Cell biology: Counting cytokinesis proteins globally and locally in fission yeast

Science

Volumn 310, Issue 5746, 2005, Pages 310-314

  Wu, Jian Qiu ^a   Pollard, Thomas D ^a,b  

^a YALE UNIVERSITY   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; IMMUNOLOGY; MICROSCOPIC EXAMINATION; PROTEINS; YEAST;

FLUORESCENCE MICROSCOPY; FUSION PROTEINS; SPINDLE POLE BODIES; YELLOW FLUORESCENT PROTEIN (YFP);

CELL CULTURE;

ACTIN; CELL CYCLE PROTEIN; CELL PROTEIN; HYBRID PROTEIN; PROTEIN CDC12P; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN; BACTERIAL PROTEIN; PHOTOPROTEIN; SCHIZOSACCHAROMYCES POMBE PROTEIN; SIGNAL PEPTIDE; YELLOW FLUORESCENT PROTEIN, BACTERIA;

BIOLOGY;

ARTICLE; CONCENTRATION RESPONSE; CYTOKINESIS; CYTOSKELETON; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SCHIZOSACCHAROMYCES POMBE; SIGNAL TRANSDUCTION; SPINDLE CELL; CHEMISTRY; GENETICS; PROMOTER REGION; SCHIZOSACCHAROMYCES;

SCHIZOSACCHAROMYCES POMBE;

BACTERIAL PROTEINS; CELL CYCLE PROTEINS; CYTOKINESIS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

EID: 26844562643     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1113230     Document Type: Article

References (45)

1. D. N. Robinson, G. Cavet, H. M. Warrick, J. A. Spudich, BMC Cell Biol. 3, 4 (2002).
2. H. N. Higgs, T. P. Pollard, J. Cell Biol. 150, 1311 (2000).
3. S. Ghaemmaghami et al., Nature 425, 737 (2003).
4. D. W. Piston, G. H. Patterson, S. M. Knobel, Methods Cell Biol. 58, 31 (1999).
5. K. Hirschberg, R. D. Phair, J. Lippincott-Schwartz, Methods Enzymol. 327, 69 (2000).
6. M. Dundr, J. G. McNally, J. Cohen, T. Misteli, J. Struct. Biol. 140, 92 (2002).
7. J. Bähler et al., Yeast 14, 943 (1998).
8. J.-Q. Wu, J. R. Kuhn, D. R. Kovar, T. D. Pollard, Dev. Cell 5, 723 (2003).
9. Materials and methods are available as supporting material on Science Online.
10. D. R. Kovar, J.-Q. Wu, T. D. Pollard, Mol. Biol. Cell 16, 2313 (2005).
11. P. Nurse, P. Thuriaux, K. Nasmyth, Mol. Gen. Genet. 146, 167 (1976).
12. M. Desautels, J. P. Den Haese, C. M. Slupsky, L. P. McIntosh, S. M. Hemmingsen, J. Biol. Chem. 276, 5932 (2001).
13. V. M. D'Souza, N. I. Naqvi, H. Wang, M. K. Balasubramanian, Cell Struct. Funct. 26, 555 (2001).
14. M. Lord, T. D. Pollard, J. Cell Biol. 167, 315 (2004).
15. See the fission yeast handbook (available at www. sanger.ac.uk/ PostGenomics/S_pombe/docs/nurse_lab_manual_pdf).
16. F. Sherman, Methods Enzymol. 350, 3 (2002).
17. F. Chang, D. Drubin, P. Nurse, J. Cell Biol. 137, 169 (1997).
18. M. Bezanilla, T. D. Pollard, Mol. Biol. Cell 11, 79 (2000).
19. M. Anderson et al., EMBO J. 21, 5745 (2002).
20. J. J. Tasto, J. L. Morrell, K. L. Gould, J. Cell Biol. 160, 1093 (2003).
21. C. Fankhauser et al., Cell 82, 435 (1995).
22. X. Peng et al., Mol. Biol. Cell 16, 1026 (2005).
23. G. Rustici et al., Nat. Genet. 36, 809 (2004).
24. V. Sirotkin, C. C. Seltener, J.-B. Marchand, T. D. Pollard, J. Cell Biol. 170, 637 (2005).
25. M. Kaksonen, Y. Sun, D. G. Drubin, Cell 115, 475 (2003).
26. T. Takagi, S. A. Ishijima, H. Ochi, M. Osumi, J. Electron Microsc. 52, 161 (2003).
27. M. E. Young, J. A. Cooper, P. C. Bridgman, J. Cell Biol. 166, 629 (2004).
28. A. A. Rodal, L. Kozubowski, B. L. Goode, D. G. Drubin, J. H. Hartwig, Mol. Biol. Cell 16, 372 (2005).
29. M. K. Balasubramanian, E. Bi, M. Glotzer, Curr. Biol. 14, R806 (2004).
30. B. A. Wolfe, K. L. Gould, Trends Cell Biol. 15, 10 (2005).
31. R. Ding, R. R. West, D. M. Morphew, B. R. Oakley, J. R. McIntosh, Mol. Biol. Cell 8, 1461 (1997).
32. A. Grallert, A. Krapp, S. Bagley, V. Simanis, I. M. Hagan, Genes Dev. 18, 1007 (2004).
33. J. L. Morrell et al., Curr. Biol. 14, 579 (2004).
34. J.-Q. Wu, T. D. Pollard, data not shown.
35. D. R. Kovar, T. D. Pollard, Proc. Natl. Acad. Sci. U.S.A. 101, 14725 (2004).
36. R. H. Camahan, K. L. Gould, J. Cell Biol. 162, 851 (2003).
37. T. Kanbe, I. Kobayashi, K. Tanaka, J. Cell Sci. 94, 647 (1989).
38. R. J. Pelham, F. Chang, Nature 419, 82 (2002).
39. K. C. Y. Wong et al., Curr. Biol. 12, 724 (2002).
40. K. Nabeshima et al., Mol. Biol. Cell 9, 3211 (1998).
41. J. Bâhler et al., J. Cell Biol. 143, 1603 (1998).
42. A. Paoletti, F. Chang, Mol. Biol. Cell 11, 2757 (2000).
43. M. Sohrmann, C. Fankhauser, C. Brodbeck, V. Simanis, Genes Dev. 10, 2707 (1996).
44. C. A. Blessing, G. T. Ugrinova, H. V. Goodson, Trends Cell Biol. 14, 435 (2004).
45. We are grateful to M. Morphew and J. R. McIntosh for the generous gift of electron micrographs; K. L. Gould for Arp3p antibodies; J. Lessard for actin antibody; G. Law for help with the spinning disk confocal microscope; and M. Li, X.-Y. Wang, and T. Taylor for help with flow cytometry. We thank M. Manix and L. Zhao for some data analysis; C. Beltzner, D. Kovar, and M. Lord for yeast strains; D. Kovar and V. Sirotkin for actin plasmids; A. Downs for purified S. pombe actin; J. Kuhn, R. Mahaffy, and R. de Rooij for suggestions on microscopy and data analysis; and P. Forscher, P. Dalhaimer, D. Kovar, M. Lord, R. Mahaffy, and V. Sirotkin for comments on the manuscript We are also grateful to other members of the Pollard laboratory for helpful suggestions. This work was supported by NIH Grant GM-26231 to T.D.P. and an Anna Fuller Fund Fellowship to J.-Q.W.