Novel Bifidobacterium promoters selected through microarray analysis lead to constitutive high-level gene expression

Journal of Microbiology

Volumn 50, Issue 4, 2012, Pages 638-643

  Wang, Yan ^a   Kim, Jin Yong ^a   Park, Myeong Soo ^b,c   Ji, Geun Eog ^a,c  

^a Seoul National University   (South Korea)

^b Anyang Science University   (South Korea)

^c Bifido Co   (South Korea)

Author keywords

Bifidobacterium; expression; promoter; vector

Indexed keywords

BETA GLUCOSIDASE;

ARTICLE; BIFIDOBACTERIUM; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE FUSION; GENETICS; METABOLIC ENGINEERING; METHODOLOGY; MICROARRAY ANALYSIS; MICROBIAL GENETICS; PROMOTER REGION; REPORTER GENE;

ARTIFICIAL GENE FUSION; BETA-GLUCOSIDASE; BIFIDOBACTERIUM; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENETICS, MICROBIAL; METABOLIC ENGINEERING; MICROARRAY ANALYSIS; PROMOTER REGIONS, GENETIC;

BIFIDOBACTERIUM; BIFIDOBACTERIUM ANIMALIS SUBSP. LACTIS; BIFIDOBACTERIUM BIFIDUM; ESCHERICHIA COLI;

EID: 84865793203     PISSN: 12258873     EISSN: 19763794     Source Type: Journal    
DOI: 10.1007/s12275-012-1591-x     Document Type: Article

References (20)

1. Bezkorovainy, A. and Miller-Catchpole, R. 1989. Biochemistry and physiology of Bifidobacteria. CRC Press. Boca Raton, Florida, USA.
2. Estrem, S. T., Gaal, T., Ross, W., and Gourse, R. L. 1998. Identification of an UP element consensus sequence for bacterial promoters. Proc. Natl. Acad. Sci. USA95, 9761-9766.
3. Gibson, J. L. and Tabita, F. R. 1996. The molecular regulation of the reductive pentose phosphate pathway in Proteobacteria and Cyanobacteria. Arch. Microbiol. 166, 141-150.
4. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
5. Helmann, J. D. 1995. Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res. 23, 2351-2360.
6. Jensen, P. R. and Hammer, K. 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64, 82-87.
7. Jeong, D. W., Choi, Y. C., Lee, J. M., Kim, J. H., Lee, J. H., Kim, K. H., and Lee, H. J. 2006. Isolation and characterization of promoters from Lactococcus lactis ssp. cremoris LM0230. Food Microbiol. 23, 82-89.
8. Kim, E. B., Piao da, C., Son, J. S., and Choi, Y. J. 2009. Cloning and characterization of a novel tuf promoter from Lactococcus lactis subsp. lactis IL1403. Curr. Microbiol. 59, 425-431.
9. Kim, J. Y., Wang, Y., Park, M. S., and Ji, G. E. 2010. Improvement of transformation efficiency through in vitro methylation and SacII site mutation of plasmid vector in Bifidobacterium longum MG1. J. Microbiol. Biotechnol. 20, 1022-1026.
10. Klijn, A., Moine, D., Delley, M., Mercenier, A., Arigoni, F., and Pridmore, R. D. 2006. Construction of a reporter vector for the analysis of Bifidobacterium longum promoters. Appl. Environ. Microbiol. 72, 7401-7405.
11. Kullen, M. J. and Klaenhammer, T. R. 2000. Genetic modification of intestinal lactobacilli and bifidobacteria. Curr. Issues Mol. Biol. 2, 41-50.
12. McCracken, A., Turner, M. S., Giffard, P., Hafner, L. M., and Timms, P. 2000. Analysis of promoter sequences from Lactobacillus and Lactococcus and their activity in several Lactobacillus species. Arch. Microbiol. 173, 383-389.
13. Mitsuoka, T. 1990. Bifidobacteria and their role in human health. Indust. Microbiol. 6, 263-268.
14. 16S rRNA gene promoter of bifidobacteria. Biotechnol. Lett. 30, 165-172.
15. Park, M. S., Moon, H. W., and Ji, G. E. 2003. Molecular characterization of plasmid from Bifidobacterium longum. J. Microbiol. Biotechnol. 12, 457-462.
16. Rist, M. and Kertesz, M. A. 1998. Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other Gram-negative bacteria. FEMS Microbiol. Lett. 169, 179-183.
17. Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y., USA.
18. Sgorbati, B., Scardovi, V., and Leblanc, D. J. 1982. Plasmids in the genus Bifidobacterium. J. Gen. Microbiol. 128, 2121-2131.
19. Sirard, J. C., Mock, M., and Fouet, A. 1995. Molecular tools for the study of transcriptional regulation in Bacillus anthracis. Res. Microbiol. 146, 729-737.
20. Voskuil, M. I. and Chambliss, G. H. 1998. The -16 region of Bacillus subtilis and other Gram-positive bacterial promoters. Nucleic Acids Res. 26, 3584-3590.