of Bifidobacterium by coculture with Propionibacterium in soy milk, cow's milk, and modified MRS medium. Int. J. Food Microbiol. 157: 65-72.
38. Xue Y, Yu J, Song X. 2009. Hydrolysis of soy isoflavone glycosides by recombinant beta-glucosidase from hyperthermophile Thermotoga maritima. J. Ind. Microbiol. Biotechnol. 36: 1401-1408.
39. You HJ, Ahn HJ, Ji GE. 2010. Transformation of rutin to antiproliferative quercetin-3-glucoside by Aspergillus niger. J. Agric. Food Chem. 58: 10886-10892.
40. You HJ, Oh DK, Ji GE. 2004. Anticancerogenic effect of a novel chiroinositol-containing polysaccharide from Bifidobacterium bifidum BGN4. FEMS Microbiol. Lett. 240: 131-136.
41. You n SY, Park MS, Ji GE. 2012. Identification of the betaglucosidase gene from Bifidobacterium animalis s ubsp. lactis and its expression in B. bifidum BGN4. J. Microbiol. Biotechnol. 22: 1714-1723.
42. Yu DS, Jeong H, Lee DH, Kwon SK, Song JY, Kim BK, et al. 2012. Complete genome sequence of the probiotic bacterium Bifidobacterium bifidum strain BGN4. J. Bacteriol. 194: 4757-4758.
43. Zh ang YB, Chen WH, Guo JJ, Fu ZH, Yi C, Zhang M, Na XL. 2013. Soy isoflavone supplementation could reduce body weight and improve glucose metabolism in non-Asian postmenopausal women-a meta-analysis. Nutrition 29: 8-14.