Efficient delivery and functional expression of transfected modified mRNA in human embryonic stem cell-derived retinal pigmented epithelial cells

Journal of Biological Chemistry

Volumn 290, Issue 9, 2015, Pages 5661-5672

  Hansson, Magnus L ^a,d   Albert, Silvia ^a   Somermeyer, Louisa González ^a,b   Peco, Rubén ^a   Mejía Ramírez, Eva ^a   Montserrat, Núria ^a,e   Belmonte, Juan Carlos Izpisua ^c  

^a Center for Regenerative Medicine   (Spain)

^b UNIVERSITY OF BARCELONA   (Spain)

^c SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^d Dept of Cell and Molecular Biology ^*  (Sweden)

^e INSTITUTE FOR BIOENGINEERING OF CATALONIA IBEC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; DISEASE CONTROL; GENE ENCODING; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE THERAPY; GENES; MOBILE SECURITY; OPHTHALMOLOGY; RNA; STEM CELLS; TRANSCRIPTION;

AGE-RELATED MACULAR DEGENERATION; CELL BASED THERAPIES; CELLULAR ENGINEERING; FUNCTIONAL EXPRESSION; FUNCTIONAL IMPROVEMENTS; HUMAN EMBRYONIC STEM CELLS; INNATE IMMUNE RESPONSE; RETINITIS PIGMENTOSA;

CELL ENGINEERING;

MESSENGER RNA; MICROPHTHALMIA ASSOCIATED TRANSCRIPTION FACTOR; CIS TRANS ISOMERASE; INTERFERON REGULATORY FACTOR 3; IRF3 PROTEIN, HUMAN; MITF PROTEIN, HUMAN; OTX2 PROTEIN, HUMAN; RELA PROTEIN, HUMAN; RETINOID ISOMEROHYDROLASE; TRANSCRIPTION FACTOR OTX; TRANSCRIPTION FACTOR RELA;

AGE RELATED MACULAR DEGENERATION; ARTICLE; CELL ENGINEERING; CELL THERAPY; CONTROLLED STUDY; EMBRYONIC STEM CELL; ENGRAFTMENT; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INNATE IMMUNITY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RETINITIS PIGMENTOSA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STARGARDT DISEASE; ACTIVE TRANSPORT; CELL DIFFERENTIATION; CELL LINE; CELL NUCLEUS; CONFOCAL MICROSCOPY; CYTOLOGY; EPITHELIUM CELL; GENETICS; HEK293 CELL LINE; METABOLISM; PROCEDURES; RETINAL PIGMENT EPITHELIUM; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; WESTERN BLOTTING;

ACTIVE TRANSPORT, CELL NUCLEUS; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE; CELL NUCLEUS; CIS-TRANS-ISOMERASES; EMBRYONIC STEM CELLS; EPITHELIAL CELLS; GENE EXPRESSION; HEK293 CELLS; HUMANS; INTERFERON REGULATORY FACTOR-3; MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; OTX TRANSCRIPTION FACTORS; RETINAL PIGMENT EPITHELIUM; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION FACTOR RELA; TRANSFECTION;

EID: 84923775885     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.618835     Document Type: Article

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