Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 139-160

  Dambournet, D ^a   Hong, S H ^a   Grassart, A ^a   Drubin, D G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Donor plasmid; Endogenous; Fluorescence microscopy; Genome editing; GFP; Nuclease

Indexed keywords

CASPASE 9; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; ZINC FINGER NUCLEASE; DNA; ENDONUCLEASE; HYBRID PROTEIN; PHOTOPROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; CANCER CELL LINE; CELL ISOLATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA METHYLATION; DNA REPAIR; DOUBLE STRANDED DNA BREAK; ENDOCYTOSIS; EXCISION REPAIR; FLUORESCENCE IMAGING; GENE FUSION; GENE LOCUS; GENETIC CODE; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; MAMMAL CELL; MOLECULAR CLONING; MOLECULAR DYNAMICS; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; SINGLE NUCLEOTIDE POLYMORPHISM; STOICHIOMETRY; ANIMAL; ELECTROPORATION; FLUORESCENCE MICROSCOPY; GENE TARGETING; GENETIC ENGINEERING; GENETICS; METABOLISM; PLASMID; PROCEDURES;

MAMMALIA;

ANIMALS; DNA; ELECTROPORATION; ENDONUCLEASES; GENE TARGETING; GENETIC ENGINEERING; GENETIC LOCI; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PLASMIDS; RECOMBINANT FUSION PROTEINS;

EID: 84925387525     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00007-6     Document Type: Chapter

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