Heterologous expression of cholesterol oxidase in Bifidobacterium longum under the control of 16S rRNA gene promoter of bifidobacteria

Biotechnology Letters

Volumn 30, Issue 1, 2008, Pages 165-172

  Park, Myeong Soo ^a,b   Kwon, Bin ^a,c   Shim, Jae Jung ^d   Huh, Chul Sung ^d   Ji, Geun Eog ^a,c  

^a Bifido Co   (South Korea)

^b Anyang Science University   (South Korea)

^c Seoul National University   (South Korea)

^d Bioalpha Inc   (South Korea)

Author keywords

16S rRNA; Bifidobacterium; Cholesterol oxidase; Promoter

Indexed keywords

BACTERIA; BINDING SITES; CHOLESTEROL; GENE EXPRESSION; RNA;

BIFIDOBACTERIUM; CHOLESTEROL OXIDASE; RIBOSOME-BINDING SITE (RBS);

ENZYME ACTIVITY;

CHOLESTEROL OXIDASE; RECOMBINANT PROTEIN; RNA 16S;

ARTICLE; BIFIDOBACTERIUM; CHEMISTRY; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROMOTER REGION; PROTEIN ENGINEERING; STREPTOMYCES COELICOLOR;

BIFIDOBACTERIUM; CHOLESTEROL OXIDASE; ENZYME ACTIVATION; ENZYME STABILITY; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA, RIBOSOMAL, 16S; STREPTOMYCES COELICOLOR;

BIFIDOBACTERIUM; BIFIDOBACTERIUM LONGUM; STREPTOMYCES; STREPTOMYCES COELICOLOR;

EID: 36649006189     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9514-3     Document Type: Article

References (33)

1. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20:470-475
2. Aono R, Doukyu N, Kobayashi H, Nakajima H, Horikoshi K (1994) Oxidative bioconversion of cholesterol by Pseudomonas sp. strain ST-200 in a water-organic solvent two-phase system. Appl Environ Microbiol 60:2518-2523
3. Bourget N, Simonet JM, Decaris B (1993) Analysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsed-field gel electrophoresis genome size estimation and rrn loci number. FEMS Microbiol Lett 110:11-20
4. Brigidi P, Bolognati F, Rossi M, Cerre C, Matteuzzi D (1993) Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli. Lett Appl Microbiol 17:61-64
5. Demuyter P, Leblond P, Decaris B, Simonet JM (1988) Characterization of two families of spontaneously amplifiable units of DNA in Streptomyces ambofaciens. J Gen Microbiol 134:2001-2007
6. Fukuyama M, Miyake Y (1979) Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin. J Biochem 85:1183-1193
7. Gausing K (1980) Regulation of ribosome biosynthesis in E. coli. In: Chambliss G, Craven GR, Davis J, Kahan L, Nomura M (eds) Ribosomes: structure, function, and genetics. University Park Press, Baltimore, pp 693-718
8. Horii M, Ishizaki T, Paik SY, Manome T, Murooka Y (1990) An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp. J Bacteriol 172:3644-3653
9. Jay G, Khoury G, Seth AK, Jay E (1981) Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site. Proc Natl Acad Sci U S A 78:5543-5548
10. Joint FAO/WHO Expert Consultation (2001) Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, October 2001
11. Kaunitz H (1978) Cholesterol and repair processes in arteriosclerosis. Lipids 13:373-374
12. Klaenhammer T, Altermann E, Arigoni F, Bolotin A, Breidt F, Broadbent J, Cano R, Chaillou S, Deutscher J, Gasson M, van de Guchte M, Guzzo J, Hartke A, Hawkins T, Hols P, Hutkins R, Kleerebezem M, Kok J, Kuipers O, Lubbers M, Maguin E, McKay L, Mills D, Nauta A, Overbeek R, Pel H, Pridmore D, Saier M, van Sinderen D, Sorokin A, Steele J, O'Sullivan D, de Vos W, Weimer B, Zagorec M, Siezen R (2002) Discovering lactic acid bacteria by genomics. Antonie Van Leeuwenhoek 82:29-58
13. Kreit J, Lefebvre G, Germain P (1994) Membrane-bound cholesterol oxidase from Rhodococcus sp. cells; production and extraction. J Biotechnol 33:271-282
14. Leahy SC, Higgins DG, Fitzgerald GF, van Sinderen D (2005) Getting better with bifidobacteria. J Appl Microbiol 98:1303-1315
15. Li X, Fu GF, Fan YR, Liu WH, Liu XJ, Wang JJ, Xu GX (2003) Bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: selective inhibitor of angiogenesis and hypoxic tumor growth. Cancer Gene Ther 10:105-111
16. Pang H, Winkler HH (1996) Transcriptional analysis of the 16S rRNA gene in Rickettsia prowazekii. J Bacteriol 178:1750-1755
17. Park MS, Shin DW, Lee KH, Ji GE (1999) Sequence analysis of plasmid pKJ50 from Bifidobacterium longum. Microbiology 145:585-592
18. Park MS, Moon HW, Ji GE (2003) Molecular characterization of plasmid from Bifidobacterium longum. J Microbiol Biotechnol 12:457-462
19. Park MS, Seo JM, Kim JY, Ji GE (2005) Heterologous gene expression and secretion in Bifidobacterium longum. Lait 84:1-8
20. Rhim SR, Park MS, Ji GE (2006) Expression and secretion of Bifidobacterium adolescentis amylase by Bifidobacterium longum. Biotechnol Lett 28:163-168
21. Rossi M, Bridigi P, Gonzalez Vara y Rodriguez A, Matteuzzi D (1996) Characterization of the plasmid pMB1 from Bifidobacterium longum and its use for shuttle vector construction. Res Microbiol 147:133-143
22. Rossi M, Brigidi P, Matteuzzi D (1998) Improved cloning vectors for Bifidobacterium spp. Lett Appl Microbiol 26:101-104
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA
24. Schell MA, Karmirantzou M, Snel B, Vilanova D, Berger B, Pessi G, Zwahlen MC, Desiere F, Bork P, Delley M, Pridmore RD, Arigoni F (2002) The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc Natl Acad Sci U S A 99:14422-14427
25. Shirokano Y, Nakamura K, Mizusawa K (1977) Purification and some properties of an extracellular 3β-hydroxy steroid oxidase produced by Corynebacterium cholesterolicum. J Ferm Technol 55:337-346
26. Smith AG, Brooks CJW (1976) Cholesterol oxidase: properties and application. J Steroid Biochem 7:705-713
27. Sojo M, Bru R, Lopez-Molina D, Garcia-Carmona F, Argulles JC (1997) Cell-linked and extracellular cholesterol oxidase activities from Rhodococcus erythropolis. Isolation and physiological characterization. Appl Microbiol Biotechnol 47:583-589
28. Somkuti GA, Daniel KY, Solaiman DK, Steinber DH (1992) Expression of Streptomyces sp. cholesterol oxidase in Lactobacillus casei. Appl Microbiol Biotechnol 37:330-334
29. Somkuti GA, Solaiman DK, Steinberg DH (1995) Native promoter-plasmid vector system for heterologous cholesterol oxidase synthesis in Streptococcus thermophillus. Plasmid 33:7-14
30. Tomioka H, Kagawa M, Nakamura S (1976) Some enzymatic properties of 3β-hydroxy steroid oxidase produced by Streptomyces violascens. J Biochem 79:903-915
31. Uwajima T, Yagi H, Terada O (1974) Properties of crystalline 3β-hydroxy steroid oxidase of Brevibacterium sterolicum. Agri Biol Chem 38:1149-1156
32. Verma A, Kinger AK, Tyagi JS (1994) Functional analysis of transcription of the Mycobacterium tuberculosis 16S rDNA-encoding gene. Gene 148:113-118
33. Watanabe K, Shimizu H, Aihara H, Nakamura R, Suzuki K, Komagata K (1986) Isolation and identification of cholesterol-degrading Rhodococcus strains from food of animal origin and their cholesterol oxidase activities. J Gen Appl Microbiol 32:137-147